We narrowed to 5,630 results for: donor

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pCRISPR-HOT_tdTomato

Plasmid

#138567

Purpose

Universal Cleavable Donor Plasmid for tagging with tdTomato using NHEJ

Depositor

Hans Clevers

Article

Artegiani et al Nat Cell Biol. 2020 Mar;22(3):321-331. d

Insert

tdTomato

Use

Unspecified

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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mTq2-Calmodulin

Plasmid

#198200

Purpose

FRET donor: Calmodulin-2 tagged with N-terminal mTurquoise2

Depositor

Christian Wahl-Schott

Article

Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

Insert

Calmodulin 2 (Calm2 Rat)

Tags

mTurquoise2

Expression

Mammalian

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pJ204-loxP-EF1a-mCherry-NeoR-lox2272-BGHpA-siteA-HDR

Plasmid

#154826

Purpose

Landing pad for Cre/lox-based recombinase-mediated cassette exchange (RMCE) in CHO cells, donor plasmid for CRISPR/Cas9-mediated targeted integration in CHO cells (site A)

Depositor

Lise Marie Grav

Article

Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

Inserts

mCherry
NeoR
ZsGreen1-DR

Use

CRISPR and Cre/Lox

Tags

PEST

Expression

Mammalian

Promoter

CMV, EF1a, and SV40 early promoter

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro CAG FUCCI

Plasmid

#136934

Purpose

donor plasmid for constitutive FUCCI expression in human cells

Depositor

Xiaoping Bao

Article

Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.

Insert

Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)

Use

Synthetic Biology

Tags

Clover and mKO2

Expression

Mammalian

Promoter

CAG

Available Since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro-CAG-GCaMP6s

Plasmid

#120896

Purpose

Donor plasmid targeting human AAVS1 safe harbor locus to generate GCaMP6s gene knockin. Contains two homologous arms flanking T2A-Puro resistance gene and CAG-driven GCaMP6s gene.

Depositor

Xiaojun Lian

Article

Jiang et al iScience. 2018 Oct 12;9:27-35. doi: 10.1

Insert

GCaMP6s

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pBR_attB(C31)_FRT

Plasmid

#183761

Purpose

phiC31-specific donor plasmid for the cloning of payloads <20 kb

Depositor

Richard Davis

Article

Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-trGluc

Plasmid

#158959

Purpose

DNA donor plasmid containing repair template for Gaussia luciferase (Gluc) of bioluminescent repair reporter (BLRR).

Depositor

Charles P. Lai

Article

Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589

Insert

trGluc

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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p15_attB(bxb)_lox

Plasmid

#183764

Purpose

Bxb1-specific donor plasmid for the cloning of payloads (~10 - 50 kb)

Depositor

Richard Davis

Article

Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

Type

Empty backbone

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pORANGE Cacnb3-GFP KI

Plasmid

#139662

Purpose

Endogenous tagging of CaVβ3: C-terminal (amino acid position: W479)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pJ204-loxP-EF1a-tagBFP-HygR-lox2272-BGHpA-siteT9-HDR

Plasmid

#154827

Purpose

Landing pad for Cre/lox-based recombinase-mediated cassette exchange (RMCE) in CHO cells, donor plasmid for CRISPR/Cas9-mediated targeted integration in CHO cells (site T9)

Depositor

Lise Marie Grav

Article

Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

Inserts

TagBFP
HygR
ZsGreen1-DR

Use

CRISPR and Cre/Lox

Tags

PEST

Expression

Mammalian

Promoter

CMV, EF1a, and SV40 early promoter

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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Bxb1_jRCaMP1b

Plasmid

#183758

Purpose

Bxb1-specific donor plasmid for intergrating a transcription unit for expression of jRCaMP1b calcium reporter

Depositor

Richard Davis

Article

Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

Insert

jRCaMP1b

Tags

NES

Expression

Mammalian

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pMS_366

Plasmid

#182131

Purpose

expresses retron RNA, ec.86 Reverse Transcriptase, and CspRecT SSAP, to create a specific rifampicin resistance mutation

Depositor

George Church

Article

Schubert et al Proc Natl Acad Sci U S A. 2021 May 4;118

Inserts

retron RNA
ec.86 RT
CspRecT

Mutation

incorporated rifampicin-resistance-conferring don…

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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AAVS1-Pur-CAG-hM3Dq-mCherry

Plasmid

#80948

Purpose

human AAVS1 site targeting donor plasmid for knock-in hM3Dq-mCherry expression cassette driven by CAG promoter

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2016 Jun 2;18(6):817-26.

Insert

hM3Dq-mCherry

Tags

mcherry fusion

Expression

Mammalian

Promoter

CAG promoter

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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AAVS1-Pur-CAG-hM4Di-mCherry

Plasmid

#80947

Purpose

human AAVS1 site targeting donor plasmid for knock-in hM4Di-mCherry expression cassette driven by CAG promoter

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2016 Jun 2;18(6):817-26.

Insert

hM4Di-mCherry

Tags

mcherry fusion

Expression

Mammalian

Promoter

CAG promoter

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro CAG J591-scFV T-CAR

Plasmid

#171961

Purpose

Donor plasmid for J591 T-CAR expression in human cells

Depositor

Xiaoping Bao

Article

Harris et al J Immunol Regen Med. 2023 May;20:100074.

Insert

Synthetic J591-scFV CAR

Use

CRISPR, Synthetic Biology, and TALEN

Expression

Mammalian

Promoter

CAG

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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AAVS1-Blasticidin-CAG-Flpe-ERT2

Plasmid

#68461

Purpose

AAVS1 targeting donor plasmid with Flpe-ERT2 expression cassette and blasticidin selection gene

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Insert

mammalian-codon-optimized Flpe recombinase fused with ERT2

Use

Synthetic Biology

Tags

ERT2

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

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AAVS1_dCas9-XTEN-KRAB-p2A-mCherry

Plasmid

#222107

Purpose

Donor vector for CRISPRi integration at AAVS1 with an mCherry fluorophore

Depositor

Jonathan Weissman

Article

She et al Cell. 2023 Jul 6;186(14):2977-2994.e23.

Insert

CRISPRi-kox1(KRAB) (cas9 Human, S. pyogenes)

Use

AAV and CRISPR

Tags

HA and P2A-mCherry

Expression

Mammalian

Mutation

D10A, H840A

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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CMV-mEGFP-CREB

Plasmid

#137001

Purpose

mEGFP-CREB donor for G-CREB

Depositor

Ryohei Yasuda

Article

Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

Insert

CREB1 (Creb1 Rat)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro CAG PSMA nanobody T-CAR

Plasmid

#171962

Purpose

Donor plasmid for PSMA nanobody T-CAR expression in human cells

Depositor

Xiaoping Bao

Article

Harris et al J Immunol Regen Med. 2023 May;20:100074.

Insert

Synthetic anti-PSMA nanobody CAR

Use

CRISPR, Synthetic Biology, and TALEN

Expression

Mammalian

Promoter

CAG

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pT2Apuro-TRE-synPCB2.0

Plasmid

#139481

Purpose

Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

Depositor

Kazuhiro Aoki

Article

Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

Insert

MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Fnr-Fd-T7

Use

Tol2 transposon donor vector

Expression

Mammalian

Promoter

Tet responsive element

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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