We narrowed to 11,959 results for: pcDNA

1. pcDNA3-sACE2v2.4-sfGFP

   Plasmid

   #149666

   Purpose

   Mammalian expression plasmid for sACE2-sfGFP high affinity variant 2.4

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Use

   Tags

   Linker GSGGSGSGG and superfolder GFP

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease domain (a.a. M1-D…

   Promoter

   CMV

   Available since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Carl-ReNL-KDEL_pcDNA3

   Plasmid

   #89535

   Purpose

   Red color variant of bright luminescent protein, targeted to the endoplasmic reticulum

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   ReNL
   

   Use

   Tags

   Calreticulin and KDEL

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-LifeAct-Venus

   Plasmid

   #87613

   Purpose

   Actin-targeted Venus (YFP).

   Depositor

   Jin Zhang

   Article

   Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

   Insert

   LifeAct-Venus
   

   Use

   Tags

   17-aa sequence from Abp140 (aka "Lifeact&quo…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5-GFP-PKHD1

   Plasmid

   #108408

   Purpose

   Can be used to generate stable Flp-In cells with tetracycline-inducible human polyduction/fibrocystin expression.

   Depositor

   Gregory Germino

   Article

   Kaimori et al Hum Mol Genet. 2007 Apr 15. 16(8):942-56

   Insert

   PKHD1 (PKHD1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pY128 (pcDNA3.1-U6-huMb2Cpf1)

   Plasmid

   #92304

   Purpose

   Expression of humanized Mb2Cpf1 and crRNA

   Depositor

   Feng Zhang

   Insert

   huMb2Cpf1
   

   Use

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1 Flag-Eya2

   Plasmid

   #49264

   Purpose

   expresses Flag tagged Eya2

   Depositor

   Heide Ford

   Insert

   Eya2 (EYA2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA5-FRT-TO-Nsp12

   Plasmid

   #157709

   Purpose

   mammalian expression of untagged SARS-CoV-2 Nsp12 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp12 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   Available since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pcDNA3.1-Firefly-ccdB

   Plasmid

   #87069

   Purpose

   Mammalian Gateway-compatible expression vector backbone with an N-terminal Firefly luciferase

   Depositor

   Mikko Taipale

   Type

   Empty backbone

   Use

   Tags

   Firefly luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3 IL-2 signal mFc

   Plasmid

   #128042

   Purpose

   Mammalian expression vector with C terminal IL-2 signal peptide and mFc

   Depositor

   Oskar Laur

   Type

   Empty backbone

   Use

   Tags

   IL-2 signal peptide and mFc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-H2B-Venus

    Plasmid

    #87614

    Purpose

    Venus (YFP) fused to histone H2B.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    H2B-Venus (H2BC8 Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA-FRT-FAK10

    Plasmid

    #59119

    Purpose

    FAK fret sensor with 10 nm helix

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

    Insert

    Focal Adhesion Kinase
    

    Use

    Tags

    FLAG, mCerulean, and mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. alpha-0 CFP in pcDNA3.1

    Plasmid

    #36396

    Depositor

    Narasimhan Gautam

    Article

    Azpiazu et al J Biol Chem. 2004 Jun 25;279(26):27709-1

    Insert

    G protein alpha 0 (Gnao1 Rat)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    CFP inserted into the KpnI-SpeI sites engineered …

    Promoter

    cmv

    Available since

    Feb. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pZ143-pcDNA3-BvCas12b

    Plasmid

    #122445

    Purpose

    Expresses BvCas12b in mammalian cells

    Depositor

    Feng Zhang

    Article

    Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

    Insert

    CMV-NLS-BvCas12b-NLS-3xHA
    

    Use

    Tags

    3XHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(+) Drp1 V2 S600A

    Plasmid

    #46344

    Depositor

    David Chan

    Article

    Loson et al Mol Biol Cell. 2013 Jan 2.

    Insert

    Drp1 V2 (Dnm1l Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S600A

    Promoter

    CMV

    Available since

    July 23, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3.1(+) Drp1 V2 S600D

    Plasmid

    #46345

    Depositor

    David Chan

    Article

    Loson et al Mol Biol Cell. 2013 Jan 2.

    Insert

    Drp1 V2 (Dnm1l Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S600D

    Promoter

    CMV

    Available since

    July 23, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.1-MS2-HA-HsSmg7_T

    Plasmid

    #147556

    Purpose

    Mammalian Expression of HsSmg7

    Depositor

    Elisa Izaurralde

    Article

    Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

    Insert

    HsSmg7 (SMG7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    two silent mutation G216A, A1521G and one non sil…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-Lyso-AktAR2

    Plasmid

    #64933

    Purpose

    Lysosome-targeted FRET biosensor for monitoring Akt activity; targeted to cytosolic face of lysosomal membrane.

    Depositor

    Jin Zhang

    Article

    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

    Insert

    LAMP1-AktAR2 (LAMP1 Synthetic, Human, Budding Yeast)
    

    Use

    Tags

    Cerulean3, Full-length LAMP1, and cpVenus[E172]

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5/FRT/TO V5 DNAJB7

    Plasmid

    #19530

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DnaJ (Hsp40) homolog, subfamily B, member 7 (DNAJB7 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA-HSF1Δ∼PRD

    Plasmid

    #71725

    Purpose

    plasmid expressing mic-his tag phosphorylation-deficient hHSF1

    Depositor

    Lea Sistonen

    Article

    Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

    Insert

    phosphorylation-deficient HSF1 (HSF1 Human)
    

    Use

    Tags

    mac 6xHIS

    Expression

    Mammalian

    Mutation

    15 phosphorylation sites in RD mutated to Alanine

    Promoter

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-MS2-HA-HsNot4_AA

    Plasmid

    #148255

    Purpose

    Mammalian Expression of HsNot4

    Depositor

    Elisa Izaurralde

    Article

    Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

    Insert

    HsNot4 (CNOT4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one silent mutation compared to the sequence give…

    Promoter

    Available since

    Nov. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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