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Showing: 2681 - 2700 of 4038 results

  1. pETDuet-msGC-NT21

    Plasmid
    #78102
    Purpose
    expresses the N-terminal fragment of manduca sexta soluble guanylyl cyclase subunits alpha and beta
    Depositor
    William Montfort
    Article
    Fritz et al Biochemistry. 2013 Mar 5;52(9):1568-82.
    Inserts
    fragment of alfa subunit of sGC
    fragment of beta subunit of sGC
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    fragment 1-380 of the beta subunit and fragment 2…
    Promoter
    T7
    Available since
    June 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    78102-plasmid-map-sequence-id-151428

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  2. pETDuet-msGC-NT13

    Plasmid
    #75087
    Purpose
    expresses the N-terminal fragment of manduca sexta soluble guanylyl cyclase subunits alpha and beta
    Depositor
    William Montfort
    Article
    Fritz et al Biochemistry. 2013 Mar 5;52(9):1568-82.
    Inserts
    fragment of alfa subunit of sGC
    fragment of beta subunit of sGC
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    fragment 1-380 of the beta subunit and fragment 4…
    Promoter
    Available since
    May 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    75087-plasmid-map-sequence-id-148282

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  3. AcrIIC1X* (AcrIIC1X-mCherry chimera)

    Plasmid
    #128114
    Purpose
    Expresses AcrIIC1(N3F/D15Q/A48I) fused to mCherry; mediates potent inhibition of both, N. meningitidis as well as S. aureus Cas9
    Depositor
    Dominik Niopek
    Article
    Mathony et al Nat Chem Biol. 2020 Apr 13. pii: 10.1038
    Insert
    AcrX* (AcrIIC1 N3F/D15Q/A48I fused to mCherry)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    128114-plasmid-map-sequence-id-249192

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  19. pLenti_EF1α-NES-Kinprola_on-mEGFP_bGH-PA term_EF1α_FLAG-SV40NLS-Cas9-NLS-T2A-BSD-WPRE-SV40

    Plasmid
    #233373
    Purpose
    EF1α driven co-expression of the consitutively active kinase activity recorder positive control Kinprola_on fused to mEGFP and Cas9 expression through lentivirus transduction
    Depositor
    Kai Johnsson
    Article
    Sun et al bioRxiv 2024.09.11.611894
    Inserts
    NES-Kinprola_on-mEGFP
    FLAG-SV40NLS-Cas9-NLS-T2A-BSD
    Use
    Lentiviral
    Tags
    FLAG, NES, and mEGFP
    Expression
    Mutation
    Promoter
    EF1α
    Available since
    March 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    233373-plasmid-map-sequence-id-466778

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  20. pLenti_EF1α-NES-Kinprola_PKA_T/A-mEGFP_bGH-PA term_EF1α_FLAG-SV40NLS-Cas9-NLS-T2A-BSD-WPRE-SV40

    Plasmid
    #233372
    Purpose
    EF1α driven co-expression of the inactive PKA activity recorder negative control with T/A mutation Kinprola_PKA_T/A fused to mEGFP and Cas9 expression through lentivirus transduction
    Depositor
    Kai Johnsson
    Article
    Sun et al bioRxiv 2024.09.11.611894
    Inserts
    NES-Kinprola_PKA_T/A-mEGFP
    FLAG-SV40NLS-Cas9-NLS-T2A-BSD
    Use
    Lentiviral
    Tags
    FLAG, NES, and mEGFP
    Expression
    Mutation
    Promoter
    EF1α
    Available since
    March 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    233372-plasmid-map-sequence-id-466777

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Showing: 2681 - 2700 of 4038 results