We narrowed to 50,997 results for: ANG

1. pYPQ292 (AaCas12b)-D570A-TV-MS2-VPR

   Plasmid

   #136382

   Purpose

   CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-VPR fusion protein

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   AaCas12b (D570A)-TV-T2A-MCP-VPR
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   D570A

   Available Since

   Feb. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-MtAcKRS (human-opti)

   Plasmid

   #164195

   Purpose

   To express a Methanosarcina thermophila derived AcKRS in mammalian cells for genetic code expansion

   Depositor

   Tao Liu

   Article

   Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2

   Insert

   MtAcKRS-478(human-opti)
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Mutation

   D76G; L325M; L329I; Y330F; L333A; C372F

   Promoter

   CMV

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAC84-pCR8-dCas9

   Plasmid

   #48218

   Purpose

   dCas9 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9(D10A;H840A)
   

   Use

   CRISPR; Gateway donor

   Tags

   HA Tag

   Mutation

   D10A;H840A

   Available Since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBT3024

   Plasmid

   #122542

   Purpose

   Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a mitochondrial target peptide (mt) under the intestinal specific promoter nhx-2

   Depositor

   Gary Silverman

   Article

   Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

   Insert

   Pnhx-2_mt::CemOrange2
   

   Tags

   MLSLRQSIRFFKPATRTLCSSRTLL

   Expression

   Bacterial

   Promoter

   Pnhx-2

   Available Since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. SARS_COV2_ORF3b_HA_BASU

   Plasmid

   #153992

   Purpose

   Express Orf3b gene along with HA and BASU

   Depositor

   Paul Khavari

   Article

   Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

   Insert

   orf3b
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. Ad-EFNA1

   Plasmid

   #102724

   Purpose

   Expression of EphrinA1

   Depositor

   Jin Chen

   Article

   Brantley-Sieders et al Cancer Res. 2006 Nov 1;66(21):10315-24.

   Insert

   EFNA1 (EFNA1 Human)
   

   Use

   Adenoviral

   Available Since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAC5-dual-dCas9VP48-sgTetO

   Plasmid

   #48237

   Purpose

   Dual expression construct expressing both dCas9VP48 and sgTetO from separate promoters

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9VP48 and sgTetO
   

   Use

   CRISPR

   Tags

   HA-tag and VP48

   Expression

   Mammalian

   Mutation

   D10A H840A (catalytically inactive)

   Available Since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAS4.1w-tet-on-NORAD

   Plasmid

   #125010

   Purpose

   express in mammalian cell

   Depositor

   Ruey-Hwa Chen

   Article

   Tan et al Oncogene. 2019 Jul;38(28):5612-5626. doi

   Insert

   Homo sapiens non-coding RNA activated by DNA damage (NORAD), long non-coding RNA (NORAD Human)
   

   Expression

   Mammalian

   Promoter

   TRE-Tight

   Available Since

   April 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. SARS_COV2_orf9c_HA_BASU

   Plasmid

   #153991

   Purpose

   Express Orf9c gene along with HA and BASU

   Depositor

   Paul Khavari

   Article

   Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

   Insert

   orf9c
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-MfPylRS (human-opti)

    Plasmid

    #164081

    Purpose

    To express a Methanosarcina flavescens derived PylRS in mammalian cells for genetic code expansion

    Depositor

    Tao Liu

    Article

    Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2

    Insert

    MfPylRS(human-opti)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDest27-mBIN1+13

    Plasmid

    #61087

    Purpose

    Express N-terminal GST tagged-mouse BIN1+13 in mammalian cells

    Depositor

    Robin Shaw

    Article

    Hong et al Nat Med. 2014 Jun;20(6):624-32. doi: 10.

    Insert

    mouse BIN1 with exon 13, excluding exon 7, 11, 14-17
    

    Tags

    GST

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pDest27-mBIN1

    Plasmid

    #61086

    Purpose

    Express N-terminal GST tagged-mouse BIN1 in mammalian cells

    Depositor

    Robin Shaw

    Article

    Hong et al Nat Med. 2014 Jun;20(6):624-32. doi: 10.

    Insert

    smallest constitutive BIN1excluding exon 7, 11, 13-17
    

    Tags

    GST

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

13. PhyB-mCherry-CDC10

    Plasmid

    #51568

    Purpose

    Constitutively express bud neck localized PhyB in Saccharomyces cerevisiae

    Depositor

    Chao Tang

    Article

    Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

    Insert

    PhyB (PHYB Mustard Weed)
    

    Tags

    CDC10 and mCherry

    Expression

    Yeast

    Mutation

    1-908aa only

    Promoter

    ADH1pr

    Available Since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. PhyB-mCherry-Spc72

    Plasmid

    #51582

    Purpose

    Constitutively express spindle pole body localized PhyB in Saccharomyces cerevisiae

    Depositor

    Chao Tang

    Article

    Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

    Insert

    PhyB (PHYB Mustard Weed)
    

    Tags

    Spc72 and mCherry

    Expression

    Yeast

    Mutation

    1-908aa only

    Promoter

    ADH1pr

    Available Since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PAX6 sgRNA7

    Plasmid

    #68466

    Purpose

    targeting PAX6 gene

    Depositor

    Su-Chun Zhang

    Article

    Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

    Insert

    sgRNA targeting PAX6 (PAX6 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDest27-mBIN1+17

    Plasmid

    #61096

    Purpose

    Express N-terminal GST tagged-mouse BIN1+17 in mammalian cells

    Depositor

    Robin Shaw

    Article

    Hong et al Nat Med. 2014 Jun;20(6):624-32. doi: 10.

    Insert

    mouse BIN1 with exon 17, excluding exon 7, 11, 13-16
    

    Tags

    GST

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pAC92-pmax-dCas9VP96

    Plasmid

    #48224

    Purpose

    dCas9VP96 on pmax expression vector

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A) fusion with VP96 activation domain
    

    Use

    CRISPR

    Tags

    HA Tag and VP96

    Expression

    Mammalian

    Mutation

    D10A;H840A

    Promoter

    CAGGS

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBT3019

    Plasmid

    #122541

    Purpose

    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a peroxisome target peptide (SKL) under the intestinal specific promoter nhx-2

    Depositor

    Gary Silverman

    Article

    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

    Insert

    Pnhx-2_CemOrange2::SKL
    

    Tags

    SKL

    Expression

    Bacterial

    Promoter

    Pnhx-2

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pYPQ292 (AaCas12b)-D570A-TV-MS2-TV

    Plasmid

    #136381

    Purpose

    CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-TV fusion protein

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AaCas12b (D570A)-TV-T2A-MCP-TV
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D570A

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-Nxph4-4Q-HA

    Plasmid

    #128104

    Purpose

    Express HA tagged Nxph4-4Q

    Depositor

    Huda Zoghbi

    Article

    Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

    Insert

    Nxph4 (Nxph4 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pEGFP-C2_3X Flag

    Plasmid

    #194161

    Purpose

    Expresses eGFP fluorescent protein with a 3X flag tag

    Depositor

    Stefan Stamm

    Article

    Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

    Insert

    GFP with 3X Flag
    

    Tags

    3x Flag

    Expression

    Mammalian

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. PAX6 sgRNA2

    Plasmid

    #68465

    Purpose

    targeting PAX6 gene

    Depositor

    Su-Chun Zhang

    Article

    Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

    Insert

    sgRNA targeting PAX6 (PAX6 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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23. pminiT-Halo-MED1 donor

    Plasmid

    #197868

    Purpose

    Donor plasmid used for building Halo-MED1 knock-in mESCs

    Depositor

    James Zhe Liu

    Article

    Dong et al bioRxiv 2022.10.26.513917

    Insert

    CRSP210 (Med1 Mouse)
    

    Use

    Mouse Targeting

    Tags

    Halotag

    Promoter

    NA

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV-MtPylRS (human-opti)

    Plasmid

    #164080

    Purpose

    To express a Methanosarcina thermophila derived PylRS in mammalian cells for genetic code expansion

    Depositor

    Tao Liu

    Article

    Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2

    Insert

    MtPylRS-478(human-opti)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCMV-MfBulKRS (human-opti)

    Plasmid

    #164196

    Purpose

    To express a Methanosarcina flavescens derived BulKRS in mammalian cells for genetic code expansion

    Depositor

    Tao Liu

    Article

    Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2

    Insert

    Mf BulKRS(human-opti)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Mutation

    Y269A;Y347F

    Promoter

    CMV

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PhyB-mCherry-MYO1

    Plasmid

    #51570

    Purpose

    Constitutively express myosin ring localized PhyB in Saccharomyces cerevisiae

    Depositor

    Chao Tang

    Article

    Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

    Insert

    PhyB (PHYB Mustard Weed)
    

    Tags

    MYO1 and mCherry

    Expression

    Yeast

    Mutation

    1-908aa only

    Promoter

    ADH1pr

    Available Since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. IBMc326

    Plasmid

    #161949

    Purpose

    Contains Type IIS restriction site free elements for expression vectors in IBM. Expression vector to generate ORF insertion library in IBM on ECF20

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-5)-BsaI-BsaI-ECF20(187-193)-Ter
    

    Expression

    Bacterial

    Promoter

    PBAD

    Available Since

    March 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCLXSN(GFP)-HA-Tbkbp1

    Plasmid

    #125177

    Purpose

    Retroviral expression of mouse Tbkbp1

    Depositor

    Shao-Cong Sun

    Article

    Zhu et al Nat Commun. 2018 Jul 18;9(1):2812. doi:

    Insert

    Tbkbp1 (Tbkbp1 Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAC1-pCR8-dCas9VP48

    Plasmid

    #48214

    Purpose

    dCas9VP48 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A) fusion with VP48 activation domain
    

    Use

    CRISPR; Gateway donor

    Tags

    HA Tag and VP48

    Mutation

    D10A;H840A

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. kank3_pJet1.2

    Plasmid

    #121981

    Purpose

    for generating kank3 riboprobes for WISH in zebrafish

    Depositor

    GuangJun Zhang

    Article

    Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

    Insert

    kank3 (kank3 Zebrafish)
    

    Use

    Cloning vector

    Promoter

    T7

    Available Since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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