We narrowed to 4,947 results for: mcs

1. pREP82xN_ccdB

   Plasmid

   #79026

   Purpose

   Gateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, ura4+ selection

   Depositor

   Pernilla Bjerling, Göran Akusjärvi

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   July 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pREP81xN_ccdB

   Plasmid

   #79025

   Purpose

   Gateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, LEU2 selection

   Depositor

   Pernilla Bjerling, Göran Akusjärvi

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   July 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR CMVie-Lifeact-mCardinal L1-R5

   Plasmid

   #84392

   Purpose

   Multisite Gateway entry vector for expression of mCardinal-tagged Lifeact in cells including neurons

   Depositor

   Ghassan Mouneimne

   Insert

   Lifeact
   

   Use

   Gateway

   Tags

   mCardinal

   Expression

   Mammalian

   Promoter

   CMV immediate early

   Available Since

   Jan. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pREP41xN_ccdB

   Plasmid

   #79023

   Purpose

   Gateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, LEU2 selection

   Depositor

   Pernilla Bjerling, Göran Akusjärvi

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Aug. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pREP42xN_ccdB

   Plasmid

   #79024

   Purpose

   Gateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, ura4+ selection

   Depositor

   Pernilla Bjerling, Göran Akusjärvi

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   July 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-GfABC1D-NLS-GFP-WPRE-synpA-L1-R2

   Plasmid

   #203541

   Purpose

   gRNA expression in astrocytes

   Depositor

   Ben Deverman

   Article

   Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

   Insert

   NLS-GFP, 2x U6-RNA
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1_FLAG-YAP1

   Plasmid

   #173001

   Purpose

   constitutive expression of FLAG-tagged YAP1

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   FLAG-YAP1 (YAP1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEvol-pAcFRS.2.t1

   Plasmid

   #73544

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

   Depositor

   Farren Isaacs

   Article

   Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

   Inserts

   pAcFRS.2.t1
   pAcFRS.2.t1
   

   Expression

   Bacterial

   Available Since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Neo_CAG-Cas9

   Plasmid

   #86698

   Purpose

   Cas9 expression vector for targeting to the AAVS1 locus

   Depositor

   Ludovic Vallier

   Article

   Bertero et al Development. 2016 Dec 1;143(23):4405-441

   Insert

   Cas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   pCag

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. HA-CDC25C

    Plasmid

    #229410

    Purpose

    Lentiviral or overpexression of cDNA

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    CDC25C (CDC25C Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTRIP-SFFV-mtagBFP-2A STING V155M

    Plasmid

    #102591

    Purpose

    Lentivector to express STING V155M and mTagBFP

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    V155M

    Promoter

    SFFV

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lenti-EFS-EGFP-T2A-Bsd

    Plasmid

    #193198

    Purpose

    Lentiviral vector expressing EGFP (driven by EFS promoter) and a blasticidin resistance marker

    Depositor

    Julien Sage

    Article

    Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

    Insert

    EGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEvol-pAzFRS.2.t1

    Plasmid

    #73546

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

    Depositor

    Farren Isaacs

    Article

    Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

    Inserts

    pAzFRS.2.t1
    pAzFRS.2.t1
    

    Expression

    Bacterial

    Available Since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMXs-IP-mCherry-TOSI

    Plasmid

    #172496

    Purpose

    mCherry-TOSI (TOr-Signal-Indicator) is a fluorescent reporter for mTORC1 signaling (monitoring PDCD4 degradation) which can be introduced to mammalian cells by retrovirus vector.

    Depositor

    David Scadden

    Article

    Oki et al Nat Commun. 2021 Jan 11;12(1):245. doi:

    Insert

    PDCD4 (Pdcd4 Mouse)
    

    Use

    Retroviral

    Tags

    Mcherry

    Expression

    Mammalian

    Promoter

    MLV LTR

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP-MYO7A-TAIL

    Plasmid

    #89585

    Purpose

    Expression plasmid for a positive control bait protein in NanoSPD 2.0 assays.

    Depositor

    Thomas Friedman

    Article

    Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

    Insert

    MYO7A (Myo7a Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Truncation encoding amino acids 870-2166 of MYO7A…

    Promoter

    CMV

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-MYO10-HMM-Nanotrap

    Plasmid

    #87255

    Purpose

    Expression plasmid to perform NanoSPD 2.0 assays in mammalian cells.

    Depositor

    Thomas Friedman

    Article

    Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

    Insert

    MYO10 (Myo10 Mouse)
    

    Tags

    GFP-nanotrap

    Expression

    Mammalian

    Mutation

    Myo10 is truncated to contain aa.1-941

    Promoter

    CMV

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-CAG-SV40NLSf-mScarlet-P2A-hLuc-WPRE

    Plasmid

    #203539

    Purpose

    AAV reporter genome plasmid

    Depositor

    Ben Deverman

    Article

    Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

    Insert

    NLS-mScarlet-2A-mScarlet
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. lentiTet-LDLR-mCherry BlastR

    Plasmid

    #186739

    Purpose

    Dox inducible LDLR coding sequence expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene

    Depositor

    Richard Sherwood

    Article

    Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

    Insert

    LDLR (LDLR Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMXs-IP-mVenus-TOSI

    Plasmid

    #172491

    Purpose

    mVenus-TOSI (TOr-Signal-Indicator) is a fluorescent reporter for mTORC1 signaling (monitoring PDCD4 degradation) which can be introduced to mammalian cells by retrovirus vector.

    Depositor

    David Scadden

    Article

    Oki et al Nat Commun. 2021 Jan 11;12(1):245. doi:

    Insert

    Pdcd4 (Pdcd4 Mouse)
    

    Use

    Retroviral

    Tags

    mVenus

    Expression

    Mammalian

    Promoter

    MLV LTR

    Available Since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC19/PLIN2 GFP-P2A-BFP

    Plasmid

    #202195

    Purpose

    knock-in donor vector of GFP-P2A-BFP to C-terminus of endogenous human PLIN2

    Depositor

    James Olzmann

    Article

    Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

    Insert

    PLIN2 (PLIN2 Human)
    

    Use

    Cloning vector

    Tags

    GFP-P2A-BFP knock-in

    Promoter

    lac

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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