We narrowed to 6,467 results for: poly

1. LifeAct-scramble

   Plasmid

   #217499

   Purpose

   Expresses LifeAct-mScarlet-scramble to serve as control plasmid for LifeAct-MTED

   Depositor

   Erik Dent

   Article

   Holland et al Mol Biol Cell. 2024 Jun 1;35(6):mr3. doi

   Insert

   LifeAct-mScarlet-scramble
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. LentiV_Puro

   Plasmid

   #111886

   Purpose

   Lentiviral expression of cDNA with puromycin resistance gene

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EFS promoter

   Available since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. MAP2-GCaMP3

   Plasmid

   #43917

   Depositor

   John Gearhart

   Article

   Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

   Insert

   GCaMP3
   

   Use

   Lentiviral

   Tags

   6xHis, T7 epitope, and Xpress tag

   Expression

   Mammalian

   Mutation

   E247V in GCaMP3

   Promoter

   Human MAP2 promoter

   Available since

   May 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. AviTag-SpyCatcher

   Plasmid

   #72326

   Purpose

   Bacterial expression of SpyCatcher with a peptide tag enabling site-specific biotinylation.

   Depositor

   Mark Howarth

   Article

   Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

   Insert

   AviTag-SpyCatcher
   

   Use

   Tags

   N-term His6 tag and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. SpyLigase

   Plasmid

   #51722

   Purpose

   Plasmid for recombinant expression in bacteria of SpyLigase. The Howarth lab has created two improved peptide ligation enzymes. See depositor comments below!

   Depositor

   Mark Howarth

   Article

   Fierer et al Proc Natl Acad Sci U S A. 2014 Mar 17.

   Insert

   SpyLigase
   

   Use

   Tags

   His6 Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. hMSH2 Clone E3 (full-length)

   Plasmid

   #16453

   Depositor

   Bert Vogelstein

   Article

   Leach et al Cell. 1993 Dec 17. 75(6):1215-25.

   Insert

   MSH2 (MSH2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOPINS-UBE3C

   Plasmid

   #66711

   Purpose

   Expresses UBE3C HECT domain in E. coli

   Depositor

   David Komander

   Article

   Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

   Insert

   KiAA0010 (UBE3C )
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 17, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pGL2B -938/+64

   Plasmid

   #24943

   Depositor

   Stephen Smale

   Article

   Brightbill et al J Immunol. 2000 Feb 15. 164(4):1940-51.

   Insert

   IL-10 promoter (Il10 Mouse)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mutation

   Promoter

   Available since

   Aug. 18, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pCAP-BAC

   Plasmid

   #120229

   Purpose

   TAR cloning in yeast and low-copy maintenance of large constructs in E. coli

   Depositor

   Bradley Moore

   Article

   Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. hTRAF2pLPCX-HA-Flag/P874

    Plasmid

    #20229

    Depositor

    Martin Dorf

    Article

    Li et al Mol Cell. 2009 Jan 16. 33(1):30-42.

    Insert

    TNF receptor-associated factor 2 (TRAF2 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    unmutated NM_021138.3. L replaces M at beginning…

    Promoter

    Available since

    March 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLA230

    Plasmid

    #11724

    Depositor

    Lawrence Loeb

    Article

    Shinkai et al J Biol Chem. 2001 Dec 14. 276(50):46759-

    Insert

    b-lactamase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GAA for Glu26 changed to TAA (premature stop codo…

    Promoter

    Available since

    Aug. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. FLAG-SENP2m

    Plasmid

    #18713

    Depositor

    Edward Yeh

    Article

    Kang et al Mol Cell. 2010 Apr 23. 38(2):191-201.

    Insert

    SENP2 (SENP2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    R577L, K578M

    Promoter

    Available since

    June 20, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pN3-3xFlag-Control

    Plasmid

    #107717

    Purpose

    Control expression plasmid for 3xFLAG-tagged pN3-contructs

    Depositor

    Guntram Suske

    Article

    Stielow et al PLoS Genet. 2018 Jan 30;14(1):e1007193.

    Type

    Empty backbone

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pHpgRNA13

    Plasmid

    #160127

    Purpose

    To express a sgRNA with constant targeting ADE2 by RNA pol II promoter PTEF1

    Depositor

    Yongjin Zhou

    Article

    Gao et al iScience. 2021 Feb 9;24(3):102168. doi:

    Insert

    PTEF1-HH-gRNA-ADE2- HDV-TAMO
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. p70a-T7RNAP

    Plasmid

    #105683

    Purpose

    Expresses T7 polymerase in cell-free TXTL.

    Depositor

    Chase Beisel

    Article

    Maxwell et al Methods. 2018 Jul 1;143:48-57. doi: 10.1

    Insert

    T7 polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    OR2-OR1-Pr (bacteriophage Lambda with one mutatio…

    Available since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX-RIP

    Plasmid

    #11972

    Depositor

    Jon Ashwell

    Article

    Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

    Insert

    RIP (Ripk1 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 13, 2006

    Availability

    Academic Institutions and Nonprofits only

17. mFGF8b

    Plasmid

    #22090

    Depositor

    Gail Martin

    Article

    Crossley et al Development. 1995 Feb . 121(2):439-51.

    Insert

    FGF8 (Fgf8 Mouse)
    

    Use

    Bacterial

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pmCherry-FKBP12-C1

    Plasmid

    #67900

    Purpose

    Expresses a fusion of mCherry with FKBP12 in mammalian cells

    Depositor

    Dorus Gadella

    Article

    van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

    Insert

    mCherry-FKBP12
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSMF2

    Plasmid

    #112819

    Purpose

    Expresses wild-type Rpb1 in yeast cells

    Depositor

    Stephen Fuchs

    Article

    Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

    Insert

    RPB1 (RPO21 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Native promoter

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. SOX2se-CPG-TV

    Plasmid

    #70155

    Purpose

    a targeting vector to target the endogenous Sox2 SE CPG island with Snrp-Tomato-polyA

    Depositor

    Rudolf Jaenisch

    Article

    Stelzer et al Cell. 2015 Sep 24;163(1):218-29. doi: 10

    Insert

    5'hSOX2se-Snrp-Tomato-polyA-Pgk-PURO-3'hSOX2se
    

    Use

    Mouse Targeting and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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