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Showing: 5481 - 5520 of 10096 results

  2. pAC1-pCR8-dCas9VP48

    Plasmid
    #48214
    Purpose
    dCas9VP48 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression
    Depositor
    Rudolf Jaenisch
    Article
    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2
    Insert
    dCas9(D10A;H840A) fusion with VP48 activation domain
    Use
    CRISPR; Gateway donor
    Tags
    HA Tag and VP48
    Expression
    Mutation
    D10A;H840A
    Promoter
    Available since
    Sept. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    48214-plasmid-map-sequence-id-69493

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  25. p406 – TDH3-ER-FlucDM-GFP11-Ura

    Plasmid
    #177731
    Purpose
    DM Firefly luciferase reporter fused to a KAR2 signal sequence for ER targeting and HDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminus in yeast plasmid with
    Depositor
    Erik Böttger
    Article
    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:
    Insert
    FlucDM
    Use
    Tags
    GFP11, HA, HDEL, and KAR2 Signal Sequence
    Expression
    Bacterial and Yeast
    Mutation
    R188Q, R261Q (Destabilized Mutant)
    Promoter
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    177731-plasmid-map-sequence-id-352614

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  28. IX907: pMVP (L3-L2) 3x epitope tag + WPRE

    Plasmid
    #121757
    Purpose
    pMVP L3-L2 entry plasmid, contains 3x HA epitope tag + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest in lentivirus vectors.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    3x HA epitope tag + WPRE
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121757-plasmid-map-sequence-id-237237

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  31. pFRT-TODestFLAGHA_RBPMS_KDR_111del

    Plasmid
    #65659
    Purpose
    FLAG-HA expression vector with RBPMS carrying deletion at amino acid 111 (1-111) and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)
    Depositor
    Thomas Tuschl
    Article
    Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.
    Insert
    RBPMS (RBPMS Human)
    Use
    Tags
    FLAG HA
    Expression
    Mammalian
    Mutation
    introduced stop codon such as to produce deletion…
    Promoter
    CMV, 2x Tet
    Available since
    June 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    65659-plasmid-map-sequence-id-111636

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  35. pENTR11 WT NFAT1

    Plasmid
    #11791
    Depositor
    Anjana Rao
    Insert
    NFAT1 (Nfatc2 Mouse)
    Use
    Tags
    HA
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    11791-plasmid-map-sequence-id-7060

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  37. pSL24a-Linker-smHA-FP-DHFR-LoxP

    Plasmid
    #166238
    Purpose
    Tagging of weakly expressed genes, floxed DHFR marker
    Depositor
    David Sibley
    Insert
    Spaghetti monster HA
    Use
    Tags
    Expression
    Mutation
    none
    Promoter
    Available since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    166238-plasmid-map-sequence-id-329831

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  38. pUS232

    Plasmid
    #84579
    Purpose
    Shuttle and expression vector. Predicted to replicate in many Gram-negative bacteria. Has multiple cloning site with blue/white selection function. Tetracycline-inducible expression of cloned genes.
    Depositor
    Nicholas Coleman
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 21, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    84579-plasmid-map-sequence-id-217782

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  39. pShuttle AMPK gamma1 H150R

    Plasmid
    #15999
    Depositor
    Morris Birnbaum
    Insert
    AMPK gamma1 (Prkag1 Mouse)
    Use
    Adenoviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    H150R
    Promoter
    Available since
    Oct. 26, 2007
    Availability
    Academic Institutions and Nonprofits only

  40. pAAV-CAG-Flex-Rev-3xGFP

    Plasmid
    #122500
    Purpose
    Cre dependent GFP expression.
    Depositor
    Kimberly Ritola
    Insert
    3x GFP
    Use
    AAV
    Tags
    Expression
    Mutation
    each GFP has unique nt sequence
    Promoter
    Available since
    Jan. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    122500-plasmid-map-sequence-id-237802

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Showing: 5481 - 5520 of 10096 results