We narrowed to 5,960 results for: actin
-
Plasmid#207753PurposeDonor template for Blast-2A-mScarlet insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748DepositorInsertACTB Homology Arms flanking a Blast-mScarlet Cassette (ACTB Human)
UseCRISPR; Donor templateExpressionMammalianAvailable SinceDec. 1, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-N-Puro-mNeon-ACTB
Plasmid#207752PurposeDonor template for Puro-2A-mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748DepositorInsertACTB Homology Arms flanking a Puro-mNeon Cassette (ACTB Human)
UseCRISPR; Donor templateExpressionMammalianAvailable SinceNov. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-AviMta3-3xFiBsd
Plasmid#140962Purposefull-length wt Avi-Mta3-3XFLAG-iBsd cDNA w/CAG promoter and Blasticidin resistance, cloned from mouse ES cellsDepositorInsertmetastasis associated 3 (Mta3 Synthetic, Mouse)
Tags3xFLAG, Avi, and TEVExpressionMammalianPromoterCAGAvailable SinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mEGFP-HO-G
Plasmid#164163PurposeExpresses mEGFP (monomeric EGFP with the A206K mutation) under the control of CAG promoter, codon-optimized for Homo sapiens, basic vector, multiclonig site at C terminalDepositorInsertmEGFP A236K
ExpressionMammalianPromoterCAGAvailable SinceFeb. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEX-Mac-GFP
Plasmid#58803PurposeAAV-mediated expression of Mac-GFP under the CAG promoter, in floxed/reversed (Cre-dependent) mannerDepositorInsertMac-GFP
UseAAV and Cre/LoxTagsGFPExpressionMammalianPromoterCAGAvailable SinceAug. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCBh_3x Flag-NLS-enOsCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA
Plasmid#197026PurposeVector encoding a human codon-optimized enOsCas12f1 driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and mCherry driven by CMV promoterDepositorInserthumanized enOsCas12f1
ExpressionMammalianMutationD52R / T132RPromoterCBh, CMV, hU6Available SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCBh_3x Flag-NLS-denOsCas12f1-NLS-VPR-NLS-mCherry_pA_phU6_gRNA scaffold-spacer_pCMV_BFP_pA
Plasmid#197031PurposeVector encoding a human codon-optimized dead enOsCas12f1 (denOsCas12f1)-VPR driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and BFP driven by CMV promoterDepositorInserthumanized denOsCas12f1-VPR
ExpressionMammalianMutationD52R / T132R / D228A / D406APromoterCBh, CMV, hU6Available SinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hNme2Cas9-NLS(SV40)-3xFLAG (KAC582)
Plasmid#134331PurposeCAG promoter expression plasmid for human codon optimized Nme2Cas9 nuclease with C-terminal NLS (SV40) and 3x FLAG tagDepositorInserthuman codon optimized Nme2Cas9
TagsNLS(SV40)-3xFLAGExpressionMammalianPromoterCAGAvailable SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
hAID-BE3 (pRZ352)
Plasmid#131316PurposeCAG promoter expression plasmid for hAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.DepositorInserthAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP
ExpressionMammalianPromoterCAGAvailable SinceOct. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-DDdCas9VPP300-T2A-GFP-IRES-Neo
Plasmid#102887PurposePiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core activator followed by T2A-EGFP as a reporter and IRES-Neo as selectionDepositorInsertDDdCas9VP192-P300-T2A-GFP-IRES-Neo
UseCRISPRExpressionMammalianMutationD10A, H840APromoterCAGAvailable SinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL3-sgSEC61B.N1-CAG-Cas9-T2A-mCherry-P2A-Puro
Plasmid#216251PurposeExpress Cas9 with CAG promoter to improve the expression in hES cells and sgSEC61B.N1 to tag endogenous SEC61B N-terminusDepositorAvailable SinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLQ10714
Plasmid#183959PurposeU6-crKlf4-CAG-hyperdCas12a-miniVPRDepositorInsertsHyperdCas12a
crRNA targeting Klf4
UseCRISPRTagsHAExpressionMammalianMutationD832A, D156R, E292R, D235R, D350RPromoterCAG and U6Available SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJRH056
Plasmid#171637PurposePlasmid containing a U6 promoter expressing a spyCas9 tdTom sgRNA298 and CAG promoter expressing mTagBFP2DepositorInsertsspyCas9 sgRNA-tdTomato
mTagBFP2
UseCRISPR and LentiviralExpressionMammalianPromoterCAG and U6Available SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMX-CAG-CD3-P2A-CD90.2
Plasmid#163336PurposeRetroviral vector to overexpress the murine CD3 (TCR) components CD3 gamma, CD delta, CD epsilon and TCR zeta and surface marker CD90.2, separated by T2; F2A; E2A; P2A respectively, under control of the CAG promoterDepositorInsertCD3
UseRetroviralExpressionMammalianPromoterCAGAvailable SinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hSt3Cas9-NLS(SV40)-3xFLAG (KAC426)
Plasmid#133788PurposeCAG promoter expression plasmid for human codon optimized St3Cas9 nuclease with C-terminal NLS (SV40)DepositorInserthuman codon optimized St3Cas9
TagsNLS(SV40)-3xFLAGExpressionMammalianMutationn/aPromoterCAGAvailable SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mTHC
Plasmid#112620Purposeexpression plasmid with CAG promoter driving multicistronic cassette H2BmTeal_p2A_HygroR_p2A_CreERt2DepositorInsertsTagsmTeal (mTFP1)ExpressionMammalianPromoterCAGAvailable SinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
Cbh_v5 AAV-saCBE N-terminal
Plasmid#137182PurposeAAV genome: expresses the N-terminal of S. aureus v5 AAV-CBE from the Cbh promoter, U6-sgRNADepositorInsertv5 AAV-saCBE N-terminal
UseAAVMutationCas9 D10APromoterCbhAvailable SinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
CAG-YAP-5SA
Plasmid#248046PurposeEncodes onstitutively active YAP1 under the control of the CAG promoter. The construct is flanked by Tol2 sites for transgenesis.DepositorInsertMyc-YAP-5SA (YAP1 Human)
ExpressionBacterialMutationS61A, S109A, S127A, S164A, S381A (phosphorylation…PromoterpCaggsAvailable SinceMay 29, 2026AvailabilityAcademic Institutions and Nonprofits only -
CAG-mKate2-NLS
Plasmid#248044PurposeEncodes nuclear mKate2 fluorescent protein, under control of the CAG promoter. The construct is flanked by Tol2 sites for transgenesis.DepositorInsertmKate2-NLS
ExpressionBacterialMutationmKate2 was derived from mKate with the following …PromoterCAGAvailable SinceMay 29, 2026AvailabilityAcademic Institutions and Nonprofits only -
CAG-YAP1∆TA
Plasmid#248048PurposeEncodes a YAP1 mutant lacking the transactivation domain, under the control of the CAG promoter. The construct is flanked by Tol2 sites for transgenesis.DepositorInsertYAP1 (YAP1 Human)
ExpressionBacterialMutationTransactivationdomain deleted (aa291-497)PromoterCaggsAvailable SinceMay 29, 2026AvailabilityAcademic Institutions and Nonprofits only