We narrowed to 10,632 results for: t7

1. pET28c-F30-2xdBroccoli

   Plasmid

   #66843

   Purpose

   Expresses two dimeric Broccoli aptamers in the F30 scaffold in bacterial cells

   Depositor

   Samie Jaffrey

   Article

   Filonov et al Curr Protoc Chem Biol. 2016 Mar 16;8(1):

   Insert

   Two dimeric Broccoli aptamers in the F30 scaffold
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+)-OTI-TCRB

   Plasmid

   #131036

   Purpose

   Expresses OT-I TCRB

   Depositor

   Jeff Hammerbacher

   Article

   Aksoy et al bioRxiv

   Insert

   TCRB (Tcrb Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV/T7

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. 40 pCOLI_K_MBP

   Plasmid

   #160183

   Purpose

   N-term MBP 3C cleavable Kan resistant bacterial expression vector

   Depositor

   John Weir

   Article

   Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

   Type

   Empty backbone

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-24a-ELP[V-30]

   Plasmid

   #67014

   Purpose

   Expresses ELP[V-30] in E. coli

   Depositor

   Ashutosh Chilkoti

   Article

   Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

   Insert

   ELP[V-30]
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. Sumire-pRsetB

   Plasmid

   #193361

   Purpose

   GFP variant emit 414 nm fluorescence.

   Depositor

   Takeharu Nagai

   Article

   Sugiura et al Commun Biol. 2022 Nov 3;5(1):1172. doi:

   Insert

   Sumire
   

   Use

   E.coli expression

   Tags

   His X6 tag

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. 20 pCOLI_A_N-His_C_STREP

   Plasmid

   #160162

   Purpose

   N-term 6xHis 3C cleavable, C--term 2xStrepII Amp resistant bacterial expression vector

   Depositor

   John Weir

   Article

   Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

   Type

   Empty backbone

   Tags

   2xStrepII and 6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDF-RFP amber

   Plasmid

   #229524

   Purpose

   mCherry red fluorescent protein (RFP) reporter protein with an amber stop codon at position 13

   Depositor

   Thomas Huber

   Article

   Abdelkader et al Chem 102385

   Insert

   RFP amber
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28-Nsp14-TEV-Strep

   Plasmid

   #154953

   Purpose

   Expresses Nsp14 from SARS-CoV-2 with a C-terminal TEV-protease cleavable Strep tag in E. coli

   Depositor

   Justin Kollman

   Insert

   Nsp14-TEV-Strep (ORF1ab SARS-CoV-2)
   

   Tags

   TEV-Strep

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pHMM

   Plasmid

   #67717

   Purpose

   Expression vector MBP/hexahistidine tagged variant of the MS2 coat protein

   Depositor

   Jeffrey Kieft, Robert Batey

   Article

   Batey et al RNA. 2007 Aug;13(8):1384-9. Epub 2007 Ju

   Insert

   Hexahistidine/Maltose binding protein/MS2 coat protein fusion
   

   Tags

   Hexahistidine tag

   Expression

   Bacterial

   Promoter

   T7 RNA polymerase

   Available Since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pACM4

    Plasmid

    #49797

    Purpose

    ePathBrick vector for pathway engineering in E. coli.

    Depositor

    Mattheos Koffas

    Article

    Xu et al ACS Synth Biol. 2012 Jul 20;1(7):256-66.

    Type

    Empty backbone

    Tags

    S-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLJSRSF3

    Plasmid

    #64692

    Purpose

    His-GST-Smt3-NpuDnaEN102-CBD

    Depositor

    Hideo Iwai

    Article

    Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

    Type

    Empty backbone

    Tags

    CBD, GST, HisTag, NpuDnaE-N102, and Smt3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHMGWA-Pa14Csy4H29A

    Plasmid

    #41092

    Depositor

    Jennifer Doudna

    Article

    Haurwitz et al Science. 2010 Sep 10;329(5997):1355-8.

    Insert

    Csy4
    

    Tags

    His6-MBP

    Mutation

    His29Ala mmutation to abolish ribonuclease activi…

    Promoter

    T7

    Available Since

    Dec. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

13. GST-thr-RAF1-RBD

    Plasmid

    #86033

    Purpose

    Expresses the RAS-binding domain of RAF1 for in vitro analysis of RAS activation

    Depositor

    Dominic Esposito

    Insert

    RAF1 RBD (Ras-binding domain) (RAF1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Contains residues 1-149

    Promoter

    T7

    Available Since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHN1002

    Plasmid

    #178831

    Purpose

    Bacterial expression for human Saposin A

    Depositor

    Kazuhiro Abe

    Insert

    artificial gene encoding human Saposin A (AR Human)
    

    Tags

    His8, 3C

    Expression

    Bacterial

    Mutation

    Codon-usage was optimized for bacterial expressio…

    Promoter

    T7

    Available Since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. SIRT5-pET15b

    Plasmid

    #81030

    Purpose

    Bacterial expression of Human WT SIRT5 protein

    Depositor

    David Lombard

    Article

    Kumar et al Methods Mol Biol. 2016;1436:241-57. doi:

    Insert

    Sirt5 (SIRT5 Human)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. 18 pCOLI_A_Trx

    Plasmid

    #160255

    Purpose

    N-term His-Thioredoxin 3C cleavable Amp resistant bacterial expression vector

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Tags

    6xHis and Thioredoxin

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDM4

    Plasmid

    #49796

    Purpose

    ePathBrick vector for pathway engineering in E. coli.

    Depositor

    Mattheos Koffas

    Article

    Xu et al ACS Synth Biol. 2012 Jul 20;1(7):256-66.

    Type

    Empty backbone

    Tags

    S-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. 23 pCOLI_A_N-SUMO_C_STREP

    Plasmid

    #160259

    Purpose

    N-term His-SUMO 3C cleavable, C--term 2xStrepII Amp resistant bacterial expression vector

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Tags

    2xStrepII, 6xHis, and SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRSM3

    Plasmid

    #49799

    Purpose

    ePathBrick vector for pathway engineering in E. coli.

    Depositor

    Mattheos Koffas

    Article

    Xu et al ACS Synth Biol. 2012 Jul 20;1(7):256-66.

    Type

    Empty backbone

    Tags

    S-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. 3CEG

    Plasmid

    #60107

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    BIRC6:SDC136-F10:C41228 (BIRC6 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available Since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits