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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3,042 results for: GRI;

Showing: 2641 - 2660 of 3042 results

  1. LMP AMPK alpha1 hp1

    Plasmid
    #89489
    Purpose
    shRNA against AMPK alpha1 in mouse/human to knock down protein expression
    Depositor
    Russell Jones
    Article
    Faubert et al Cell Metab. 2013 Jan 8;17(1):113-24. doi
    Insert
    hairpin against mouse/human AMPKalpha1 (PRKAA1, Prkaa1 Human, Mouse)
    Use
    Mouse Targeting and Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 (The GFP-Puro is from a PGK promoter; the shRN…
    Available since
    May 19, 2017
    Availability
    Academic Institutions and Nonprofits only

  6. hEF1α-H2B-mVenus-IRES-mCherry PGK-Puromycin

    Plasmid
    #99278
    Purpose
    Dual fluorescent reporter - constitutive expression of mVenus (515/528) localized in the nucleus, mCherry (587/610) untargeted and visualized in the cytoplasm, and Puromycin resistance
    Depositor
    Jordan Miller
    Article
    Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/
    Inserts
    H2B-mVenus-IRES-mCherry
    Puro
    Use
    Lentiviral
    Tags
    H2B, IRES, PGK, mCherry, and mVenus
    Expression
    Mutation
    Promoter
    EF1 alpha-human and PGK-mouse
    Available since
    Nov. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99278-plasmid-map-sequence-id-262659

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  12. t1-405

    Plasmid
    #166127
    Purpose
    For expression of human talin-1 head (residues 1-405) in E. coli. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.
    Depositor
    Vesa Hytönen
    Article
    Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13
    Insert
    Tln1Head (1-405) (TLN1 Human)
    Use
    Tags
    His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…
    Expression
    Bacterial
    Mutation
    residues 1-405 only
    Promoter
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166127-plasmid-map-sequence-id-330252

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  15. pAAV-CMV-CASANOVA

    Plasmid
    #113036
    Purpose
    AAV vector for CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9
    Depositor
    Dominik Niopek
    Article
    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s
    Insert
    CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
    Use
    AAV, CRISPR, and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    113036-plasmid-map-sequence-id-226953

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  17. AAV2_hSyn_Phobos_CA_Citrine

    Plasmid
    #98218
    Purpose
    Slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue light, inactivation max with 580 nm (accelarates closure to ms). Codon optimized for mammalian expression.
    Depositor
    Simon Wiegert
    Article
    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.
    Insert
    Synthetic construct Phobos_C128A gene
    Use
    AAV
    Tags
    Citrine
    Expression
    Mammalian
    Mutation
    T59S, E83N, E90Q, E101S, V117R, E123S, C128A, T15…
    Promoter
    human synapsin
    Available since
    Aug. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    98218-plasmid-map-sequence-id-256051

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  19. mVenus-proα2(I)-G610C

    Plasmid
    #119839
    Purpose
    Expresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and mVenus between signal sequence and exon 6
    Depositor
    Sergey Leikin
    Article
    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11
    Insert
    Type I procollagen α2 chain (Col1a2 Mouse)
    Use
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    Col1a2 exons 2-5 replaced by fluorescent tag, Gly…
    Promoter
    CMV
    Available since
    Jan. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119839-plasmid-map-sequence-id-233845

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Showing: 2641 - 2660 of 3042 results