We narrowed to 4,099 results for: LYC

1. PVS10

   Plasmid

   #104398

   Purpose

   polycistronic vector for expression of E. coli core RNAP

   Depositor

   Irina Artsimovitch

   Article

   Svetlov et al Methods Mol Biol. 2015;1276:13-29. doi:

   Insert

   rpoA-rpoB-rpoC-6xHis and rpoZ (rpoA, rpoB, rpoC, rpoZ E. coli)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT22-GluCNZ-6His

   Plasmid

   #213504

   Purpose

   Designed for cytoplasmic E.coli overexpression of glucanase (β-1,3-glycosidase) for gentle yeast spheroblasting that unlike Zymolyase prevents proteolysis of intracellular components.

   Depositor

   John Denu

   Article

   Kuznetsov et al J Biol Chem. 2018 Aug 3;293(31):12271-12

   Insert

   Glucanase (glucan endo-1,3-beta-glucosidase)
   

   Use

   Tags

   6His tag

   Expression

   Bacterial

   Mutation

   1-36 Signal Sequence Deletion

   Promoter

   T7

   Available Since

   March 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19/ER-mChe

   Plasmid

   #183163

   Purpose

   Construct for transient expression of endoplasmic reticulum-targeted mCherry in plants.

   Depositor

   Yao-Guang Liu, Qinlong Zhu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Insert

   ER-mCherry
   

   Use

   Tags

   25 aa ER signal of Glycine soja kunitz-type tryps…

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. L2383-FRB-GPA-NLuc

   Plasmid

   #222902

   Purpose

   Positive control receptor chain for Nanoluciferase signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase.

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FRB-GPA-20GS-NanoLuc
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR-HLA-A2_GFP

   Plasmid

   #85161

   Purpose

   HLA.A2_GFP fusion with 2 glycines in between

   Depositor

   Sébastien Walchli, Tone Fredsvik Gregers

   Article

   Walchli et al Eur J Immunol. 2014 Mar;44(3):774-84. do

   Insert

   HLA.A2 (HLA-A Human)
   

   Use

   Gateway entry vector

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. YFP-Cdc42(V12)

   Plasmid

   #11399

   Depositor

   Joel Swanson

   Article

   Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

   Insert

   cell division cycle 42 (CDC42 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Obtained with Glycine 12 changed to Valine.

   Promoter

   Available Since

   Feb. 21, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKC52

   Plasmid

   #226780

   Purpose

   Expresses Drosophila UNC-104(R348G) in insect cells

   Depositor

   Kyoko Chiba

   Article

   Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

   Insert

   Drosophila unc-104 (R348G) (unc-104 Fly)
   

   Use

   Tags

   sfGFP and 2xStrepII

   Expression

   Insect

   Mutation

   changed Arginine 348 to Glycine

   Promoter

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET21b-RBD-JA1

   Plasmid

   #167126

   Purpose

   Expresses SARS-CoV-2 RBD in bacterial system

   Depositor

   David Margulies

   Article

   Ahmad et al bioRxiv. 2021 Jan 27. doi: 10.1101/2021.

   Insert

   SARS-CoV-2 Receptor Binding domain (S SARS-CoV-2)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   residues (333 to 529 aa)

   Promoter

   T7

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-SARS-CoV-2-S (D614G)

   Plasmid

   #215838

   Purpose

   Expresses SARS-CoV-2 Spike protein in mammalian cells

   Depositor

   Thomas Gallagher

   Article

   Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

   Insert

   SARS-CoV-2 Spike (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. p1A

    Plasmid

    #162692

    Purpose

    Expresses H. neapolitanus CbbLS and S. elongatus Prk. Rescues CCMB1 in M9 glycerol at 10% CO2

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    H. neapolitanus CbbLS and S. elongatus Prk
    

    Use

    Tags

    N terminal 6x His tag on PRK

    Expression

    Bacterial

    Mutation

    Promoter

    PLtet0-1 promoter

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOME0182

    Plasmid

    #178403

    Purpose

    Expresses KSHV K8.1 glycoprotein hyp tagged

    Depositor

    Dirk Dittmer

    Article

    Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

    Insert

    KSHV K8.1
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDGE476

    Plasmid

    #153267

    Purpose

    M2E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA-c/o NL4-3 Env delta CT

    Plasmid

    #139285

    Purpose

    HIV-1 NL4-3 glycoprotein lacking cytoplasmic tail

    Depositor

    Jeremy Luban

    Article

    Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

    Insert

    codon optimized HIV-1 Env delta cytoplasmic tail
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deletion of cytoplasmic tail after residue 710

    Promoter

    CMV IE

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-CMV-NGLY1-Puro

    Plasmid

    #181916

    Purpose

    Lentiviral plasmid that directs the expression of NGLY1 in mammalian cells.

    Depositor

    Scott Dixon

    Article

    Forcina et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

    Insert

    N-glycanase 1 (NGLY1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMK-Cas9-gate

    Plasmid

    #113743

    Purpose

    Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving aminoglycoside phosphotransferase for G418 selection in plants.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    Cas9
    

    Use

    CRISPR; Gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    Z. Mays ubiquitin promoter, 35S promoter

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRS1422

    Plasmid

    #12548

    Depositor

    Robert Sobol

    Article

    Trivedi et al Cancer Res. 2005 Jul 15. 65(14):6394-400

    Insert

    human Methylpurine DNA glycosylase (MPG Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQE-30-RexYFP

    Plasmid

    #141070

    Purpose

    Bacterial expression of genetically encoded fluorescent indicator for measuring NAD+/NADH ratio

    Depositor

    Vsevolod Belousov

    Article

    Bilan et al Biochim Biophys Acta. 2013 Nov 25. pii:

    Insert

    RexYFP
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    changed Leucine 169 to Proline, changed Tyrosine …

    Promoter

    T5

    Available Since

    March 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. T2A-mCherry (MTK 4a) (pAN3844)

    Plasmid

    #194223

    Purpose

    Mammalian Toolkit part 4a encoding a T2A polycistronic element followed by mCherry

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Insert

    T2A-mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    None

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDGE657

    Plasmid

    #153255

    Purpose

    M2E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GFP-Talin-L432G

    Plasmid

    #26725

    Depositor

    Anna Huttenlocher

    Article

    Franco et al Nat Cell Biol. 2004 Oct . 6(10):977-83.

    Insert

    Talin (Tln1 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Changed Leucine 432 to Glycine to block ability o…

    Promoter

    Available Since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only