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p2xFlag CMV2-YAP2-2nd WW mutant

Plasmid

#19047

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

2xFlag

Expression

Mammalian

Mutation

Tryptophan 258 changed to Alanine, and Proline 2…

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pFRT_TO_FlagHA_USP10

Plasmid

#127102

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

USP10 (USP10 Human)

Expression

Mammalian

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2 GSK3-GFP

Plasmid

#29680

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

glycogen synthase kinase 3

Tags

GFP

Expression

Mammalian

Available Since

July 7, 2011

Availability

Academic Institutions and Nonprofits only
HCMV UL97

Plasmid

#26687

Depositor

Robert Kalejta

Article

Kuny et al PLoS Pathog. 2010 . 6(9):.

Insert

UL97 (UL97 Human Cytomegalovirus (HHV-5))

Tags

HA

Expression

Mammalian

Available Since

Oct. 28, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-SPHK2

Plasmid

#23714

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

SPHK2 (SPHK2 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pFA6a-6xGLY-Strep-tagII-kanMX6

Plasmid

#20757

Depositor

Mark Hochstrasser

Article

Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

Type

Empty backbone

Use

Pcr-based c-terminal tagging

Expression

Yeast

Available Since

March 23, 2009

Availability

Academic Institutions and Nonprofits only

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p2xFLAGhYAP1-WW mutant

Plasmid

#17792

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

2xFlag

Expression

Mammalian

Mutation

Tryptophan 199 to Alanine; Proline 202 to Alanine

Available Since

May 9, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Frb-EGFP-HOTag6

Plasmid

#106919

Purpose

Express Frb-EGFP-HOTag6 fusion in mammalian cells.

Depositor

Xiaokun Shu

Article

Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

Insert

Frb-EGFP-HOTag6

Expression

Mammalian

Available Since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP C3-Lats1-PY1+2 mutant

Plasmid

#19054

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

Insert

Large tumor suppressor kinase 1, N mutant (LATS1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

PY1 mutant, (Tyrosine 376 changed to Alanine) and…

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pHAGE2 mCherry-Atl3

Plasmid

#86680

Purpose

Lentivirus to stably express fluorescent protein in mammalian cells

Depositor

Tom Rapoport

Article

Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

Insert

ATL3 (ATL3 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2017

Availability

Academic Institutions and Nonprofits only

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IRES-GFP-AXL-KD

Plasmid

#65498

Purpose

Expresses kinase dead AXL in mammalian cells with cistronic EGFP

Depositor

Aaron Meyer

Article

Meyer et al Cell Syst. 2015 Jul 29;1(1):25-36.

Insert

Tyrosine-protein kinase receptor UFO (AXL Human)

Tags

FLAG

Expression

Mammalian

Mutation

K567R

Promoter

CMV

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pAC91-pmax-dCas9VP64

Plasmid

#48223

Purpose

dCas9VP64 on pmax expression vector

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9(D10A;H840A) fusion with VP64 activation domain

Use

CRISPR

Tags

HA Tag and VP64

Expression

Mammalian

Mutation

D10A;H840A (catalytically inactive)

Promoter

CAGGS

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pBJ5 BRG1 DN

Plasmid

#17874

Depositor

Jerry Crabtree

Article

Khavari et al Nature. 1993 Nov 11. 366(6451):170-4.

Insert

Brg DN (SMARCA4 Human)

Expression

Mammalian

Mutation

dominant negative

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only
pDONR223-DAPK1

Plasmid

#23480

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

DAPK1 (DAPK1 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pGEX4T-hLIG3

Plasmid

#81055

Purpose

bacterial expression of human LIG3

Depositor

Primo Schaer

Article

Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

Insert

DNA ligase 3 (LIG3 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pJFRC172-10XUAS-loxP>-dSTOP-loxP>-myr::GFP

Plasmid

#32145

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

loxP> STOP STOP loxP>

Expression

Insect

Available Since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only

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pDONR223-YES1

Plasmid

#23938

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

YES1 (YES1 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA4-Beclin1 1-150

Plasmid

#24389

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

Beclin1 (BECN1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains amino acids 1-150.

Available Since

April 1, 2010

Availability

Academic Institutions and Nonprofits only
pLysscys255

Plasmid

#36429

Depositor

Emine Gunhan, Pete Lollar

Article

Gunhan et al Protein Expr Purif. 2008 Apr;58(2):203-9

Insert

Saporin-3/cys255

Expression

Bacterial

Mutation

cys added at position 255

Promoter

T7

Available Since

May 10, 2012

Availability

Academic Institutions and Nonprofits only

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2HA-pDEST-C

Plasmid

#118374

Purpose

Gateway compatible mammalian expression vector with C-terminal 2HA to tag proteins of interest.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

2HA

Expression

Mammalian

Available Since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

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