We narrowed to 6,336 results for: WUS;

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pFSynW pHluorin-SYT9 IRES mRuby3

Plasmid

#195698

Purpose

Lentiviral plasmid encoding SYT9 with an N-terminal pHluorin tag followed by an internal ribosomal entry site followed by mRuby3 under the human synapsin promoter

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Rat)

Use

Lentiviral

Tags

pHluorin

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pFSynW Syt9 IRES mRuby3

Plasmid

#195700

Purpose

Lentiviral plasmid encoding full-length mouse SYT9 followed by an internal ribosomal entry site followed by mRuby under the human synapsin promoter

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Use

Lentiviral

Mutation

encodes only the first 68 amino acids of Tac1

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pFSynW SYT9 D197, 199N IRES GFP

Plasmid

#195701

Purpose

Lentiviral plasmid encoding SYT9 with D197, 199N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Use

Lentiviral

Mutation

full-length mouse SYT9 with D197, 199N mutations

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pFSynW SYT9 D330, 332N IRES GFP

Plasmid

#195702

Purpose

Lentiviral plasmid encoding SYT9 with D330, 332N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Use

Lentiviral

Mutation

full-length mouse SYT9 with D330, 332N mutations

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX SYT9 C2AB D197,199,330,332N

Plasmid

#195706

Purpose

Bacterial expression plasmid encoding GST-tagged SYT9 C2AB domain with D197, 199, 330, 332N mutations

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Tags

GST

Expression

Bacterial

Mutation

encodes amino acids 104-386 of mouse SYT9 with D1…

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-Best1-Txnip(C247S_LL351-352AA)

Plasmid

#206344

Purpose

AAV plasmid expressing mutant TXNIP in retinal pigment epithelium

Depositor

Connie Cepko

Article

Xue et al Elife. 2021 Apr 13;10. pii: 66240. doi:

Insert

Txnip (Txnip Mouse)

Use

AAV

Mutation

C247S, L351A, L352A

Promoter

human Best1

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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UAS-4x(tRNA::axed{sgRNA})

Plasmid

#187883

Purpose

Gal4/UAS sgRNA expression targeting axed

Depositor

Lukas Neukomm

Article

Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

Insert

4 sgRNAs targeting axed

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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UAS-4x(tRNA::hiw{sgRNA})

Plasmid

#187884

Purpose

Gal4/UAS sgRNA expression targeting hiw

Depositor

Lukas Neukomm

Article

Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

Insert

4 sgRNAs targeting hiw

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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UAS-4x(tRNA::dsarm{sgRNA})

Plasmid

#187885

Purpose

Gal4/UAS sgRNA expression targeting dsarm

Depositor

Lukas Neukomm

Article

Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

Insert

4 sgRNAs targeting dSarm

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pBABR mCherry-Bcd-LEXY

Plasmid

#182596

Purpose

Drosophila integration plasmid expressing mCherry-Bcd-LEXY fusion protein under the maternal tubulin promoter.

Depositor

Jared Toettcher

Article

Singh et al Cell Rep. 2022 Mar 22;38(12):110543. doi

Insert

Bcd (bcd Fly)

Tags

LEXY and mCherry

Expression

Insect

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV-Arkadia C946S H948L

Plasmid

#180373

Purpose

expressing mouse Arkadia (isoform 3) C946S H948L E3 ligase dead mutant proteins

Depositor

Dan Littman

Article

Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

Insert

Arkadia (Rnf111 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

C946S H948L

Promoter

LTR promoter

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pSIN-Skil-gRNA1

Plasmid

#180370

Purpose

targeting mouse Skil/SnoN gene

Depositor

Dan Littman

Article

Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

Insert

Skil targeting gRNA (Skil Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

human U6

Available Since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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pSIN-Ski-gRNA1

Plasmid

#180368

Purpose

targeting mouse Ski gene

Depositor

Dan Littman

Article

Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

Insert

Ski targeting gRNA (Ski Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

human U6

Available Since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_RalA(G23V,full)-P2A-Hygro_Barcode

Plasmid

#170249

Purpose

Barcoded lentiviral vector to express RalA(G23V, full) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

RALA (RALA Human)

Use

Lentiviral

Mutation

G23V

Promoter

EF1a

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 ZUC

Plasmid

#169964

Purpose

pDONR221 for Gateway shuttling. Contains the CARD8 FIIND and CARD (L162M-L537, no stop codon)

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Available Since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 FIIND L370G

Plasmid

#169970

Purpose

pDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (F370G)

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Mutation

L370G

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 FIIND L368G

Plasmid

#169969

Purpose

pDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (L368G)

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Mutation

L368G

Available Since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 PAM

Plasmid

#169975

Purpose

pDONR221 for Gateway shuttling. Contains CARD8 (no stop codon) with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Mutation

PAM

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 FIIND R394E

Plasmid

#169971

Purpose

pDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (R394E)

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Mutation

R394E

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 FIIND F405G

Plasmid

#169973

Purpose

pDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (F405G)

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Mutation

F405G

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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