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pRSETa-mKikGR-V69T

Plasmid

#98574

Purpose

photoconvertible fluorescent protein mKikGR-V69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coli

Depositor

Ulrike Endesfelder

Article

Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

Insert

mKikGR-V69T

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2018

Availability

Academic Institutions and Nonprofits only

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p3HD1RLuc2

Plasmid

#236276

Purpose

Mammalian expression of human dopamine D1 receptor; N-terminal HA tag, C-terminal Rluc8

Depositor

Vsevolod Gurevich

Article

Gimenez et al J Biol Chem. 2012 Aug 24;287(35):29495-5

Insert

dopamine D1 receptor (DRD1 Human)

Tags

3xHA and Rluc8

Expression

Mammalian

Promoter

CMV

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pHH0103_TP53BP2-1/1

Plasmid

#91503

Purpose

Protein expression and purification of human SH3 domain construct TP53BP2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

TP53BP2-1/1 (TP53BP2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-shRNA_Tet3

Plasmid

#85740

Purpose

shRNA against mouse Tet3

Depositor

Hongjun Song

Article

Yu et al Nat Neurosci. 2015 Jun;18(6):836-43. doi

Insert

Tet3 (Tet3 Mouse)

Use

AAV

Tags

EYFP

Expression

Mammalian

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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ETV7_pet28a

Plasmid

#131656

Purpose

Bacterial expression of ETS gene ETV7

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ETV7 (ETV7 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUD3 (OTU, aa 52-209)

Plasmid

#61412

Purpose

Expresses human OTUD3 (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD3 (OTUD3 Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-51 and aa 210-398.

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-DIO-CFL-SN

Plasmid

#181740

Purpose

AAV vector for expressing CFL-SN

Depositor

Yasunori Hayashi

Article

Goto et al Science. 2021 Nov 12;374(6569):857-863.

Insert

CFL-SN

Use

AAV

Expression

Mammalian

Promoter

EF1alpha

Available Since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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Sc++

Plasmid

#155011

Purpose

Expresses the codon-optimized, engineered Sc++ Cas9 nuclease in mammalian cells

Depositor

Joseph Jacobson

Article

Chatterjee et al Nat Biotechnol. 2020 May 11. pii: 10.103

Insert

Sc++

Expression

Mammalian

Mutation

T1227K, Loop Substitution from S. anginosus

Promoter

EF1ɑ

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUD2 (full-length, aa 1-348)

Plasmid

#61409

Purpose

Expresses human OTUD2 (full-length) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD2 (YOD1 Human)

Tags

His6-GST-3C

Expression

Bacterial

Promoter

T7

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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MSP469

Plasmid

#65771

Purpose

Human expression vector for SpCas9 VQR variant: CMV-T7-humanSpCas9(D1135V/R1335Q/T1337R)-NLS-3xFLAG (VQR variant)

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/R1335Q/T1337R)-NLS-3XFlag

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Mammalian

Mutation

D1135V, R1335Q, and T1337R mutations in Cas9

Promoter

CMV & T7

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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UAST-FRT-GFP-FRT-uMCas12a+nls1x

Plasmid

#230908

Purpose

Double inducible expression of Cas12a+ with Gal4 and Flp.

Depositor

Michael Boutros

Article

Port et al Nat Commun. 2026 Jan 15;17(1):877. doi:

Insert

Cas12a+

Use

CRISPR

Expression

Insect

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pmTurqoise KIF1A shRNA

Plasmid

#102849

Purpose

Generates shRNA specific to Rat KIF1A along with cell fill pmTurqoise

Depositor

Erik Dent

Article

McVicker et al Nat Commun. 2016 Sep 23;7:12741. doi: 10

Insert

KIF1A-shRNA (KIF1A Rat)

Use

RNAi

Expression

Mammalian

Promoter

H1-RNA

Available Since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pJExpress401-KDAC8

Plasmid

#224208

Purpose

Expresses human KDAC8 (HDAC8) in E. coli

Depositor

Terry Watt

Article

Toro et al Protein Sci. 2015 Dec;24(12):2020-32. do

Insert

KDAC8 (HDAC8 Human)

Tags

TEV-cleavable His6

Expression

Bacterial

Promoter

T5

Available Since

Dec. 6, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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ELF1_pet28a

Plasmid

#131649

Purpose

Bacterial expression of ETS gene ELF1

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ELF1 (ELF1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 3, 2019

Availability

Academic Institutions and Nonprofits only

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LT3GEPIR-WT1shRNA

Plasmid

#220560

Purpose

To inducibly knockdown WT1 or EWSR1::WT1 expression

Depositor

Sean Lee

Article

Magrath et al Commun Biol. 2024 Apr 4;7(1):411. doi: 1

Insert

WT1 shRNA (WT1 Human)

Use

Lentiviral

Promoter

TRE3G (TetOP) promoter

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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FLAG-Skp2

Plasmid

#89142

Purpose

mammalian expression of human Skp2 with N-terminal FLAG tag

Depositor

Elizabeth Wilson

Article

Su et al Mol Cell Endocrinol. 2017 Jan 5;439:1-9.

Insert

Skp2 (SKP2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

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pDuet TDP-43 Q331K

Plasmid

#27465

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Use

Coexpression

Expression

Bacterial

Mutation

Glutamine 331 mutated to Lysine (Q331K)

Available Since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only

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CMV PGK1-3xHA

Plasmid

#220913

Purpose

Expresses PGK1-3xHA

Depositor

Timothy Ryan

Article

Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

Insert

PGK1-3xHA (PGK1 Human)

Use

Affinity Reagent/ Antibody

Tags

3xHA

Expression

Mammalian

Promoter

CMV

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pDonor-GDH

Plasmid

#140629

Purpose

Encodes a mini-transposon derived from V. cholerae Tn6677 and T7-GDH cargo gene.

Depositor

Sheng Yang

Article

Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

Insert

Mini-Tn6677, T7lac-GDH gene cassette

Use

CRISPR; Transposon

Expression

Bacterial

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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VEE-mScarlet3-IRES-EGFP-IRES-E3

Plasmid

#242408

Purpose

Self-amplifying RNA (saRNA) E3 construct; expresses the vaccinia virus E3 protein, which binds and sequesters dsRNA broadly inhibiting dsRNA-sensing pathways

Depositor

Ewan St. John Smith

Article

Lim et al bioRxiv 2024.09.24.614636

Inserts

mScarlet3
IRES-EGFP
IRES-E3

Use

Self-amplifying rna

Expression

Mammalian

Available Since

Sept. 4, 2025

Availability

Academic Institutions and Nonprofits only

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