We narrowed to 27,291 results for: Tat

1. pLANT-2b

   Plasmid

   #231487

   Purpose

   Expresses His6-SUMO^NIT-FraB where "NIT" stands for "no internal translation", reflecting the mutation of the two Shine-Dalgarno-like sequences within the SUMO tag.

   Depositor

   Venkat Gopalan

   Inserts

   SUMO^NIT (SMT3 Budding Yeast)
   FraB
   

   Use

   Tags

   His6 tag and His6-SUMO^NIT

   Expression

   Bacterial

   Mutation

   Deleted amino acids 1-6 (MEPEES). and M83L, E84Q,…

   Promoter

   T7 promoter

   Available since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGG-PIP57

   Plasmid

   #196795

   Purpose

   Contains VSP2 promoter

   Depositor

   Marc Somssich

   Article

   Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

   Insert

   VEGETATIVE STORAGE PROTEIN 2 (VSP2 Mustard Weed)
   

   Use

   Greengate (gg) entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFastBac1-RiSxph

   Plasmid

   #193102

   Purpose

   Protein expression in insect cells

   Depositor

   Dan Minor

   Article

   Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

   Insert

   Ranitomeya imitator saxiphilin
   

   Use

   Tags

   3C-eGFP-His10

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEM-601[insert(+1) TA-rich]

   Plasmid

   #221196

   Purpose

   601 nucleosome positioning sequence with an additional base pair inserted 22 nt from the dyad on the TA-rich side of the 601

   Depositor

   Gregory Bowman

   Article

   Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

   Insert

   601[insert(+1)TA-rich]
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   601 has an additional mutation added 22 bp from d…

   Promoter

   Available since

   Aug. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST17-ScChd1[R126A/R130A]

   Plasmid

   #221197

   Purpose

   ScChd1-aa118-1274 chromatin remodeler with R126A and R130A mutations

   Depositor

   Gregory Bowman

   Article

   Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

   Insert

   ScChd1 (aa118-1274)
   

   Use

   Tags

   6xHis-tag (Precission protease cleavable)

   Expression

   Bacterial

   Mutation

   R126A, R130A

   Promoter

   T7

   Available since

   Aug. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLX-EF1a_NKX2-1_HD_160-223AA

   Plasmid

   #221489

   Purpose

   NKX2-1 N-terminal and C-terminal truncation (homeodomain only) mutation lentiviral overexpression

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Deletion of 1-159AA N-terminal and 224-371AA C-te…

   Promoter

   EF1a

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPB-MGT4-d2EGFP_CMV-mCherry

   Plasmid

   #213764

   Purpose

   Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.

   Depositor

   Gaetano Gargiulo

   Article

   Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

   Insert

   MGT#4-d2EGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB-CLGT3-d2EGFP_CMV-mCherry

   Plasmid

   #213766

   Purpose

   Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.

   Depositor

   Gaetano Gargiulo

   Article

   Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

   Insert

   CLGT#3-d2EGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMuLE (L1-L4) 6X PRE

   Plasmid

   #206168

   Purpose

   pMuLE L1-L4 entry plasmid containing 6 consensus PGR bindings sites upstream of a minimal 2YBTATA promoter

   Depositor

   Renee van Amerongen

   Article

   Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

   Insert

   6X PRE
   

   Use

   Gateway cloning entry clone

   Tags

   Expression

   Mutation

   Promoter

   2YBTATA

   Available since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMuLE (L1-L4) 2X PRE

    Plasmid

    #206166

    Purpose

    pMuLE L1-L4 entry plasmid containing 2 consensus PGR bindings sites upstream of a minimal 2YBTATA promoter

    Depositor

    Renee van Amerongen

    Article

    Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

    Insert

    2X PRE
    

    Use

    Gateway cloning entry clone

    Tags

    Expression

    Mutation

    Promoter

    2YBTATA

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT2/sh-FYN1-GFP_Seq_C

    Plasmid

    #201399

    Purpose

    Knockdown of FYN tyrosine kinase. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Article

    Comba et al Neuro Oncol. 2020 Jun 9;22(6):806-818. d

    Insert

    FYN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFB_ROSA26_SpCas9_gRNAs

    Plasmid

    #174703

    Purpose

    Plasmid containing separate SpCas9 gRNAs for targeted excision of inserted STOP Cassette upstream of reporter alleles found in Ai14 and Ai6 mice. ITRs flank the cassette for easy vector creation.

    Depositor

    Beverly L. Davidson

    Article

    Lang et al Nat Commun. 2019 Jul 30;10(1):3415. doi:

    Insert

    SpCas9 dual gRNAs
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. DDX41_p.L237F_p.P238T_EGFP

    Plasmid

    #175845

    Purpose

    Expresses DDX41 with mutation

    Depositor

    Evan Braunstein

    Article

    Li et al Haematologica. 2016 Jun;101(6):e228-31.

    Insert

    DDX41 (DDX41 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    c.711G>T, p.L237F and c.712C>A, p.P238T

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. Tol2-GgVCL/*TBM

    Plasmid

    #162790

    Purpose

    MUTATED_transfection of mammalian cells with Vcl mutated in Tankyrase Binding Motif -II (TBM-II) under a β-actin promoter, with IRES-GFP allowing identification of transfected cells

    Depositor

    Laura Lafon-Hughes

    Article

    et al PeerJ

    Insert

    Mutated Gallus gallus vinculin (G454V) (VCL Chicken)
    

    Use

    Tags

    IRES-GFP

    Expression

    Bacterial and Mammalian

    Mutation

    changed Gly 454 to Val

    Promoter

    beta actin

    Available since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. Dcp2 ΔH1 Δ5H

    Plasmid

    #163582

    Purpose

    express Dcp2 ΔH1 Δ5H at endogenous level in S.cerevisiae

    Depositor

    Michael Rosen

    Article

    Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

    Insert

    Dcp2 (DCP2 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    L255A; mutate 440-450, 489-500, 701-708, 827-831,…

    Promoter

    DCP2 promoter

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. Dcp2C Δ5H

    Plasmid

    #163581

    Purpose

    express Dcp2C Δ5H at endogenous level in S.cerevisiae

    Depositor

    Michael Rosen

    Article

    Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

    Insert

    Dcp2 (DCP2 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Truncation of aa1-300; mutate aa440-450, aa489-50…

    Promoter

    DCP2 promoter

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Donor-PIGAmut

    Plasmid

    #153548

    Purpose

    Used as a donor vector for the targeted knock-in of a mutation in PIGA exon 6

    Depositor

    Hiroyuki Konishi

    Article

    Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

    Insert

    mutant PIGA (a 1,991-bp fragment from intron 5 and exon 6 with a 3-bp mutation in exon 6)
    

    Use

    Donor plasmid

    Tags

    N/A

    Expression

    Mutation

    a 3-bp mutation in exon 6

    Promoter

    No promoter

    Available since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. SMT215

    Plasmid

    #134818

    Purpose

    SMT205 with DHFR-FLAG-tag, western blot

    Depositor

    Tanja Kortemme

    Article

    Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1

    Inserts

    Dihydrofolate reductase
    Thymidylate synthase
    

    Use

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    Silent mutations to eliminate internal restrictio…

    Promoter

    T7 and TET

    Available since

    Oct. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. SMT101

    Plasmid

    #134815

    Purpose

    Dual expression of DHFR and TYMS, in vivo assays

    Depositor

    Tanja Kortemme

    Article

    Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1

    Inserts

    Dihydrofolate reductase
    Thymidylate synthase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Silent mutations to eliminate internal restrictio…

    Promoter

    T7

    Available since

    Oct. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRS314-U6-G96C/A97C/U100C/U101C

    Plasmid

    #82328

    Purpose

    U6 telestem mutant (G96C/A97C/U100C/U101C) for in vivo studies

    Depositor

    Aaron Hoskins

    Article

    Rodgers et al Nucleic Acids Res. 2016 Dec 15;44(22):10

    Insert

    U6 RNA with G96C/A97C/U100C/U101C mutation (snR6 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    U6 telestem mutant (A34G/A35G/G96C/A97C/U100C/U10…

    Promoter

    Available since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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