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pPRIME-CMV-GFP-shNts2

Plasmid

#132714

Purpose

Encodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat Nts

Depositor

Robert Messing

Article

Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

Insert

shNts(2) (Nts Rat)

Use

Lentiviral

Promoter

CMV

Available Since

Oct. 25, 2019

Availability

Academic Institutions and Nonprofits only

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AAVS1-A2UCOE-EF1a-mCR1-F2A-neo-AAVS1

Plasmid

#205447

Purpose

human AAVS1-targeting plasmid for expression of mouse CR1

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

mCR1

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA Arf6 ActQ67L

Plasmid

#10835

Depositor

Thomas Roberts

Article

Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

Insert

Arf6 Q67L (ARF6 Human)

Tags

HA

Expression

Mammalian

Mutation

Q67L. Defective in GTP hydrolysis. Constitutive…

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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pROF051

Plasmid

#155328

Purpose

Constitutive expression of SRDX-EL222 in plants

Depositor

Matias Zurbriggen

Article

Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

Insert

PCaMV35S-SRDX-NLS-EL222-Tnos

Use

Synthetic Biology

Expression

Plant

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG PTEN

Plasmid

#78777

Purpose

To overexpress PTEN in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al Nat Commun. 2015 Jul 17;6:7769. doi: 10.

Insert

PTEN (PTEN Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pRosetta_v2

Plasmid

#136477

Purpose

Lentiviral GFP Reporter assay for determining appropriate dose of puromycin, blasticidin, and hygromycin

Depositor

John Doench

Article

DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

Insert

HygroR-T2A-BlastR-P2A-PuroR-F2A-EGFP

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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CFP-CD82

Plasmid

#1818

Depositor

Walther Mothes

Article

Sherer et al Traffic 2003 Nov;4(11):785-801.

Insert

CD82 (CD82 Human)

Tags

CFP

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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STR-KDEL_PGRN-SBP-mCherry

Plasmid

#180439

Purpose

Co-expresses (via IRES) streptavidin-KDEL and Progranulin-SBP-mCherry

Depositor

Shawn Ferguson

Article

Devireddy et al J Cell Biol. 2022 Feb 7;221(2):e20210404

Insert

Progranulin (GRN Human)

Tags

SBP-mCherry

Expression

Mammalian

Available Since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-TDP-43-Q331K

Plasmid

#133761

Purpose

Lentiviral expression of human TDP-43 Q331K disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed glutamine 331 to lysine.

Promoter

CMV

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pSpCas9-2A-Puro-TBK1-gRNA (PX459)

Plasmid

#221550

Purpose

Expresses Cas9 and gRNA for disruption of TBK1 gene in human cells

Depositor

Shawn Ferguson

Article

Talaia et al EMBO J. 2024 Sep;43(18):3948-3967. doi:

Insert

gRNA targeting human TBK1 (TBK1 Human)

Expression

Mammalian

Promoter

u6

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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xCas9(3.7)-BE4

Plasmid

#108381

Purpose

Mammalian expression vector for xCas9(3.7)-BE4

Depositor

David Liu

Article

Hu et al Nature. 2018 Feb 28. pii: nature26155. d

Insert

xCas9(3.7)-BE4

Tags

NLS

Expression

Mammalian

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA Arf6 DN T27N

Plasmid

#10831

Depositor

Thomas Roberts

Article

Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

Insert

Arf6 T27N (ARF6 Human)

Tags

HA

Expression

Mammalian

Mutation

T27N. Defective in GTP binding. Dominant negati…

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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WDR59 in pRK5

Plasmid

#154214

Purpose

Untagged WDR59 cDNA expression vector for transfections

Depositor

David Sabatini

Article

Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

Insert

WDR59 (WDR59 Human)

Tags

NONE

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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xCas9(3.7)-BE3

Plasmid

#108380

Purpose

Mammalian expression vector for xCas9(3.7)-BE3

Depositor

David Liu

Article

Hu et al Nature. 2018 Feb 28. pii: nature26155. d

Insert

xCas9(3.7)-BE3

Tags

NLS

Expression

Mammalian

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-wtTnsC

Plasmid

#234732

Purpose

Wild-type human-codon optimized PseTnsC in mammalian cells

Depositor

David Liu

Article

Witte et al Science. 2025 May 15;388(6748):eadt5199.

Insert

PseTnsC

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p-eGFP-beta 2 CP

Plasmid

#13298

Depositor

John Cooper

Article

Schafer et al J Cell Biol. 1998 Dec 28. 143(7):1919-30

Insert

capping protein beta 2 subunit (Capzb Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pLJM1-FLAG-metap2

Plasmid

#100510

Purpose

Expresses FLAG tagged metap2 in mammalian cells to make lentivirus

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

METAP2 (METAP2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pOPINB-OTUB1*

Plasmid

#65441

Purpose

Expresses activated OTUB1 in E. coli

Depositor

David Komander

Article

Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

Inserts

UbcH5b (UBE2D2 Human)
otubain-1 (OTUB1 )

Tags

His-3C

Expression

Bacterial

Mutation

Active site cysteine mutated to alanine

Promoter

T7

Available Since

July 17, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA PTEN

Plasmid

#78776

Purpose

To overexpress PTEN in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al Nat Commun. 2015 Jul 17;6:7769. doi: 10.

Insert

PTEN (PTEN Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pAIP hIL2 co

Plasmid

#90513

Purpose

Lentiviral vector for expression of human codon optimized IL2 with puromycin resistance.

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

IL2 (IL2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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