We narrowed to 8,624 results for: FIE

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EGFP-uTEV1-NES

Plasmid

#171006

Purpose

HiLITR protease with C-terminal NNES (Cytoplasmic)

Depositor

Alice Ting

Article

Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

Insert

EGFP-uTEV1-NES

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

pTRE-tight (TetOn)

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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p1192-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIC3Nme_FLAG_NLS

Plasmid

#129530

Purpose

Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIC3Nme

Depositor

Erik Sontheimer

Article

Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

Inserts

codon-optimized AcrIIC3Nme
sgRNA_Rosa26

Use

AAV

Tags

FLAG/NLS

Promoter

U6 and cytomegalovirus-enhancer chicken β-actin p…

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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FEV_pet28a

Plasmid

#131668

Purpose

Bacterial expression of ETS gene FEV

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

FEV (FEV Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pLXSN-H-Ras_N17

Plasmid

#39537

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS N17 (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

N17

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
RHBDD1 pmRFP-N2 (1390)

Plasmid

#62049

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

RHBDD1 (RHBDD1 Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pLEX Myc-HA

Plasmid

#229499

Purpose

For lentiviral expression of Myc coding sequence with an HA tag.

Depositor

Davide Ruggero

Article

Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/

Insert

MYC

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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ETV1_pet28a

Plasmid

#131644

Purpose

Bacterial expression of ETS gene ETV1

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ETV1 (ETV1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry-7aa-ActA-pEGFP-C1

Plasmid

#177406

Purpose

To express mCherry fused to mitochondria outer membrane targeting sequence "ActA". mCherry faces the cytosol.

Depositor

David Andrews

Article

Aranovich et al Mol Cell. 2012 Mar 30;45(6):754-63. doi:

Insert

ActA tail anchor (actA Listeria monocytogenes)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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RHBDD1 pEGFP-N2 (1078)

Plasmid

#62028

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

RHBDD1 (RHBDD1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only

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ETS2_pet28a

Plasmid

#131664

Purpose

Bacterial expression of ETS gene ETS2

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ETS2 (ETS2 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pDest-636

Plasmid

#159574

Purpose

Gateway Destination vector for protein expression in baculovirus

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Type

Empty backbone

Tags

His6-MBP

Expression

Insect

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pX330_ATP1A1_G7_Dual_sgRNA

Plasmid

#173206

Purpose

Coselection for HDR in human cells. Vector for dual expression of ATP1A1 G7 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G7 sgRNA + user-specified sgRNA + SpCas9

Use

CRISPR

Expression

Mammalian

Promoter

Dual U6 promoters

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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p1194-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIA4_FLAG_NLS

Plasmid

#129532

Purpose

Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIA4

Depositor

Erik Sontheimer

Article

Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

Inserts

codon-optimized AcrIIA4
sgRNA_Rosa26

Use

AAV

Tags

FLAG/NLS

Promoter

U6 and cytomegalovirus-enhancer chicken β-actin p…

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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ELF4_pet28a

Plasmid

#131661

Purpose

Bacterial expression of ETS gene ELF4

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ELF4 (ELF4 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pEasyG1_hph

Plasmid

#184912

Purpose

Template to generate via PCR a single gRNA for expression in S. cerevisiae

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial and Yeast

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD02_CBASS_Escherichia_coli_CVM_N37067PS

Plasmid

#224403

Purpose

For expression of the type I CBASS system encoded by Escherichia coli CVM N37067PS

Depositor

Philip Kranzusch

Article

Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

Insert

EcCdnF_AGS-C_SLATT6

Expression

Bacterial

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD02_CBASS_Escherichia_coli_BIDMC103

Plasmid

#224402

Purpose

For expression of the type I CBASS system encoded by Escherichia coli BIDMC103

Depositor

Philip Kranzusch

Article

Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

Insert

EcCdnF_AGS-C_SLATT6

Expression

Bacterial

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD02_CBASS_Escherichia_coli_K71-77

Plasmid

#224398

Purpose

For expression of the type I CBASS system encoded by Escherichia coli K71-77

Depositor

Philip Kranzusch

Article

Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

Insert

Phospholipase_EcCdnE_DisA-like

Expression

Bacterial

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD02_CBASS_Escherichia_coli_RU1_BHI6

Plasmid

#224397

Purpose

For expression of the type I CBASS system encoded by Escherichia coli RU1 BHI6

Depositor

Philip Kranzusch

Article

Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

Insert

Cap15_EcCdnE

Expression

Bacterial

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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EGFP-uTEV1-MAVS

Plasmid

#170998

Purpose

HiLITR protease with MAVS c-terminal tmd targeting information (Mitochondria)

Depositor

Alice Ting

Article

Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

Insert

EGFP-uTEV1-MAVS(tmd)

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

pTRE-tight (TetOn)

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge