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pCS2-foxd4l1.1-deltaRII-Cterm-myc

Plasmid

#185505

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

myc

Expression

Mutation

RII domain and C-term deleted

Promoter

Available since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-deltaRII-Cterm

Plasmid

#185504

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

Expression

Mutation

RII domain and C-term deleted

Promoter

Available since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-deltaN-myc

Plasmid

#185500

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

myc

Expression

Mutation

N-terminus deleted

Promoter

Available since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-deltaC-myc

Plasmid

#185501

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

myc

Expression

Mutation

C-terminus deleted

Promoter

Available since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-A6-myc

Plasmid

#185507

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

myc

Expression

Mutation

FSIENIM to AAAAAAM

Promoter

Available since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-A6

Plasmid

#185506

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

Expression

Mutation

FSIENIM to AAAAAAM

Promoter

Available since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pUG36-NES-EGFP-cPHx3

Plasmid

#183668

Purpose

PtdIns(3,4)P2 biosensor for expression in yeast

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

PLEKHA1 (PLEKHA1 Frog, Human)

Use

Tags

X. laevis map2k1.L(32-44)-EGFP

Expression

Yeast

Mutation

Amino acids 169-329 (tandem trimer)

Promoter

MET25

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pUG34-NES-EGFP-cPHx3

Plasmid

#183669

Purpose

PtdIns(3,4)P2 biosensor for expression in yeast

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

PLEKHA1 (PLEKHA1 Frog, Human)

Use

Tags

X. laevis map2k1.L(32-44)-EGFP

Expression

Yeast

Mutation

Amino acids 169-329 (tandem trimer)

Promoter

MET25

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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NES-mCherry-PH-BTKx1

Plasmid

#183653

Purpose

PtdIns(3,4,5)P3 biosensor

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

BTK (BTK Frog, Human)

Use

Tags

X. laevis map2k1.L(32-44)-mCherry

Expression

Mammalian

Mutation

Amino acids 2-170

Promoter

CMV

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-Bighead S-FC

Plasmid

#180147

Purpose

mRNA synthesis for injection into embryos

Depositor

Edward De Robertis

Article

Ding et al Proc Natl Acad Sci U S A. 2018 Sep 25;11

Insert

Bighead.S

Use

Tags

FC

Expression

Mammalian

Mutation

Promoter

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2+WBP2NL-HA

Plasmid

#174791

Purpose

Xenopus laevis WBP2NL with 3'HA tag

Depositor

Sally Moody

Article

Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

Insert

WBP2 N-terminal like (wbp2nl.L Frog)

Use

Tags

HA

Expression

Bacterial

Mutation

aa#165, Y to H; aa# 287, D to H

Promoter

SP6

Available since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+-wbp2nlY282F

Plasmid

#172468

Purpose

expresses protein with one aa substitution to affect potential phosphorylation in WW-interaction domain

Depositor

Sally Moody

Article

Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

Insert

wbp2nlY282F

Use

Xenopus expression

Tags

none

Expression

Mutation

changed Tyrosine at aa282 to Phenylalanine

Promoter

SP6

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+-wbp2nlF127P

Plasmid

#174507

Purpose

expresses protein with one aa substitution to disrupt the alpha-helix near the PH-G domain

Depositor

Sally Moody

Article

Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

Insert

wbp2nlF127P

Use

Xenopus expression

Tags

none

Expression

Mutation

changed Phenylalanine at aa127 to Proline

Promoter

SP6

Available since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+-wbp2nlT45A

Plasmid

#172464

Purpose

expresses protein with one aa substitution to affect potential phosphorylation

Depositor

Sally Moody

Article

Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

Insert

wbp2nlT45A

Use

Xenopus expression

Tags

none

Expression

Mutation

changed Threonine at aa45 to Alanine

Promoter

SP6

Available since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+-wbp2nlY55F

Plasmid

#172466

Purpose

expresses protein with one aa substitution to affect potential phosphorylation in ITSM domain

Depositor

Sally Moody

Article

Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

Insert

wbp2nlY55F

Use

Xenopus expression

Tags

none

Expression

Mutation

changed Tyrosine at aa55 to Phenylalanine

Promoter

SP6

Available since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+-wbp2nl

Plasmid

#172463

Purpose

expresses wild-type protein when injected into embryos

Depositor

Sally Moody

Article

Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

Insert

wbp2nl (wbp2nl.L Frog)

Use

Xenopus expression

Tags

none

Expression

Mutation

none

Promoter

SP6

Available since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-Xlrbpa-dsRBD(+6)

Plasmid

#171548

Purpose

Encoding part of the Xenopus Xlrbpa-2 protein from M120 to L202

Depositor

Yan Zeng

Article

Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

Xlrbpa (prkra.L Frog)

Use

Tags

Expression

Bacterial

Mutation

a six amino acid_TRLTEG_insertion in the L1 region

Promoter

Available since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-Xlrbpa

Plasmid

#171547

Purpose

Encoding part of the Xenopus Xlrbpa-2 protein from M120 to L202

Depositor

Yan Zeng

Article

Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

Xlrbpa (prkra.L Frog)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-xFAM3b.L-HA

Plasmid

#172852

Purpose

mRNA synthesis for injection into embryos

Depositor

Edward De Robertis

Article

Ding et al Proc Natl Acad Sci U S A. 2018 Sep 25;11

Insert

xFAM3B.L (fam3b.L Frog)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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KSP_HNL

Plasmid

#129772

Purpose

Expresses Xenopus Kinesin-5 KSP motor and neck linker domain monomer for biochemical assays

Depositor

William Hancock

Insert

KSP (kif11.L Frog)

Use

Tags

His6

Expression

Bacterial

Mutation

Truncation at 367

Promoter

Available since

Dec. 11, 2019

Availability

Academic Institutions and Nonprofits only

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