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pX459_gRNA-AAVS1_hspCas9

Plasmid

#193309

Purpose

Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)

Depositor

Simon Elsaesser

Article

Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

Insert

Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)

Use

CRISPR

Expression

Mammalian

Mutation

not applicable

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5 alpha6

Plasmid

#16036

Depositor

Filippo Giancotti

Article

Dans et al J Biol Chem. 2001 Jan 12. 276(2):1494-50

Insert

alpha6 (ITGA6 Human)

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Nuc-ExRai-AMPKAR

Plasmid

#192450

Purpose

ExRai-AMPKAR targeted to the nucleus.

Depositor

Jin Zhang

Article

Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

Insert

ExRai-AMPKAR-NLS

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K63

Plasmid

#17606

Purpose

Mammalian expression of HA tagged ubiquitin with K63 only, other lysines mutated to arginines

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K63 only, other lysines mutated to arginines. Enh…

Available Since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-Tp-gfpmut3

Plasmid

#65033

Purpose

TpR mini-Tn7 vector, mobilizable, for GFP tagging bacteria

Depositor

Herbert Schweizer

Article

Choi et al Nat Protoc. 2006;1(1):153-61.

Insert

gfpmut3

Expression

Bacterial

Available Since

July 15, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-PE7-SB2

Plasmid

#228868

Purpose

PE7 connected to MLH1-SB and MLH1-SB-NLS via 2A sequence

Depositor

Sangsu Bae

Article

Park et al Cell. 2025 Aug 1:S0092-8674(25)00799-8.

Insert

PE7-2A-MLH1-SB-NLS-2A-MLH1-SB

Use

CRISPR

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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EF1a_mCherry_P2A_Hygro

Plasmid

#135003

Purpose

Lentiviral vector to express mCherry or to clone and express gene of interest, under control of EF1a promoter. Barcodes compatible with detection in single cell RNA-seq may be inserted at HpaI site.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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TFORF2069

Plasmid

#141902

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

MYC (MYC Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pNP2916 [Cloning backbone, bRNA Landing Pad]

Plasmid

#247254

Purpose

Cloning backbone for cloning bridge RNA

Depositor

Patrick Hsu

Article

Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pNP3613 [Cloning backbone, recombinase]

Plasmid

#247264

Purpose

Cloning backbone for cloning bridge recombinase

Depositor

Patrick Hsu

Article

Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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