Skip to main content
Addgene

We narrowed to 8,862 results for: BLI;

Showing: 6651 - 6700 of 8862 results
  1. SDC229-B03

    Plasmid
    #58763
    Purpose
    bacterial expression of PDB 4PYZ
    Depositor
    Insert
    PDB:4PYZ (USP7 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Bacterial expression for structure determination;…
    Promoter
    T7
    Available since
    Aug. 5, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
  2. pLKO-Tet-On-HDAC1-shRNA3

    Plasmid
    #90243
    Purpose
    Lentivirus for expression of shRNA3 against human HDAC1 (Dox-inducible)
    Depositor
    Insert
    HDAC1 (HDAC1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 17, 2017
    Availability
    Academic Institutions and Nonprofits only
  3. Myog'-2A-mCherryNLS-PGK-Puro

    Plasmid
    #69548
    Purpose
    Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.
    Depositor
    Inserts
    2A-mCherry-NLS
    Myogenin 5' homology arm
    Myogenin 3' homology arm (Myog Mouse)
    Use
    Mouse Targeting
    Tags
    2x nuclear localization signal and PVT1-2A "…
    Expression
    Mutation
    Silent V205V mutation in myogenin gene to prevent…
    Promoter
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
  4. ataxin3:1-431_Q79_BV

    Plasmid
    #209733
    Purpose
    Bacterial expression for structure determination
    Depositor
    Insert
    Ataxin 3 (ATXN3 Human)
    Use
    Tags
    6xHis-SBP-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Polyhedrin
    Available since
    Nov. 13, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
  5. MS2_SC2_S_Omicron

    Plasmid
    #180229
    Purpose
    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Omicron SARS-CoV-2 S gene (Amino Acid 319-869)
    Depositor
    Inserts
    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Omicron (Amino Acid 319-869)
    Promoter
    T7
    Available since
    Jan. 28, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
  6. MS2_SC2_S_WT

    Plasmid
    #175044
    Purpose
    Generation of MS2 Virus-Like Particles packaged with the sequence for the WT SARS-CoV-2 S gene (Amino Acid 319-869)
    Depositor
    Inserts
    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    WT (Amino Acid 319-869)
    Promoter
    T7
    Available since
    Oct. 18, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
  7. AKT3

    Plasmid
    #39038
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    AKT3 (AKT3 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
  8. Syn-ChR-tdTomato

    Plasmid
    #99843
    Purpose
    AAV expression of ChR2(H134R)-tdTomato under the Syn promoter
    Depositor
    Insert
    ChR2(H134R)-tdTomato
    Use
    AAV
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    ChR2(H134R), carrying a single point mutation at …
    Promoter
    human synapsin I promoter
    Available since
    Jan. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
  9. human KANK1 Ankyrin Repeats pET151

    Plasmid
    #164053
    Purpose
    Expresses human KANK1 Ank domain in bacterial cells
    Depositor
    Insert
    Kidney Ankyrin Repeat-Containing Protein 1 (KANK1 Human)
    Use
    Tags
    His-tag, TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
  10. CYP7A1 (3SN5)

    Plasmid
    #32874
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    CYP7A1 (CYP7A1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    amino acids 25-503 only
    Promoter
    Available since
    Jan. 13, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
  11. WDR5

    Plasmid
    #36900
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    WDR5 (WDR5 Human)
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    mutation version of plasmid #60121 3UR4
    Promoter
    Available since
    Aug. 8, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
  12. pUASp YFP Rac1 CA

    Plasmid
    #52235
    Purpose
    Drosophila expression of YFP-Rac1 constitutively active mutant
    Depositor
    Insert
    Rac1 (Rac1 Fly)
    Use
    Drosophila
    Tags
    YFP
    Expression
    Insect
    Mutation
    Q61L; CA mutation
    Promoter
    GAL4
    Available since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only
  13. Hs.KRAS4a Q61L

    Plasmid
    #83171
    Purpose
    Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), Q61L mutation
    Depositor
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Q61L
    Promoter
    Available since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
  14. LMNB2

    Plasmid
    #36896
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    LMNB2 (LMNB2 Human)
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    contains Laminb2 Ig-like domain, amino acid resid…
    Promoter
    Available since
    June 19, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
  15. pPNT6-eGFP

    Plasmid
    #71303
    Purpose
    This vector can be used for homologous recombination and allows to obtain recombinant neomycin resistant fluorescent cells that can loose fluorescence after Cre expression
    Type
    Empty backbone
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 28, 2016
    Availability
    Academic Institutions and Nonprofits only
  16. MAT2B

    Plasmid
    #39080
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    MAT2B (MAT2B Human)
    Use
    Tags
    Tev His Flag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
  17. Hs.KRAS4b A146T

    Plasmid
    #83150
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), A146T mutation
    Depositor
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    A146T
    Promoter
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
  18. pCMV-T7-SpG-P2A-mScarlet (MNW006)

    Plasmid
    #174136
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Insert
    human codon optimized SpG with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R
    Promoter
    CMV and T7
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
  19. pLPS-hAkt1-gfp

    Plasmid
    #49388
    Purpose
    mammalian expression of human Akt1 fused to EGFP
    Depositor
    Insert
    Akt1 (AKT1 Human)
    Use
    Cre/Lox; Acceptor vector
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    tga to cca at nt 1725 with respect to GenBank ID …
    Promoter
    CMV
    Available since
    Nov. 21, 2013
    Availability
    Academic Institutions and Nonprofits only
  20. DHRS4

    Plasmid
    #39071
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    DHRS4 (DHRS4 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
  21. Hs.KRAS4a Q61R

    Plasmid
    #83172
    Purpose
    Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), Q61R mutation
    Depositor
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Q61R
    Promoter
    Available since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
  22. ASPH

    Plasmid
    #39094
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    ASPH (ASPH Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
  23. pTXB1-CRP/CAP

    Plasmid
    #51563
    Purpose
    Expression of E. coli CRP/CAP for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone
    Insert
    CRP/CAP
    Use
    Tags
    Intein-Chiting Binding Domanin
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 25, 2014
    Availability
    Academic Institutions and Nonprofits only
  24. NLuc CMV-Neo Flexi Vector pFC32K SARS-CoV2 5'Nsp1

    Plasmid
    #163319
    Purpose
    Encodes for first COVID protein - NSP1 fused to nano-luciferase
    Depositor
    Insert
    Nsp1 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Luciferase
    Tags
    NanoLuc
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
  25. Myc-gamma3 (in pCDNA3.1)

    Plasmid
    #118041
    Purpose
    GABAA receptor expression (gamma3 subunit) Myc-tag near N-terminus
    Depositor
    Insert
    GABRG3 (Gabrg3 Rat)
    Use
    Tags
    cMyc epitope EQKLISEEDL inserted between fourth a…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
  26. Myc-gamma1 (in pCDNA3.1)

    Plasmid
    #118038
    Purpose
    GABAA receptor expression (gamma1 subunit) Myc-tag near N-terminus
    Depositor
    Insert
    GABRG1 (Gabrg1 Rat)
    Use
    Tags
    cMyc epitope EQKLISEEDL inserted between fourth a…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
  27. Myc-gamma3 (in pCAGGS)

    Plasmid
    #118042
    Purpose
    GABAA receptor expression (gamma3 subunit) Myc-tag near N-terminus
    Depositor
    Insert
    GABRG3 (Gabrg3 Rat)
    Use
    Tags
    cMyc epitope EQKLISEEDL inserted between fourth a…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
  28. pEF1-puro/hpb1(short)

    Plasmid
    #59973
    Purpose
    integrin b1 short headpiece expression (beta-I, hybrid, PSI domains) expression
    Depositor
    Insert
    human integrin b1 (short) (ITGB1 Human)
    Use
    Tags
    Base coiled-coil and His-tag
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a promoter
    Available since
    Sept. 24, 2014
    Availability
    Academic Institutions and Nonprofits only
  29. Hs.KRAS4b G12A

    Plasmid
    #83142
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12A mutation
    Depositor
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    G12A
    Promoter
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
  30. ANKRA2

    Plasmid
    #36904
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    ANKRA2 (ANKRA2 Human)
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    amino acid residues 148-313 (ANK repeats)
    Promoter
    Available since
    June 19, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
  31. MS2_SC2_S_Lambda

    Plasmid
    #175048
    Purpose
    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Lambda SARS-CoV-2 S gene (Amino Acid 319-869)
    Depositor
    Inserts
    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Lambda (Amino Acid 319-869)
    Promoter
    T7
    Available since
    Oct. 18, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
  32. pJK371

    Plasmid
    #73596
    Purpose
    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-3) with C-terminal c-myc epitope
    Depositor
    Inserts
    delta-12 fatty acid acetylenase
    delta-12 fatty acid desaturase (form 3)
    Use
    Tags
    FLAG tag and c-myc tag
    Expression
    Yeast
    Mutation
    Point mutants in FAD2-3 ORF relative to deposited…
    Promoter
    GAL1 and GAL10
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
  33. pMT-DREDD-V5-His

    Plasmid
    #63749
    Purpose
    Expresses drosophila DREDD tagged with V5 and 6xHis epitopes, under control of metallothionein promoter
    Depositor
    Insert
    Death related ced-3/Nedd2-like caspase isoform E (Dredd Fly)
    Use
    Tags
    V5-6XHis
    Expression
    Insect
    Mutation
    Promoter
    pMT
    Available since
    April 7, 2015
    Availability
    Academic Institutions and Nonprofits only
  34. pTRIPZ (M)-HT-Cbx7-ATLm

    Plasmid
    #82522
    Purpose
    Lentivirus vector. Expresses mutant Halotag-Cbx7-ATLm fusion proteins in mammalian cells. Cbx7 ATL mutation of RKR70-72, K75, and KR77-78 to AGA70-72, A75, and AG77-78.
    Depositor
    Insert
    Chromobox Homolog 7 (CBX7 Human)
    Use
    Lentiviral
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    mutation of RKR70-72, K75, and KR77-78 to AGA70-7…
    Promoter
    CMV
    Available since
    Dec. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
  35. BMPR2

    Plasmid
    #38972
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    BMPR2 (BMPR2 Human)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
  36. HOXC6 (human) HIS-tag pET

    Plasmid
    #8565
    Depositor
    Insert
    HOXC6 (HOXC6 Human)
    Use
    Tags
    His and T7
    Expression
    Bacterial
    Mutation
    This protein starts at an internal Met initially …
    Promoter
    Available since
    May 30, 2006
    Availability
    Academic Institutions and Nonprofits only
  37. pUASp YFP Rho1 DN

    Plasmid
    #52231
    Purpose
    Drosophila expression of YFP-Rho1 dominant negative mutant
    Depositor
    Insert
    Rho1 (Rho1 Fly)
    Use
    Drosophila
    Tags
    YFP
    Expression
    Insect
    Mutation
    T19N; DN mutation
    Promoter
    GAL4
    Available since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only
  38. MS2_SC2_S_Alpha

    Plasmid
    #175045
    Purpose
    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Alpha SARS-CoV-2 S gene (Amino Acid 319-869)
    Depositor
    Inserts
    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Alpha (Amino Acid 319-869)
    Promoter
    T7
    Available since
    Oct. 18, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
  39. MS2_SC2_S_Beta

    Plasmid
    #175046
    Purpose
    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Beta SARS-CoV-2 S gene (Amino Acid 319-869)
    Depositor
    Inserts
    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Beta (Amino Acid 319-869)
    Promoter
    T7
    Available since
    Oct. 18, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
  40. pGEX2TK-YAP-WW1

    Plasmid
    #104305
    Purpose
    bacterial expression of WW domain 1 of YAP; binds PPxY where Y is not phosphorylated
    Depositor
    Insert
    YAP WW1 domain (YAP1 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    WW domain 1
    Promoter
    tac
    Available since
    Jan. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
  41. pAAV-CMV-FLEX-SaCas9-U6-sgKcnj6

    Plasmid
    #159913
    Purpose
    Mutagenesis of Kcnj6
    Depositor
    Insert
    Kcnj6 (Kcnj6 Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
  42. 4IA9

    Plasmid
    #51320
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    WDR5 (WDR5 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Truncation: See Comments
    Promoter
    T7
    Available since
    Feb. 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
  43. HECW2

    Plasmid
    #32875
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    HECW2 (HECW2 Human)
    Use
    Tags
    His tag (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
  44. 4PZI

    Plasmid
    #61955
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    MLL4:JMC054-D08:C226821 (KMT2B Human)
    Use
    Tags
    6xHis and MHHHHHHSSGRENLYFQG
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    March 2, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 6651 - 6700 of 8862 results