We narrowed to 7,024 results for: tet

1. pTRE/SP M87 S245X

   Plasmid

   #92374

   Purpose

   Inducible expression of M87 S245X spastin in mammalian cells

   Depositor

   Peter Baas

   Article

   Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

   Insert

   spastin (SPAST Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   1-460 bp deleted, S245X

   Promoter

   Tet-responsive P tight

   Available since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTRE/SP M87C448Y

   Plasmid

   #89329

   Purpose

   Inducible expression of M87 C448Y spastin in mammalian cells

   Depositor

   Peter Baas

   Article

   Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

   Insert

   spastin (SPAST Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   1-460 bp deleted, C448Y

   Promoter

   Tet-responsive P tight

   Available since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTRE/SP C448Y with 5'UTR

   Plasmid

   #89325

   Purpose

   Inducible expression of C448Y spastin M1 and M87 in mammalian cells

   Depositor

   Peter Baas

   Article

   Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

   Insert

   spastin (SPAST Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   C448Y

   Promoter

   Tet-responsive P tight

   Available since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTRE/SP M1C448Y

   Plasmid

   #89326

   Purpose

   Inducible expression of M1C448Y spastin in mammalian cells

   Depositor

   Peter Baas

   Article

   Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

   Insert

   spastin (SPAST Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   1-194 bp of 5'UTR deleted, C448Y

   Promoter

   Tet-responsive P tight

   Available since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFRT-TODestFLAGHA_RBPMS_R38E

   Plasmid

   #65665

   Purpose

   FLAG-HA expression vector with RBPMS carrying mutation R38E

   Depositor

   Thomas Tuschl

   Article

   Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

   Insert

   RBPMS (RBPMS Human)
   

   Use

   Tags

   FLAG HA

   Expression

   Mammalian

   Mutation

   changed arginine 38 to glutamic acid

   Promoter

   CMV, 2x Tet

   Available since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFRT-TODestFLAGHA_RBPMS_R38Q

   Plasmid

   #65094

   Purpose

   Destination vector with RBPMS with R38Q mutation for stable cell line generation

   Depositor

   Thomas Tuschl

   Article

   Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

   Insert

   RBPMS (RBPMS Human)
   

   Use

   Tags

   FLAG HA

   Expression

   Mammalian

   Mutation

   changed arginine 38 to glutamine

   Promoter

   CMV, 2x Tet

   Available since

   June 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFRT-TODestFLAGHA_RBPMS_R38A_E39A

   Plasmid

   #65664

   Purpose

   FLAG-HA expression vector with RBPMS carrying mutation R38A, E39A

   Depositor

   Thomas Tuschl

   Article

   Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

   Insert

   RBPMS (RBPMS Human)
   

   Use

   Tags

   FLAG HA

   Expression

   Mammalian

   Mutation

   changed arginine 38 to alanine, and glutamic acid…

   Promoter

   CMV, 2x Tet

   Available since

   June 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. MC177VSG221RNAi

   Plasmid

   #59730

   Purpose

   For use in trypanosome cell lines already expressing T7 polymerase and Tet-repressor. Tetracycline induction results in the production of dsRNA targeting VSG221.

   Depositor

   Gloria Rudenko

   Article

   Sheader et al Proc Natl Acad Sci U S A. 2005 Jun 14;10

   Insert

   VSG221 RNAi fragment
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   Promoter

   opposing T7 promoters

   Available since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB-TO-ASCL1-DLX2

   Plasmid

   #182307

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into GABAergic neuron via ASCL1 and DLX2 expression

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Inserts

   Ascl1 (Ascl1 Mouse)
   Dlx2 (Dlx2 Mouse)
   puroR-T2A-mycNLS-mTagBFP2
   

   Use

   Tags

   None and T2A-mycNLS-mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a and pTRE3G-bidirectional

   Available since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTRE-Tight-MitoTimer

    Plasmid

    #50547

    Purpose

    Expresses the Timer protein targeted to the mitochondria under a Tet-inducible promoter

    Depositor

    Roberta Gottlieb

    Article

    Hernandez et al Autophagy. 2013 Nov 1;9(11):1852-61. doi

    Insert

    MitoTimer
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    F12S mutation in Timer

    Promoter

    Available since

    March 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE TRE dCas9-VP64

    Plasmid

    #50916

    Purpose

    Tet-regulatable dCas9-VP64 2nd generation lentiviral expression vector

    Depositor

    Scot Wolfe, Rene Maehr

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and VP64

    Expression

    Mammalian

    Mutation

    D10A, H840A nuclease-deficient

    Promoter

    TRE

    Available since

    March 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE TRE dCas9-KRAB

    Plasmid

    #50917

    Purpose

    Tet-regulatable dCas9-KRAB 2nd generation lentiviral expression vector

    Depositor

    Scot Wolfe, Rene Maehr

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and KRAB

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive)

    Promoter

    TRE

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-TRE-mNeonGreen

    Plasmid

    #99131

    Purpose

    An AAV genome with tet-inducible expression of the fluorescent protein mNeonGreen

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    mNeonGreen
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pICE-HA-MRE11-WT

    Plasmid

    #82033

    Purpose

    Plasmid for constitutive or doxycycline-inducible expression of wild-type human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Patrick Calsou, Sébastien Britton

    Article

    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

    Insert

    MRE11 (MRE11 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-tet

    Available since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCW57.1-DUX4-CA

    Plasmid

    #99281

    Purpose

    All-in-one lentivector with tet-inducible DUX4 (codon sequences altered)

    Depositor

    Stephen Tapscott

    Article

    Jagannathan et al Hum Mol Genet. 2016 Oct 15;25(20):4419-4

    Insert

    DUX4 (codon altered) (DUX4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    codon altered

    Promoter

    TRE

    Available since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-TRE-eYFP

    Plasmid

    #104056

    Purpose

    An AAV genome with tet-inducible expression of the fluorescent protein eYFP

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Insert

    EYFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    TRE

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. T7 promoter + terminators reporter plasmid

    Plasmid

    #156456

    Purpose

    A mutational reporter plasmid that can gain Kanamycin and/or Tetracycline resistance upon mutation. Contains T7 promoter and a terminator array.

    Depositor

    Matthew D. Shoulders

    Article

    Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

    Inserts

    NeoR/KanR
    TcR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    ATG start codon mutated to ACG

    Promoter

    Em7 and tet

    Available since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCW57.1-Luciferase

    Plasmid

    #99283

    Purpose

    All-in-one lentivector with tet-inducible luciferase

    Depositor

    Stephen Tapscott

    Article

    Shadle et al PLoS Genet. 2017 Mar 8;13(3):e1006658. d

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNTI821

    Plasmid

    #216630

    Purpose

    Tet-inducible Cal-ID (V5) for Sleeping Beauty transposon system

    Depositor

    Nicholas Ingolia

    Article

    Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

    Insert

    Cal-ID
    

    Use

    Sleeping beauty transposon system

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSLIK-GFP

    Plasmid

    #66844

    Purpose

    Tet-inducible expression of GFP in mammalian cells

    Depositor

    Christian Metallo

    Article

    Lewis et al Mol Cell. 2014 Jul 17;55(2):253-63. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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