Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 18,057 results for: puro

Clear keywords
Showing: 4051 - 4080 of 18057 results

  1. LIR-wtloxP-TRE-CAG-Frt-red-puro-3xPA-Frt-GFP(mVenus)-KrasG12D-cMyc-SV40LT-IRES-KRABoff-PA-TRE-loxP257-RIR

    Plasmid
    #67277
    Purpose
    inducbile color change and cancer regulation: FlpO recombinase dependent expression of KrasG12D cMyc and SV40 large T antigen with doxycycline inducible downregulation through tetKRAB system.
    Depositor
    Jannik Elverløv-Jakobsen
    Article
    Callesen et al Mol Oncol. 2017 Nov;11(11):1616-1629. do
    Inserts
    first casette contain a red fluorescent protein (katushka) linked to puromycin resitance gene through an E2A site
    second cassette contains mVENUS-kras-cmyc-SV40LT-KRABoff
    Tags
    mTQ2 blue fluorescent and red flourescent gene ka…
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Aug. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    67277-plasmid-map-sequence-id-121189

    Enlarge

  2. pCDH-EF1-copGFP

    Plasmid
    #73030
    Purpose
    Express copGFP
    Depositor
    Kazuhiro Oka
    Insert
    copGFP
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1
    Available Since
    Feb. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    73030-plasmid-map-sequence-id-427120

    Enlarge

  3. pCDH-CMV-Nluc

    Plasmid
    #73038
    Purpose
    Express Nluc reporter
    Depositor
    Kazuhiro Oka
    Insert
    Nluc
    Use
    Lentiviral and Luciferase
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    73038-plasmid-map-sequence-id-136036

    Enlarge

  4. pCDH-EF1S-Nluc

    Plasmid
    #73033
    Purpose
    Express Nluc
    Depositor
    Kazuhiro Oka
    Insert
    Nluc
    Use
    Lentiviral and Luciferase
    Expression
    Mammalian
    Promoter
    Truncated EF1
    Available Since
    Jan. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    73033-plasmid-map-sequence-id-133974

    Enlarge

  5. pCDH-EF1S-copGFP

    Plasmid
    #73034
    Purpose
    Express copGFP reporter
    Depositor
    Kazuhiro Oka
    Insert
    copGFP
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    Truncated EF1
    Available Since
    Jan. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    73034-plasmid-map-sequence-id-133977

    Enlarge

  6. pCDH-EF1-Nluc

    Plasmid
    #73024
    Purpose
    Express Nluc reporter under EF1 promoter
    Depositor
    Kazuhiro Oka
    Insert
    Nluc
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1
    Available Since
    Jan. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    73024-plasmid-map-sequence-id-133955

    Enlarge

  7. pGMC00009 (aka pRC0494)

    Plasmid
    #195294
    Purpose
    Lenti-CRISPR-V2-GFP-2A-Puro - vector with Cas9/sgRNA backbone/Puro/GFP
    Depositor
    Raj Chari
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Expression
    Mammalian
    Available Since
    Feb. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    195294-plasmid-map-sequence-id-385588

    Enlarge

  8. pGMC00010 (aka pRC0495)

    Plasmid
    #195295
    Purpose
    Lenti-CRISPR-V2-RFP-2A-Puro - vector with Cas9/sgRNA backbone/Puro/RFP
    Depositor
    Raj Chari
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Expression
    Mammalian
    Available Since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    195295-plasmid-map-sequence-id-387224

    Enlarge

  9. pIRES Centrin1 GFP Pur

    Plasmid
    #64341
    Purpose
    Bicistronic expression of Centrin-GFP fusion and Puro resistance gene
    Depositor
    Matthieu Piel
    Insert
    Centrin1 (CETN1 Human)
    Tags
    GFP and IRES-Puro
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 19, 2015
    Availability
    Academic Institutions and Nonprofits only

  10. XLone-eGFP NLS (no drug resistant gene)

    Plasmid
    #140032
    Purpose
    Tunable and temporal expression control of eGFP (or gene of interest) with constitutive nuclear eGFP
    Depositor
    Xiaoping Bao
    Insert
    Enhanced Green Fluorescent Protein
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    TRE3G
    Available Since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    140032-plasmid-map-sequence-id-275878

    Enlarge

  11. lentiCRISPRv2_PROX1_1+3

    Plasmid
    #121176
    Purpose
    Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.
    Depositor
    Timo Otonkoski
    Insert
    Cas9-P2A-puro and U6 promoters driven expression of gRNAs
    Use
    CRISPR and Lentiviral
    Tags
    P2A-puro
    Expression
    Mammalian
    Available Since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    121176-plasmid-map-sequence-id-235001

    Enlarge

  12. lentiCRISPRv2_PROX1_1+4

    Plasmid
    #121177
    Purpose
    Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.
    Depositor
    Timo Otonkoski
    Insert
    Cas9-P2A-puro and U6 promoters driven expression of gRNAs
    Use
    CRISPR and Lentiviral
    Tags
    P2A-puro
    Expression
    Mammalian
    Available Since
    March 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    121177-plasmid-map-sequence-id-237896

    Enlarge

  13. px330_SMAD exon 2 gRNA

    Plasmid
    #68350
    Purpose
    px330 with gRNA towards SMAD exon 2. Cas9 is expressed from a CAG promoter.
    Depositor
    Jannik Elverløv-Jakobsen
    Insert
    SMAD exon 2 gRNA
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68350-plasmid-map-sequence-id-119622

    Enlarge

  14. px330_P53 exon 8 gRNA

    Plasmid
    #68349
    Purpose
    px330 with gRNA towards P53 exon 8. Cas9 is expressed from a CAG promoter.
    Depositor
    Jannik Elverløv-Jakobsen
    Insert
    P53 exon 8 gRNA
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68349-plasmid-map-sequence-id-119621

    Enlarge

  15. px330_P53 exon 7 gRNA

    Plasmid
    #68351
    Purpose
    px330 with gRNA towards P53 exon 7. Cas9 is expressed from a CAG promoter.
    Depositor
    Jannik Elverløv-Jakobsen
    Insert
    P53 exon 7 gRNA
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68351-plasmid-map-sequence-id-119623

    Enlarge

  16. px330_PTEN exon 5 gRNA

    Plasmid
    #68348
    Purpose
    px330 with gRNA towards PTEN exon 5. Cas9 is expressed from a CAG promoter.
    Depositor
    Jannik Elverløv-Jakobsen
    Insert
    PTEN exon 5 gRNA
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68348-plasmid-map-sequence-id-119620

    Enlarge

  17. pSSA-RPG

    Plasmid
    #85932
    Purpose
    Constitutively express DsRed and conditionally express Puro and EGFP. Used to test transfection efficiency and enrich genetically modified cells
    Depositor
    Zhiying Zhang
    Article
    Ren et al Cell Mol Life Sci. 2015 Jul;72(14):2763-
    Insert
    pPB-CMV-DsRed-polyA-CAG-SSA. Puro-T2A-EGFP
    Use
    Dual-reporter surrogate
    Expression
    Mammalian
    Available Since
    Feb. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85932-plasmid-map-sequence-id-172472

    Enlarge

  18. pNHEJ-RPG

    Plasmid
    #85931
    Purpose
    Constitutively express DsRed and conditionally express Puro and EGFP. Used to test transfection efficiency and enrich genetically modified cells
    Depositor
    Zhiying Zhang
    Article
    Ren et al Cell Mol Life Sci. 2015 Jul;72(14):2763-
    Insert
    pPB-CMV-DsRed-polyA-CAG-NHEJ. Puro-T2A-EGFP
    Use
    Dual-reporter surrogate
    Expression
    Mammalian
    Available Since
    Feb. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85931-plasmid-map-sequence-id-172473

    Enlarge

  19. XLone-BSD SPI1 P2A mCherry

    Plasmid
    #140031
    Purpose
    Tunable and temporal expression control of SPI1 and mCherry
    Depositor
    Xiaoping Bao
    Insert
    SPI1 (SPI1 Human)
    Use
    Synthetic Biology
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    TRE3G
    Available Since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    140031-plasmid-map-sequence-id-275874

    Enlarge

  21. px330_SMAD exon 9 gRNA

    Plasmid
    #68352
    Purpose
    px330 with gRNA towards SMAD exon 9. Cas9 is expressed from a CAG promoter.
    Depositor
    Jannik Elverløv-Jakobsen
    Insert
    SMAD exon 9 gRNA
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68352-plasmid-map-sequence-id-119624

    Enlarge

  22. XLone-BSD NFIL3 P2A eGFP NLS

    Plasmid
    #140030
    Purpose
    Tunable and temporal expression control of NFIL3 and nuclear eGFP
    Depositor
    Xiaoping Bao
    Insert
    NFIL3 (NFIL3 Human, Synthetic)
    Use
    Synthetic Biology
    Tags
    eGFP
    Expression
    Mammalian
    Promoter
    TRE3G
    Available Since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    140030-plasmid-map-sequence-id-275866

    Enlarge

  23. HF-PX459 (V2)

    Plasmid
    #118632
    Purpose
    Plasmid encoding SpCas9-HF1, a single guide RNA and puromycin resistance
    Depositor
    Mike McGrew
    Article
    Idoko-Akoh et al Sci Rep. 2018 Oct 11;8(1):15126. doi: 10
    Insert
    hSpCas9-HF1-2A-Puro V2.0
    Use
    CRISPR
    Tags
    2A-Puro and 3XFLAG
    Expression
    Mammalian
    Mutation
    Asparagine 497 to Alanine, Arginine 661 to Alanin…
    Promoter
    Cbh
    Available Since
    Dec. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    118632-plasmid-map-sequence-id-230217

    Enlarge

  25. pBK1837

    Plasmid
    #239840
    Purpose
    7. BK1837- pLV-CMV-SNCAintron1-AlphaSyn-d2EGFP-P2A-NLuc-SV40-Puro-WPRE
    Depositor
    Boris Kantor
    Article
    Kantor et al Mol Ther. 2018 Nov 7;26(11):2638-2649. d
    Inserts
    pLV-CMV-SNCAintron1-AlphaSyn-d2EGFP-P2A-NLuc-SV40-Puro-WPRE (pCL35)
    pLV-CMV-SNCAintron1-AlphaSyn-d2EGFP-P2A-NLuc-SV40-Puro-WPRE (pCL35)
    Use
    Lentiviral
    Promoter
    CMV
    Available Since
    Nov. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    239840-plasmid-map-sequence-id-477923

    Enlarge

  27. PX459v3

    Plasmid
    #178799
    Purpose
    SpCas9 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Cbh (Cas9-2A Puro) and U6 (gRNA)
    Available Since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    178799-plasmid-map-sequence-id-354842

    Enlarge

  28. CROPseq-3P5P

    Plasmid
    #219680
    Purpose
    CROPseq vector enabling gRNA detection via mRNA-based capture (CROPseq), 3'-based direct capture using 10x capture sequence 1 or 5'-based enrichment using a custom reverse transcription primer
    Depositor
    Christoph Bock
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    EF1a-Puro-P2A-mCherry2-WPRE-hU6-gRNA
    Expression
    Mammalian
    Available Since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    219680-plasmid-map-sequence-id-462884

    Enlarge

  29. pdx1-mTQ2-P2A-FlpO-PA

    Plasmid
    #67279
    Purpose
    Pancreas specific expression of the FlpO recombinase controlled by the pdx1 promoter . FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon context
    Depositor
    Jannik Elverløv-Jakobsen
    Inserts
    mTQ2=mturquoise2 blue fluorescent gene
    FlpO recombinase
    Tags
    linked to FlpO through an P2A ribosomal skipping …
    Expression
    Mammalian
    Promoter
    pdx
    Available Since
    July 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    67279-plasmid-map-sequence-id-115888

    Enlarge

Showing: 4051 - 4080 of 18057 results