We narrowed to 8,360 results for: construct

1. pETDUET1-hUCH37 (ISF1) - hNFRKB (1-156)

   Plasmid

   #61939

   Purpose

   Co-expression of hUCH37 (ISF1) and hNFRKB (1-156) in E.coli

   Depositor

   Chris Hill

   Article

   VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

   Inserts

   hUCH37 (ISF1) (UCHL5 Human)
   hNFRKB (1-156)
   

   Use

   Tags

   6x HIS

   Expression

   Bacterial

   Mutation

   Construct ends at residue 156

   Promoter

   T7

   Available since

   Oct. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pMU103

   Plasmid

   #73934

   Purpose

   This is hpRNA construct for gene silencing in soybean seeds. Soybean seed specific promoter, Glycinin gene promoter, is used.

   Depositor

   Zhanyuan Zhang

   Article

   Flores et al Transgenic Res. 2008 Oct;17(5):839-50. d

   Insert

   bar and GUS cassettes
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET151-GFP-PP-RPN2 (925-953)

   Plasmid

   #73744

   Purpose

   Expression of GFP-PP-RPN2 (925-953) fusion in E.coli

   Depositor

   Chris Hill

   Article

   VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

   Insert

   RPN2 (RPN2 Human)
   

   Use

   Tags

   6x His and GFP

   Expression

   Bacterial

   Mutation

   Construct possesses only residues 932-953 of RPN2

   Promoter

   T7

   Available since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

4. 8xCTS1_delta-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

   Plasmid

   #124545

   Purpose

   Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.

   Depositor

   Tudor Fulga

   Article

   Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

   Insert

   delta-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   d11-41, d11-41

   Promoter

   Minimal ADE

   Available since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMDJ13 - pEXP[Peft-3 | tagRFP-T (NLS) | tbb-2 UTR]

   Plasmid

   #159800

   Purpose

   Expression construct with ubiquitous expression to test fluorescence level on microscope

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   pEXP[Peft-3 | tagRFP-T (NLS) | tbb-2 UTR]
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pWJPrP1

   Plasmid

   #22109

   Depositor

   Susan Lindquist

   Article

   Jackson et al Neuron. 2009 Aug 27. 63(4):438-50.

   Insert

   mouse PrP ORF insert (Prnp Mouse)
   

   Use

   Construction of targeting vector

   Tags

   Expression

   Mutation

   mutations at aa 108 and 111 to make 3F4 epitope (…

   Promoter

   Available since

   Oct. 16, 2009

   Availability

   Academic Institutions and Nonprofits only

7. t2. pT7-7-SNCA-5Lys

   Plasmid

   #107294

   Purpose

   Plasmid for the expression of the construct SNCA-5Lys. Alpha-Synuclein with 12 aa C-terminal moiety, which consists of 5 Lys and 7 Gly

   Depositor

   Dmytro Yushchenko

   Article

   Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

   Insert

   SNCA-5Lys (SNCA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. r. pT7-7-SNCA-5Asn

   Plasmid

   #107295

   Purpose

   Plasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 12 aa C-terminal moiety, which consists of 5 Asn and 7 Gly

   Depositor

   Dmytro Yushchenko

   Article

   Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

   Insert

   SNCA-5Asn (SNCA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. s2. pT7-7-SNCA-5Asp

   Plasmid

   #107293

   Purpose

   Plasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 12 aa C-terminal moiety, which consists of 5 Asp and 7 Gly

   Depositor

   Dmytro Yushchenko

   Article

   Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

   Insert

   SNCA-5Asp (SNCA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET151-GFP-PP-RPN2 (940-953)

    Plasmid

    #73746

    Purpose

    Expression of GFP-PP-RPN2 (940-953) fusion in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

    Insert

    RPN2 (RPN2 Human)
    

    Use

    Tags

    6x His and GFP

    Expression

    Bacterial

    Mutation

    Construct possesses only residues 940-953 of RPN2

    Promoter

    T7

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

11. pET151-GFP-PP-RPN2 (918-932)

    Plasmid

    #73748

    Purpose

    Expression of GFP-PP-RPN2 (918-932) fusion in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

    Insert

    RPN2 (RPN2 Human)
    

    Use

    Tags

    6x His and GFP

    Expression

    Bacterial

    Mutation

    Construct possesses only residues 918-932 of RPN2

    Promoter

    T7

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

12. pLG1-puro-sgATL2-2

    Plasmid

    #109009

    Purpose

    CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    ATL2-sgRNA #2 (ATL2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 promoter

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCDNA3.1(-) + SLNCR1 _462-572 (conserved sequence)

    Plasmid

    #86818

    Purpose

    mammalian expression of lncRNA LINC00673/SLNCR1 deletion construct

    Depositor

    Carl Novina

    Article

    Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

    Insert

    LINC00673 (LINC00673 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pWJPrP5

    Plasmid

    #22106

    Depositor

    Susan Lindquist

    Article

    Jackson et al Neuron. 2009 Aug 27. 63(4):438-50.

    Insert

    mouse PrP ORF insert (Prnp Mouse)
    

    Use

    Construction of targeting vector

    Tags

    Expression

    Mutation

    mutated codon 177 (D to N)

    Promoter

    Available since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

15. FLAG-SpEAF 60-251 pBacPAK8

    Plasmid

    #15321

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Banks et al J Biol Chem. 2007 Feb 23. 282(8):5761-9.

    Insert

    EAF1 (eaf1 Fission Yeast)
    

    Use

    Baculovirus construction

    Tags

    FLAG

    Expression

    Mutation

    N terminal deletion lacking first 59 amino acids …

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

16. FLAG-ELL 1-373 pBacPAK8

    Plasmid

    #15328

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Kong et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

    Insert

    eleven nineteen lysine rich in leukemia (ELL Human)
    

    Use

    Baculovirus construction

    Tags

    FLAG

    Expression

    Mutation

    Amino acids 1-373

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

17. FLAG-ELL 45-621 pBacPAK8

    Plasmid

    #15329

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Kong et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

    Insert

    eleven nineteen lysine rich in leukemia (ELL Human)
    

    Use

    Baculovirus construction

    Tags

    FLAG

    Expression

    Mutation

    Amino acids 45-621

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

18. FLAG-ELL 45-373 pBacPAK8

    Plasmid

    #15330

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Kong et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

    Insert

    eleven nineteen lysine rich in leukemia (ELL Human)
    

    Use

    Baculovirus construction

    Tags

    FLAG

    Expression

    Mutation

    Amino acids 45-373

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

19. FLAG ELL 500-621 pBacPAK8

    Plasmid

    #15331

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Kong et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

    Insert

    eleven nineteen lysine rich in leukemia (ELL Human)
    

    Use

    Baculovirus construction

    Tags

    FLAG

    Expression

    Mutation

    Amino acids 500-621 only

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pTetOFF SNCA-A53T

    Plasmid

    #215372

    Purpose

    All-in-one lentiviral construct for the dox repressible expression of the A53T mutant of alpha-synuclein.

    Depositor

    Franz-Ulrich Hartl

    Insert

    SNCA (SNCA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Ala53Thr

    Promoter

    Available since

    Aug. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pJV505

    Plasmid

    #180078

    Purpose

    MS.SenLT2II_L85Q expression construct to improve transformation in S. enterica LT2

    Depositor

    Chase Beisel

    Article

    Vento et al Mol Cell. 2024 Jul 25;84(14):2785-2796.e

    Insert

    M.SenLT2II_L85Q
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P70a

    Available since

    Aug. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pJV303

    Plasmid

    #180079

    Purpose

    M.SenLT2I expression construct to improve transformation in S. enterica LT2

    Depositor

    Chase Beisel

    Article

    Vento et al Mol Cell. 2024 Jul 25;84(14):2785-2796.e

    Insert

    M.SenLT2I
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P70a

    Available since

    Aug. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pJV457

    Plasmid

    #180080

    Purpose

    M.BbrUI expression construct to improve transformation in B. breve UCC2003

    Depositor

    Chase Beisel

    Article

    Vento et al Mol Cell. 2024 Jul 25;84(14):2785-2796.e

    Insert

    M.BbrUI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P70a

    Available since

    Aug. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJV461

    Plasmid

    #180081

    Purpose

    M.BbrUIII expression construct to improve transformation in B. breve UCC2003

    Depositor

    Chase Beisel

    Article

    Vento et al Mol Cell. 2024 Jul 25;84(14):2785-2796.e

    Insert

    M.BbrUIII
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P70a

    Available since

    Aug. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGTPA-lb

    Plasmid

    #171528

    Purpose

    Plasmid contains the Cas12k gRNA for mutant library construction

    Depositor

    Quanjiang Ji

    Article

    Chen et al Cell Rep. 2021 Aug 31;36(9):109635. doi:

    Insert

    Cas12k gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Constitutive promoter J23119

    Available since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. p-HITI-GFP-Ckm

    Plasmid

    #226119

    Purpose

    HITI insert construct with Ckm gRNA and GFP transgene

    Depositor

    Christopher Nelson

    Article

    Padmaswari et al Mol Ther Nucleic Acids. 2024 Sep 2;35(4)

    Insert

    Ckm (Ckm Mouse)
    

    Use

    CRISPR

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. rAAV-Ef1α-EGFP-WPRE-ID-PolyA

    Plasmid

    #241829

    Purpose

    AAV construct for constitutive expression of EGFP. Contains a molecular barcode for identification of AAV genome using single-cell sequencing.

    Depositor

    Benjamin Arenkiel

    Article

    Belfort et al Front Neurosci. 2025 Feb 13;19:1531122.

    Insert

    EGFP-WPRE-ID-PolyA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. EGFP-MYO10-BioID

    Plasmid

    #145137

    Purpose

    Expresses EGFP-MYO10-BioID construct in mammalian cells

    Depositor

    Johanna Ivaska

    Article

    Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

    Insert

    MYO10-BioID (MYO10 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. mScarlet-I-Myo10 ΔF

    Plasmid

    #145139

    Purpose

    Expresses mScarlet-I tagged MYO10 construct with a MyTH/FERM domain deletion

    Depositor

    Johanna Ivaska

    Article

    Miihkinen et al Cell Rep. 2021 Sep 14;36(11):109716. doi

    Insert

    MYO10 ΔF (MYO10 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pXYB156

    Plasmid

    #239632

    Purpose

    Plant RNA Vision constructs with with 82 bp gRNAs targeting Tobacco rattle virus (TRV)

    Depositor

    Xiaohan Yang

    Article

    Liu et al Plant Biotechnol J. 2025 May;23(5):1640-

    Inserts

    sfGFPn_Y66-P1_loop_01-stem01-gRNA15
    gRNA16-stem_02-P1_loop_03-internal guide sequence-Ribozyme-sfGFPn_Y66
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pXYB085

    Plasmid

    #239637

    Purpose

    Plant RNA Vision constructs with 164 bp gRNAs together with RFP input

    Depositor

    Xiaohan Yang

    Article

    Liu et al Plant Biotechnol J. 2025 May;23(5):1640-

    Inserts

    sfGFPn_Y66-P1_loop_01-stem01-gRNA09
    gRNA10-stem_02-P1_loop_03-internal guide sequence-Ribozyme-sfGFPn_Y66
    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pXYB147

    Plasmid

    #239629

    Purpose

    Plant RNA Vision constructs with with 82 bp gRNAs targeting AT3G20840

    Depositor

    Xiaohan Yang

    Article

    Liu et al Plant Biotechnol J. 2025 May;23(5):1640-

    Inserts

    sfGFPn_Y66-P1_loop_01-stem01-gRNA05
    gRNA06-stem_02-P1_loop_03-internal guide sequence-Ribozyme-sfGFPn_Y66
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pXYB084

    Plasmid

    #239636

    Purpose

    Plant RNA Vision constructs with 123 bp gRNAs together with RFP input

    Depositor

    Xiaohan Yang

    Article

    Liu et al Plant Biotechnol J. 2025 May;23(5):1640-

    Inserts

    sfGFPn_Y66-P1_loop_01-stem01-gRNA07
    gRNA08-stem_02-P1_loop_03-internal guide sequence-Ribozyme-sfGFPn_Y66
    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pXYB086

    Plasmid

    #239638

    Purpose

    Plant RNA Vision constructs with 325 bp gRNAs together with RFP input

    Depositor

    Xiaohan Yang

    Article

    Liu et al Plant Biotechnol J. 2025 May;23(5):1640-

    Inserts

    sfGFPn_Y66-P1_loop_01-stem01-gRNA11
    gRNA12-stem_02-P1_loop_03-internal guide sequence-Ribozyme-sfGFPn_Y66
    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pXYB155

    Plasmid

    #239631

    Purpose

    Plant RNA Vision constructs with with 82 bp gRNAs targeting Tobacco rattle virus (TRV)

    Depositor

    Xiaohan Yang

    Article

    Liu et al Plant Biotechnol J. 2025 May;23(5):1640-

    Inserts

    sfGFPn_Y66-P1_loop_01-stem01-gRNA13
    gRNA14-stem_02-P1_loop_03-internal guide sequence-Ribozyme-sfGFPn_Y66
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pVTCL2

    Plasmid

    #234920

    Purpose

    All-in-one subcellar localization vector. Construct containing EGFP tag and plasma membrane-targeted mCherry for co-localization study in plants.

    Depositor

    Guoli Song

    Article

    Hao et al Plant Commun. 2025 May 19:101379. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pVTCL4

    Plasmid

    #234922

    Purpose

    All-in-one subcellar localization vector. Construct containing EGFP tag and Golgi-targeted mCherry for co-localization study in plants.

    Depositor

    Guoli Song

    Article

    Hao et al Plant Commun. 2025 May 19:101379. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pVTCL3

    Plasmid

    #234921

    Purpose

    All-in-one subcellar localization vector. Construct containing EGFP tag and cell endoplasmic reticulum-targeted mCherry for co-localization study in plants.

    Depositor

    Guoli Song

    Article

    Hao et al Plant Commun. 2025 May 19:101379. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMRX-IP-EGFP-LOV2-OMP25

    Plasmid

    #233346

    Purpose

    Stable expression of EGFP-LOV2(N538E)-OMP25 in mammalian cells. This construct lacks BAX domain, and causes no rupture upon photostimulation.

    Depositor

    Noboru Mizushima

    Article

    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

    Inserts

    LOV2 domain of phototropin1
    Omp25 (Synj2bp Rat)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N-terminus is deleted and N538E

    Promoter

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pSCT376: pTwinStrep-bdSUMO-ZooTsy_TnsF-Y584F

    Plasmid

    #200877

    Purpose

    ZooTsy_TnsF expression construct for protein purification.

    Depositor

    Feng Zhang

    Article

    Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

    Insert

    ZooTsy_TnsF
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    TnsF-Y584F

    Promoter

    Available since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pSCT162: pTwinStrep-bdSUMO-AjTn6022_TnsF

    Plasmid

    #200865

    Purpose

    AjTn6022_TniQ expression construct for protein purification.

    Depositor

    Feng Zhang

    Article

    Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

    Insert

    AjTn6022_TnsF
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCas3-APOBEC1

    Plasmid

    #229536

    Purpose

    pMV_hyg encoding Cas3(wt)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

    Depositor

    Kevin Verstrepen

    Article

    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

    Inserts

    rAPOBEC1
    Cas3
    Uracil glycosylase inhibitor
    

    Use

    Tags

    XTEN

    Expression

    Bacterial and Yeast

    Mutation

    codon optimized for S.cerevisiae and codon optimi…

    Promoter

    Available since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCas3-CDA1

    Plasmid

    #229534

    Purpose

    pMV_hyg encoding Cas3(wt)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

    Depositor

    Kevin Verstrepen

    Article

    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

    Inserts

    Cas3
    Cytidine deaminase
    Uracil glycosylase inhibitor
    

    Use

    Tags

    XTEN

    Expression

    Bacterial and Yeast

    Mutation

    codon optimized for S.cerevisiae and codon optimi…

    Promoter

    Available since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pR-mOrange2

    Plasmid

    #228608

    Purpose

    Mammalian expression of mOrange2, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

    Insert

    mOrange2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    We introduced some silent mutations to avoid esse…

    Promoter

    CMV

    Available since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pR-mBeRFP

    Plasmid

    #228601

    Purpose

    Mammalian expression of mBeRFP, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

    Insert

    mBeRFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    We introduced some silent mutations to avoid esse…

    Promoter

    CMV

    Available since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pR-mTagBFP2

    Plasmid

    #228596

    Purpose

    Mammalian expression of mTagBFP2, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

    Insert

    mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    We introduced some silent mutations to avoid esse…

    Promoter

    CMV

    Available since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pR-mT-Sapphire

    Plasmid

    #228597

    Purpose

    Mammalian expression of mT-Sapphire, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

    Insert

    mT-Sapphire
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pR-LSSmOrange

    Plasmid

    #228600

    Purpose

    Mammalian expression of LSSmOrange, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

    Insert

    LSSmOrange
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    We introduced some silent mutations to avoid esse…

    Promoter

    CMV

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pR-mTFP1

    Plasmid

    #228602

    Purpose

    Mammalian expression of mTFP1, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

    Insert

    mTFP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    We introduced some silent mutations to avoid esse…

    Promoter

    CMV

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pR-mRuby3

    Plasmid

    #228609

    Purpose

    Mammalian expression of mRuby3, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

    Insert

    mRuby3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    We introduced some silent mutations to avoid esse…

    Promoter

    CMV

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge