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pDONR223_BIRC6

Plasmid

#197974

Purpose

Entry clone for wt BIRC6

Depositor

Eric Fischer

Article

Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

Insert

BIRC6 (BIRC6 Human)

Use

Other

Mutation

L1332V

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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CMV-Cx35-Halo-C-end

Plasmid

#71512

Purpose

Mammalian expression plasmid encoding perch Cx35 with a C-terminal HaloTag.

Depositor

John O'Brien

Article

Wang et al J Cell Sci. 2015 Nov 1;128(21):3888-97.

Insert

Connexin 35

Tags

HaloTag

Expression

Mammalian

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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6xHis(TEV)-Drp1(K38A)-StrepII

Plasmid

#174423

Purpose

Expresses human Drp1 Isoform 3 with K38A mutation in bacteria

Depositor

Thomas Pucadyil

Article

Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

Insert

Drp1

Tags

6xHis(TEV) and StrepII

Expression

Bacterial

Mutation

K38A

Promoter

T7

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_P0526-sgRNAmreB

Plasmid

#149654

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi mreB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAmreB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, P0526

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-2T-RNAPII-CTD wt #14-HA

Plasmid

#160688

Purpose

Expression of GST-RNA Polymerase II C-terminal domain fusion protein. Contains 14 of the consensus heptapeptide repeats shown to be phosphorylated by Cdc2 kinase.

Depositor

Jeffry Corden

Article

Patturajan et al J Biol Chem. 1998 Feb 20;273(8):4689-94.

Insert

RNA Polymerase II CTD

Expression

Bacterial

Mutation

C terminal domain only

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry-miniSOG-N1

Plasmid

#54803

Purpose

Localization: N1 Cloning Vector, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Type

Empty backbone

Tags

mCherry-miniSOG

Expression

Mammalian

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only
CROP_C9_Puro_DNMT3B

Plasmid

#183284

Purpose

All-in-One CRISPRko system with a guide RNA that targets DNMT3B gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

DNMT3B

Use

Lentiviral

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLIPE-1m

Plasmid

#13544

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E A207W

Tags

ECFP and Venus

Expression

Mammalian

Mutation

A207W in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pSUPER.ATRi

Plasmid

#14582

Depositor

Didier Trono

Article

Ariumi et al J Virol. 2005 Mar . 79(5):2973-8.

Insert

ATR shRNA (ATR Human)

Use

RNAi

Expression

Mammalian

Available Since

March 27, 2007

Availability

Academic Institutions and Nonprofits only
pENTR-PpU6P-sgRNA-L1L2

Plasmid

#113735

Purpose

Gateway entry vector containing attL1 and attL2 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

PpU6P::sgRNA

Use

CRISPR; Gateway entry vector

Promoter

P. patens U6 promoter

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_PARP1

Plasmid

#183312

Purpose

All-in-One CRISPRko system with a guide RNA that targets PARP1 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

PARP1

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3 T7 Bmf

Plasmid

#24257

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bmf (BMF Human)

Tags

T7

Expression

Mammalian

Available Since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
T7RNAP Cyslight V174C

Plasmid

#205078

Purpose

T7RNAP protein expression

Depositor

Smita Patel

Article

Koh et al Mol Cell. 2018 May 17;70(4):695-706.e5.

Insert

T7RNAP Cyslight V174C (T7p07 Bacteriophage T7)

Expression

Bacterial

Mutation

C125S, C347S, C510S, C515S, C530S, C723S V174C

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_DNMT3A

Plasmid

#183283

Purpose

All-in-One CRISPRko system with a guide RNA that targets DNMT3A gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

DNMT3A

Use

Lentiviral

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pET22b-GFP-CPDSalI

Plasmid

#38257

Depositor

Matthew Bogyo, Aimee Shen

Article

Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

Insert

eGFP

Tags

6xHis and Vibrio cholerae MARTX toxin cysteine pr…

Expression

Bacterial

Mutation

cloned from pEGFPN3 (Clonetech)

Promoter

T7

Available Since

July 20, 2012

Availability

Academic Institutions and Nonprofits only
pGSTag Bmf

Plasmid

#24264

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bmf (BMF Human)

Tags

Gst

Expression

Bacterial

Available Since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
pENTR-PpU6P-sgRNA-L5L2

Plasmid

#113738

Purpose

Gateway entry vector containing attL5 and attL2 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

PpU6P::sgRNA

Use

CRISPR; Gateway entry vector

Promoter

P. patens U6 promoter

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pTYF-sGFAP-IRES-tdTomato

Plasmid

#166920

Purpose

Empty plasmid backbone; includes IRES-tdTomato and sGFAP promoter

Depositor

Sergey Kasparov

Article

Vaccari Cardoso et al Glia. 2021 Jan 5. doi: 10.1002/glia.2396

Type

Empty backbone

Use

Lentiviral

Tags

tdTomato

Expression

Mammalian

Promoter

sGFAP

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2-MT-mitfa

Plasmid

#81231

Purpose

expression of zebrafish mitfa

Depositor

James Lister, David Raible

Article

Lister et al Development. 1999 Sep;126(17):3757-67.

Insert

mitfa (mitfa Zebrafish)

Use

Zebrafish expression

Tags

6 myc tags

Expression

Mammalian

Mutation

contains amino acids 5-412

Promoter

sCMV IE94 promoter

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pBS Wnt-3aHA

Plasmid

#17374

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-3aHA (Wnt3a Mouse)

Tags

HA

Expression

Bacterial

Available Since

July 7, 2008

Availability

Academic Institutions and Nonprofits only