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dCas9-NONO2_IDR-EGFP

Plasmid

#232765

Purpose

Expresses dCas9-NONO2_IDR-EGFP

Depositor

Isaac Hilton

Article

Li et al Nucleic Acids Res. 2025 Feb 8;53(4):gkaf

Insert

dCas9-NONO2_IDR-EGFP (NONO Human)

Use

Lentiviral

Promoter

EF1a

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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dCas9-NONO1_IDR-EGFP

Plasmid

#232764

Purpose

Expresses dCas9-NONO1_IDR-EGFP

Depositor

Isaac Hilton

Article

Li et al Nucleic Acids Res. 2025 Feb 8;53(4):gkaf

Insert

dCas9-NONO1_IDR-EGFP (NONO Human)

Use

Lentiviral

Promoter

EF1a

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLIX401 APE1 CI

Plasmid

#227191

Purpose

Inducible lentiviral expression of CI (Catalytically Inactive) APE1

Depositor

David Pellman

Article

Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

Insert

apurinic/apyrimidinic endonuclease APE1 (APEX1 Human)

Use

Lentiviral

Expression

Bacterial and Mammalian

Mutation

H309N

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pLIX401 APE1 SO

Plasmid

#227192

Purpose

Inducible lentiviral expression of SO (Slow Off) APE1

Depositor

David Pellman

Article

Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

Insert

apurinic/apyrimidinic endonuclease APE1 (APEX1 Human)

Expression

Bacterial and Mammalian

Mutation

D283A D308A

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pAf-CRISPR-ctfR1

Plasmid

#207992

Purpose

CRISPR vector used with pAf-CRISPR-phoA (#207991) to delete both aflatoxin and cyclopiazonic acid gene clusters of Aspergillus flavus

Depositor

Perng-Kuang Chang

Article

Chang et al Fungal Genet Biol. 2023 Dec 27;170:10386

Insert

ctfR1

Use

CRISPR

Promoter

Aspergillus flavus U6

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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H2B-mNeonGreen_FRB(DmrC)_IRESpuro2

Plasmid

#182918

Purpose

Expesses H2B-mNeonGreen_FRB(DmrC). Can be used to target FKBP(DmrA) tagged proteins to H2B

Depositor

Daniel Gerlich

Article

Cuylen-Haering et al Nature. 2020 Nov;587(7833):285-290. doi:

Insert

pH2B (H2BC16P Human)

Tags

mNeonGreen-FRB(DmrC)

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pRecomb-loxP-mCherry-4Gcloningsite-lox2272-66

Plasmid

#168787

Purpose

Donor vector for recombinase mediated integration in filamentous fungi. Is a shuttle vector for optional in vivo cloning in yeast, and contains four inducible promoters or sites for whole BGC cloning.

Depositor

Yit Heng Chooi

Article

Roux et al ACS Synth Biol. 2022 Feb 16. doi: 10.102

Type

Empty backbone

Use

Cre/Lox and Synthetic Biology; Expression in fila…

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pPB-tetpA(R1R4)-iRPT

Plasmid

#154882

Purpose

Ubiquitously expresses iRFP713, Puromycin resistance gene, and rtTAM2.

Depositor

Hideyuki Okano

Article

Nemoto et al Cells. 2020 Dec 22;10(1). pii: cells1001

Inserts

iRFP713
Puromycin resistance gene
rtTAM2

Expression

Mammalian

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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Ir7a-T2A-QF2 HDR plasmid

Plasmid

#140943

Purpose

Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti Ir7a gene

Depositor

Leslie Vosshall

Article

Jove et al Neuron. 2020 Dec 23;108(6):1163-1180.e12

Insert

Ir7a-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir7a-right-HDR-arm

Available Since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pLL1272

Plasmid

#129287

Purpose

Expression plasmid with pLL1270 as backbone and alpha-Xylp_1211 and alpha-L-Araf_1120 as inserts

Depositor

Lee Lynd

Inserts

alpha-Xylp_1211 (Ga0256695_ 1211)
alpha-L-Araf_1120

Expression

Bacterial

Promoter

Clostridium thermocellum DSM1313 Cellobiose Phosp…

Available Since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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pJET1.2_Bod1l_Sense

Plasmid

#124443

Purpose

Plasmid for sense in situ probe in vitro transcription

Depositor

Yingguang Frank Chan

Article

Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

Insert

Biorientation of chromosomes in cell division 1-like (Bod1l Mouse)

Use

In situ probe

Promoter

T7

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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pN_35S/CURT_flouB

Plasmid

#117995

Purpose

Expression of CURT_flouB controlled by the CaMV 35S promoter

Depositor

Mathias Pribil

Article

Behrendorff et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

CURT_flouB

Use

Yeast/bacteria binary vector

Expression

Plant

Promoter

Cauliflower mosaic virus 35S

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCR3-vsv-CenpB 1-166

Plasmid

#108474

Purpose

expression of vsv-CenpB 1-166

Depositor

Susanne Lens

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

CenpB 1-166 AA (CENPB Human)

Tags

VSV

Expression

Mammalian

Mutation

encodes only the DNA-binding domain of CENP-B

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3-vsv-Mis12-dCEN-Incenp

Plasmid

#108477

Purpose

expression of vsv-Mis12-dCEN-INCENP

Depositor

Susanne Lens

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

Mis12-dCEN-INCENP (INCENP Human)

Tags

VSV

Expression

Mammalian

Mutation

CEN box replaced by Mis12

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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B3GALT5_pCSdest

Plasmid

#53941

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

B3GALT5 (B3GALT5 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
C21orf63_pCSdest

Plasmid

#53857

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

EVA1C (EVA1C Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
DSCR8_pCSdest

Plasmid

#53828

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

DSCR8 (DSCR8 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

Sept. 9, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pJB008

Plasmid

#22647

Depositor

Lynn Hudson

Article

Hudson et al Proc Natl Acad Sci U S A. 1989 Oct . 86(

Insert

Human genomic PLP (PLP1 Human)

Use

Phagemid

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only
pYTSK56K_3G7

Plasmid

#177301

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn7 with GmR and mCherry

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

mCherry

Use

Synthetic Biology; Yeast expression, tn7 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pYTSK58K_6G7

Plasmid

#177302

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn7 with GmR and PE-H

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

pe-h

Use

Synthetic Biology; Yeast expression, tn7 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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