We narrowed to 4,074 results for: p2a

1. SECURE miniABEmax-V82G (pJUL1828)

   Plasmid

   #131313

   Purpose

   CMV promoter expression plasmid for bpNLS-TadA7.10(V82G)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with V82G mutation).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

   Insert

   bpNLS-TadA7.10(V82G)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDIRECT_26H

   Plasmid

   #91150

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:bar

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMXs2-RAC1

   Plasmid

   #86138

   Purpose

   Retrolviral vector expressing RAC1-IRES-Bsd-p2A-RFP

   Depositor

   David Sabatini

   Article

   Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

   Insert

   RAC1 (RAC1 Human)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

4. pFETCH_ATF1

   Plasmid

   #64689

   Purpose

   Homology Arms and 3X Flag with P2A Neo for 3' tagging of human ATF1

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   ATF1 Homology Arms (ATF1 Human)
   

   Use

   Tags

   3X Flag P2A Neo

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-VTKS5-TVA-eGFP-N2cG

   Plasmid

   #175440

   Purpose

   Cre-on / Flp-off helpers for N2c rabies monosynaptic tracing; TVA, eGFP and N2cG under Synpasin promotoer

   Depositor

   Gordon Fishell

   Article

   Pouchelon et al Cell Rep. 2021 Nov 9;37(6):109993. doi:

   Insert

   TVA-P2A-eGFP-P2A-N2cG
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDIRECT_25I

   Plasmid

   #91146

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9_dead + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:hpt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9_dead + PvUbi1:gRNAs with Csy4 spacers
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D10A, H840A

   Promoter

   Available since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. JPF0454

   Plasmid

   #124039

   Purpose

   Encodes pPGK1 driving expression of spCas9 P2A mAzamiGreen in a 2nd gen. Lenti Virus destination vector

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   pHR_pPGK1-spCAS9-P2A-mAzamiGreen
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB758

   Plasmid

   #210033

   Purpose

   Contains inducible Cas7-P2A-Cas5-T2A-Cas8, rtTA-T2A-puro

   Depositor

   Michael Talkowski

   Article

   Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

   Inserts

   Cas7-P2A-Cas5-T2A-Cas8
   rtta-T2A-puro
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α and TRE3G

   Available since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTS2361_Tier3(SB)-rtTA-YPet_PuroR

   Plasmid

   #169657

   Purpose

   Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a YPet marker and PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PRPBSA

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTS2363_Tier3(PB)-rtTA-YPet_PuroR

    Plasmid

    #169659

    Purpose

    Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a YPet marker and PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PRPBSA

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJET-col2a1a-KI-donor

    Plasmid

    #184876

    Purpose

    Donor template for knock-in of p2a-CreERT2 into the medaka col2a1a locus via CRISPR/Cas9-mediated homology directed repair

    Depositor

    Christoph Winkler

    Article

    Hui et al Development. 2022 May 20. pii: 275483. d

    Inserts

    Collagen2a1a 5' homology arm
    Collagen2a1a 3' homology arm
    p2a-CreERT2
    Myosin light chain 2 promoter
    EGFP
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDH-TRE3G-N-tdT-CMV-P

    Plasmid

    #192887

    Purpose

    Tet-inducible Nanoluc-tdTomato expression

    Depositor

    Kazuhiro Oka

    Insert

    Nanoluc-P2A-tdTomato
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCDH-TRE3G-N-cG-CMV-P

    Plasmid

    #192886

    Purpose

    Tet inducible Nanoluc copGFP expression

    Depositor

    Kazuhiro Oka

    Insert

    Nanoluc-P2A-copGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AV0.1-CWS-Cre-tdT

    Plasmid

    #192876

    Purpose

    coexpression of Cre and tdT

    Depositor

    Kazuhiro Oka

    Insert

    Cre-P2A-tdTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDH-EF1-DIO-Nluc-copGFP-IRES-Puro

    Plasmid

    #192875

    Purpose

    Cre-dependent dual reporter

    Depositor

    Kazuhiro Oka

    Insert

    Nanoluc-P2A-copGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCDH-CMV5-N-cG-P

    Plasmid

    #192883

    Purpose

    coexpression of Nanoluc, copGFP and puromycin resistant marker

    Depositor

    Kazuhiro Oka

    Insert

    Nanoluc-P2A-copGFP-T2A-Puro
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPD1025 EF1alpha TetON3G in TU6

    Plasmid

    #139262

    Purpose

    Expresses TetON3G under hEF1alpha in TUPV6

    Depositor

    Joshua Leonard

    Insert

    TetON3G
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hEF1alpha

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPD1026 EF1alpha TetON3G in TU2

    Plasmid

    #139258

    Purpose

    Expresses TetON3G under hEF1alpha in TUPV2

    Depositor

    Joshua Leonard

    Insert

    TetON3G
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hEF1alpha

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPD1024 EF1alpha TetON3G in TU5

    Plasmid

    #139261

    Purpose

    Expresses TetON3G under hEF1alpha in TUPV5

    Depositor

    Joshua Leonard

    Insert

    TetON3G
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hEF1alpha

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJM469 EF1alpha TetON3G in TU4

    Plasmid

    #139260

    Purpose

    Expresses TetON3G under hEF1alpha in TUPV4

    Depositor

    Joshua Leonard

    Insert

    TetON3G
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hEF1alpha

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge