We narrowed to 13,694 results for: NTS

1. Sup35 Sc/Ca NM Chimera 4 G/A His7 Cys

   Plasmid

   #15602

   Depositor

   Susan Lindquist

   Article

   Tessier et al Nature. 2007 May 31. 447(7144):556-61.

   Insert

   Sup35 NM (SUP35 Candida albicans, Budding Yeast)
   

   Use

   Tags

   7xHis and Cysteine

   Expression

   Bacterial

   Mutation

   4 G/A. Four glycines mutated to alanines. The pos…

   Promoter

   Available since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMIG small T 88

   Plasmid

   #10691

   Depositor

   Bob Weinberg

   Article

   Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

   Insert

   SV40 small T Antigen 88 (SV40gp7 simian virus)
   

   Use

   Retroviral

   Tags

   IRES GFP

   Expression

   Mammalian

   Mutation

   contains only the N-termial 88 amino acids

   Promoter

   Available since

   Sept. 23, 2005

   Availability

   Academic Institutions and Nonprofits only

3. pRK5_tAKARδ

   Plasmid

   #119916

   Purpose

   Mammalian expression of the tAKARδ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

   Depositor

   Haining Zhong

   Article

   Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

   Insert

   tAKARδ
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRK5_tAKARε

   Plasmid

   #119917

   Purpose

   Mammalian expression of the tAKARε sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

   Depositor

   Haining Zhong

   Article

   Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

   Insert

   tAKARε
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBABE-hygro-mTurquoise2-Nuf2

   Plasmid

   #80762

   Purpose

   Express Nuf2 N-terminally labeled with mTurquoise2

   Depositor

   Daniel Needleman

   Article

   Yoo et al Elife. 2018 Jul 25;7. pii: 36392. doi: 1

   Insert

   Nuf2 (NUF2 Human)
   

   Use

   Retroviral

   Tags

   mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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6. lentiCRISPR-v2-HygR-mCherry

   Plasmid

   #167189

   Purpose

   Lentiviral expression of sgRNA with hygromycin resistance gene and mCherry

   Depositor

   Yin Shen

   Article

   Ren et al Sci Adv. 2021 Sep 17;7(38):eabi4360. doi

   Insert

   Hygromycin B phosphotransferase and mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. M50 Super 8x TOPFlash

   Plasmid

   #12456

   Purpose

   Beta-catenin reporter. TCF/LEF sites upstream of a luciferase reporter.

   Depositor

   Randall Moon

   Article

   Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

   Insert

   TCF/LEF binding sites (Ctnnb1 )
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 13, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. N8his-GFPenhancer-GGGGS4-LaG16

   Plasmid

   #140442

   Purpose

   We designed a flexible linker to connect two types of anti GFP nanobodies and used this fusion nanobody to purify GFP tagged protein.

   Depositor

   Motoyuki Hattori

   Article

   Zhang et al Sci Rep. 2020 Apr 10;10(1):6239. doi: 10

   Insert

   GFPenhancer-GGGGS4-LaG16
   

   Use

   Tags

   8 histidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promotor

   Available since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. Fragment1 with TLS2

   Plasmid

   #196983

   Purpose

   Gateway-compatible gRNA backbone with TLS2 mobility signal. Template for the addition of target sequences to produce 2x graft-mobile gRNA-TLS2.

   Depositor

   Friedrich Kragler

   Article

   Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   Intermediate fragment for assembly of gRNA-TLS2 construct
   

   Use

   Gateway-compatible entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBR BsmAIM Kan + p15A BsaIR Amp

    Plasmid

    #206208

    Purpose

    Contains two plasmids. Constitutive expression of BsmAIM and inducible expression of BsaIR

    Depositor

    Andrew Ellington

    Article

    Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

    Insert

    BsamAIM and BsaIR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGWB441

    Plasmid

    #74827

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with eYFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGWB442

    Plasmid

    #74828

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with eYFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGWB515

    Plasmid

    #74857

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with 3xHA (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJAB988

    Plasmid

    #133920

    Purpose

    miniICE-tet(M)cat(PC194)_PT7-GFP; used to integrate into JAB1000 B. subtilis XPORT strain with IPTG-inducible GFP under T7 promoter

    Depositor

    Christopher Voigt

    Article

    Brophy et al Nat Microbiol. 2018 Sep;3(9):1043-1053.

    Insert

    miniICE-tet(M)cat(PC194)_PT7-GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pmCherry_sgRNA-ver2

    Plasmid

    #126776

    Purpose

    gRNA expression vector with mCherry transfection control, does not contain the direct repeat of the truncated guideRNA

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    sgRNA (for SpCas9)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMK435

    Plasmid

    #236573

    Purpose

    Cloning vector for PaqCI Golden Gate assembly of ISYmu1 gRNAs into TRV2 Architecture_B

    Depositor

    Steven Jacobsen

    Article

    Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-SpRY-ABE8e

    Plasmid

    #185671

    Purpose

    Expresses SpRY-ABE8e in mammalian cells

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    ecTadA(8e)-nSpRY
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    SpRY D10A

    Promoter

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLS-SceI

    Plasmid

    #137725

    Purpose

    Lentivirus reporter assay plasmid that contains a I-SceI site and a EGFP reporter gene.

    Depositor

    Nadav Ahituv

    Article

    Gordon et al Nat Protoc. 2020 Aug;15(8):2387-2412. do

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGWB454

    Plasmid

    #74836

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with mRFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA-3xHA-hTERT

    Plasmid

    #51637

    Purpose

    Mammalian expression of 3xHA tagged human TERT, N-terminal.

    Depositor

    Steven Artandi

    Article

    Venteicher et al Cell. 2008 Mar 21;132(6):945-57. doi: 10

    Insert

    TERT (TERT Human)
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. LV_EF1a_SV40_Large_T_Antigen-P2A-Hygro_Barcode

    Plasmid

    #170255

    Purpose

    Barcoded lentiviral vector to express SV40 Large T Antigen in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    SV40 Large T Antigen
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDECKO_UCA1

    Plasmid

    #72628

    Purpose

    Expresses two gRNAs targeting the UCA1 promoter

    Depositor

    Rory Johnson, Roderic Guigo

    Article

    Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

    Insert

    gRNAs toward UCA1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (gRNA1) and H1 (gRNA2)

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEF-Bos TRIF Flag

    Plasmid

    #41550

    Depositor

    Kate Fitzgerald, Tom Maniatis

    Article

    Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

    Insert

    Trif (TICAM1 Human)
    

    Use

    Tags

    6xHis and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

24. Lenti-EFS-PEmax-P2A-Puro

    Plasmid

    #228890

    Purpose

    Lentiviral delivery and expression of PEmax protein with puromycin selection cassette.

    Depositor

    Francisco J Sánchez-Rivera

    Article

    Gould et al Nat Biotechnol. 2024 Mar 12. doi: 10.103

    Inserts

    PEmax
    Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA5/FRT/TO_H2B-HaloTag10-T2A-Tomm20-HaloTag9

    Plasmid

    #175525

    Purpose

    Co-expression of HaloTag10 (nuclear) and 9 (mitochondrial surface) in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    H2B-HaloTag10 and Tomm20-HaloTag9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag10 = HaloTag7-Q165W and HaloTag9 = HaloTag…

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pENTR-nlsABACUS2-400n

    Plasmid

    #203726

    Purpose

    Gateway entry vector for the the nlsABACUS2-400n biosensor for (+)-Abscisic acid

    Depositor

    Alexander Jones

    Article

    Rowe et al Nat Plants. 2023 Jun 26. doi: 10.1038/s4

    Insert

    nlsABACUS2-400n
    

    Use

    Synthetic Biology

    Tags

    Myc

    Expression

    Mutation

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDGE687

    Plasmid

    #167299

    Purpose

    Vector for plant genome editing containing p35S:GFP-Cas9 expression cassette, nptII selection marker, and SlFAST2 and Ca-Bs3 counterselction markers, but not sgRNAs.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLS-SV40-mP-EGFP

    Plasmid

    #137724

    Purpose

    Lentivirus based EGFP expression vector

    Depositor

    Nadav Ahituv

    Article

    Gordon et al Nat Protoc. 2020 Aug;15(8):2387-2412. do

    Insert

    SV40 enhancer
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGWB455

    Plasmid

    #74837

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with mRFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Pb-TRE3G-SpCas9-IRES-blast

    Plasmid

    #195506

    Purpose

    Inducible Piggybac vector expressing SpCas9 with Blasticidin selection

    Depositor

    Joanna Wysocka

    Article

    Bajpai et al Science. 2023 Aug 11;381(6658):eade6289.

    Insert

    spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBABE-neo largeTcDNA

    Plasmid

    #1780

    Purpose

    Retroviral expression of Large T Antigen. Used to create immortalized cell lines.

    Depositor

    Bob Weinberg

    Article

    Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

    Insert

    large T antigen (SV40gp6 simian virus)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 3, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pGWB433

    Plasmid

    #74824

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with GUS (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. dCas13d-BFP

    Plasmid

    #215720

    Purpose

    Catalytically inactive dCasRx with 2A-BFP

    Depositor

    Chaolin Zhang

    Article

    Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

    Insert

    dCasRx
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pK7WG2-mitoTAL2-orf125

    Plasmid

    #158737

    Purpose

    Plant mitoTALEN vector to cut Brassica CMS gene orf125 (orf138)

    Depositor

    Shin-ichi Arimura

    Article

    Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

    Insert

    mitoTALEN left and right to target orf125 (orf138)
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pTA7002-avrPto

    Plasmid

    #49156

    Purpose

    Plant transformation binary vector for Dexamethasone inducible expression of the type III effector gene avrPto

    Depositor

    Sheng Yang He

    Article

    Hauck et al Proc Natl Acad Sci U S A. 2003 Jul 8;100

    Insert

    avrPto
    

    Use

    Plant transformation binary vector

    Tags

    Expression

    Mutation

    Promoter

    dexamethasone inducible

    Available since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

36. Stat3 pcDNA3

    Plasmid

    #8706

    Purpose

    See Depositor Comments below for variant information.

    Depositor

    Jim Darnell

    Article

    Zhong et al Proc Natl Acad Sci U S A 1994 May 24;91(

    Insert

    Stat3 (Stat3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    * see comments

    Promoter

    Available since

    Sept. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pOPS0379

    Plasmid

    #115510

    Purpose

    Suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing sfGFP

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    Rlv3841pNifH(pOGG082) sfGFP (pOGG037) and T-pharma (pOGG003) assembled in pOGG026
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Puro-iNgn3

    Plasmid

    #75337

    Purpose

    doxycycline inducible Ngn3 over-expression

    Depositor

    Danwei Huangfu

    Article

    Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-

    Insert

    neurogenin 3 (Neurog3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-tight

    Available since

    May 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pUltra

    Plasmid

    #24129

    Purpose

    3rd generation Lentiviral vector for bi-cistronic expression of EGFP and the gene of interest

    Depositor

    Malcolm Moore

    Article

    Lou et al PLoS One. 2012;7(3):e33093. doi: 10.1371

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pDESTpK7WG2mito

    Plasmid

    #158726

    Purpose

    Plant mitoTALEN vector assembly, Destination vector

    Depositor

    Shin-ichi Arimura

    Article

    Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

    Insert

    mitochondrial presequence in front of attR1 in pk7WG2
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge