Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 146,353 results for: ins;

Showing: 2661 - 2680 of 146353 results

  3. 8xNFAT-ZsG-muhCD4

    Plasmid
    #162745
    Purpose
    NFAT-driven ZsGreen-1 reporter gene with mutated human CD4 gene
    Depositor
    Maki Nakayama
    Article
    Landry et al Front Endocrinol (Lausanne). 2021 Mar 25
    Insert
    8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (CD4 Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Human CD4 gene with glutamine to tyrosine at posi…
    Promoter
    Available since
    April 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    162745-plasmid-map-sequence-id-331322

    Enlarge

  4. pR I-Ab beta Derp1-117

    Plasmid
    #113639
    Purpose
    Insect cell expression vector for I-Ab beta chain fused to Der p 1 117-127 antigenic peptide
    Depositor
    James Moon
    Article
    Moon et al Methods Mol Biol. 2018;1799:165-181. doi
    Insert
    I-Ab beta Derp1-117 (H2-Ab1 Mouse)
    Use
    Tags
    6x His tag, fused to Der p 1 (117-127) peptide vi…
    Expression
    Insect
    Mutation
    transmembrane and cytoplasmic domains truncated
    Promoter
    Metallothionein
    Available since
    Sept. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    113639-plasmid-map-sequence-id-221159

    Enlarge

  5. pUC19-U6-BsmBI_cassette-Nme/Nme2Cas9-sgRNA (KAC32)

    Plasmid
    #133794
    Purpose
    U6 promoter sgRNA entry vector used for all NmeCas9 or Nme2Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Amrani et al. Genome Biology 2018
    Depositor
    Joseph Bondy-Denomy, Benjamin Kleinstiver
    Article
    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do
    Insert
    NmeCas9 and Nme2Cas9 sgRNA entry vector
    Use
    Tags
    Expression
    Mammalian
    Mutation
    sgRNA architecture from Amrani et al. Genome Biol…
    Promoter
    U6
    Available since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    133794-plasmid-map-sequence-id-268076

    Enlarge

  7. pJW2171

    Plasmid
    #163095
    Purpose
    CRISPR repair template: add insertion site specific homology arms by PCR before insertion
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    30aa linker::mNeonGreen (dpi)::AID*::3xFLAG::10aa linker
    Use
    CRISPR
    Tags
    Expression
    Worm
    Mutation
    Silent mutations to remove piRNA sites
    Promoter
    Available since
    Jan. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    163095-plasmid-map-sequence-id-320590

    Enlarge

  8. pCMV-T7-SpCas9-VQR-P2A-EGFP (RTW3520)

    Plasmid
    #139990
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VQR(D1135V/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9-VQR with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    VQR=D1135V/R1335Q/T1337R
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139990-plasmid-map-sequence-id-275521

    Enlarge

  11. pCMV-T7-SpCas9-VRER-P2A-EGFP (RTW3160)

    Plasmid
    #139991
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRER(D1135V/G1218R/R1335E/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    VRER=D1135V/G1218R/R1335E/T1337R
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139991-plasmid-map-sequence-id-275523

    Enlarge

  12. pCMV-T7-SpCas9-VRQR-P2A-EGFP (RTW3161)

    Plasmid
    #139992
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9-VRQR with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    VRQR=D1135V/G1218R/R1335Q/T1337R
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139992-plasmid-map-sequence-id-275524

    Enlarge

  15. YET1000: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X4)

    Plasmid
    #104571
    Purpose
    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to four DmrA domains
    Depositor
    Keith Joung
    Article
    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n
    Insert
    human codon optimized ‘dead’ Cpf1 fused to four DmrA domains
    Use
    CRISPR
    Tags
    NLS-3xHA-3xFLAG-4xDmrA
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    CAG
    Available since
    Jan. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    104571-plasmid-map-sequence-id-201436

    Enlarge

  16. pJW2086

    Plasmid
    #154335
    Purpose
    Crispr repair template: add insertion site specific homology arms by PCR before insertion
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    30aa linker::GFP::AID*::3xFLAG::10aa linker
    Use
    CRISPR
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    154335-plasmid-map-sequence-id-308930

    Enlarge

  19. pJW2172

    Plasmid
    #163096
    Purpose
    CRISPR repair template: add insertion site specific homology arms by PCR before insertion
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    30aa linker::mNeonGreen (dpi)::3xFLAG::10aa linker
    Use
    CRISPR
    Tags
    Expression
    Worm
    Mutation
    Silent mutations to remove piRNA sites
    Promoter
    Available since
    Jan. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    163096-plasmid-map-sequence-id-320618

    Enlarge

Showing: 2661 - 2680 of 146353 results