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psiCheck-DNAJA4CDS

Plasmid

#41849

Depositor

Sohail Tavazoie

Article

Pencheva et al Cell. 2012 Nov 21;151(5):1068-82. doi: 1

Insert

DNAJA4 CDS (DNAJA4 Human)

Use

Luciferase

Tags

renilla luciferase

Mutation

Start codon of DNAJA4 removed

Available Since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only
pBT250_(pCA-G-intron(Neo)-tTA2)

Plasmid

#36876

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP
tTA2
beta-globin intron
Neo

Expression

Mammalian

Mutation

deleted nucleotides after nucleotide 274, inserti…

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Available Since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/AUG-stop-nLuc-3XFLAG

Plasmid

#127308

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon, but translation terminated immediately by stop codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/CUG-nLuc-3XFLAG-CL1/PEST

Plasmid

#127316

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/ACG-nLuc-3XFLAG-CL1/PEST

Plasmid

#127318

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from ACG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pTV-Met-Eif4a1-tdiRFP670-PEST

Plasmid

#122371

Purpose

Targeting vector for mouse Eif4a1 to insert 2A-tdiRFP670-PEST-2A into immediately downstream of start codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST-2A with homology arms for Eif4a1

Use

Mouse Targeting

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCA-G-intron(Neo)-T(LNL)

Plasmid

#36907

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP
tdTomato-3Myc
beta-globin intron
Neo

Tags

3 Myc tags

Expression

Mammalian

Mutation

deleted nucleotides after nucleotide 274, inserti…

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Available Since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pETDUET1-hUCH37 (ISF1)-NFRKB(39-156)

Plasmid

#61943

Purpose

Co-expression of hUCH37 (ISF1) and hNFRKB (39-156) in E.coli

Depositor

Chris Hill

Article

VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

Inserts

hUCH37 (ISF1) (UCHL5 Human)
hNFRKB (1-156)

Tags

6x HIS

Expression

Bacterial

Mutation

Residues 2-39 deleted resulting in construct star…

Promoter

T7

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only
pENTR_HA-spGFP11

Plasmid

#240224

Purpose

Gateway entry clone for cloning in insert sequences by gibson assembly to create a C-terminal HA-spGFP11 tag. No ATG start codon.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv [Preprint]. 2025 Jul 11:2025.07.

Type

Empty backbone

Use

Gateway shuttle vector

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR4 HopU1

Plasmid

#228395

Purpose

pENTR4 plasmid with CDS of Pseudomonas syringae pv tomato type III effector HopU1 without stop codon for C-terminal epitope tags

Depositor

Lennart Wirthmueller

Article

Simranjit et al bioRxiv 2023.09.25.558804

Insert

HopU1

Use

Gateway-compatible entry vector

Mutation

Alanine inserted after start Methionine due to Nc…

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-TadA8e-Sauri-U6-Camk2d sgRNA1

Plasmid

#220117

Purpose

Expresses SauriABE8e by EFS promoter and sgRNA targeting murine Camk2d start codon site by U6 promoter

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

TadA8e, nSauriCas9

Use

AAV

Promoter

EFS

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-MYL2-TadA8e-Sauri-U6-Camk2d sgRNA1

Plasmid

#220120

Purpose

Expresses SauriABE8e by MYL2 promoter and sgRNA targeting murine Camk2d start codon site by U6 promoter

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

MYL2, TadA, nSauriCas9

Use

AAV

Promoter

MYL2

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pSG068

Plasmid

#214259

Purpose

pJN105 with PaftsH2SD and PaFtsH1 ORF cloned between NheI/XbaI

Depositor

Ute Romling

Article

Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi

Insert

Core genome PaFtsH1 proteinase from Pseudomonas aeruginosa SG17M

Expression

Bacterial

Mutation

18 nucleotide 5' of the start codon (Shine-D…

Promoter

araBAD promoter

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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AA195

Plasmid

#215984

Purpose

Fragmid fragment: (N' terminus) ALFA tag for recruiting via NbALFA

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; BPNLS_v1.1; ALFA_v1.3; ALFA_v1.2; ALFA_v1.1; ALFA_v1.4; ALFA_v1.5

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA198

Plasmid

#215985

Purpose

Fragmid fragment: (N' terminus) activator from p65 subunit of mouse Nfkb

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; BPNLS_v1.1; p65_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA125

Plasmid

#215982

Purpose

Fragmid fragment: (N' terminus) activator from p65 subunit of mouse Nfkb

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; p65_v1.3; HSF1_v1.2; Linker_v3.1; SV40NLS_v1.6

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA081

Plasmid

#215977

Purpose

Fragmid fragment: (N' terminus) HIV Nuclear Export Sequence

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; HIV-NES_v1.2; HIV-NES_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA124

Plasmid

#215981

Purpose

Fragmid fragment: (N' terminus) catalytic core of human acetyltransferase p300

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; p300_v1.2; Linker_v3.1; SV40NLS_v1.6

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pNHT7::UMSBP::sNluc2_ORF1

Plasmid

#186765

Purpose

An mRNA production vector encoding planarian codon-optimized sNluc2 with UMSBP 5' and 3' UTRs beginning at the endogenous gene's start codon.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Nanoluciferase

Promoter

T7

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pTL464

Plasmid

#69881

Purpose

a dCirl transcriptional reporter allele that contains an optimized gal4.2::p65 cassette at the start codon of the genomic dCirl ORF

Depositor

Robert Kittel, Tobias Langenhan

Article

Scholz et al Cell Rep. 2015 May 12;11(6):866-74. doi:

Insert

CIRL (Cirl Fly)

Use

Phic31-integration vector

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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