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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 24,010 results for: Nes

Showing: 391 - 420 of 24010 results

  1. pcDNA3-JNKsub(TA)DD-YPet-NES

    Plasmid
    #84634
    Purpose
    Encodes negative-control C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    JNKsub(TA)DD-YPet-NES
    Use
    Tags
    6xHis, Nuclear export signal (NES), T7 tag (gene …
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available since
    Nov. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    84634-plasmid-map-sequence-id-176198

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  4. pcDNA3-ERKsub(TA)DD-Venus-NES

    Plasmid
    #84631
    Purpose
    Encodes negative-control C-terminal (substrate) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    ERKsub(TA)DD-Venus-NES
    Use
    Tags
    6xHis, Nuclear export signal (NES), T7 tag (gene …
    Expression
    Mammalian
    Mutation
    Mutated target Thr residue to Ala.
    Promoter
    CMV
    Available since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    84631-plasmid-map-sequence-id-175151

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  5. pcDNA3-PKCsub(T/A)-YPet-NES

    Plasmid
    #138208
    Purpose
    Encodes negative-control C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    PKCsub(T/A)-YPet-NES
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available since
    Sept. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    138208-plasmid-map-sequence-id-307411

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  6. pcDNA3-RLuc8C-FHA1-NES

    Plasmid
    #138210
    Purpose
    Encodes PAABD fragment of luminescence-based bimolecular kinase activity reporters (LumKARs); cytosol targeted.
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    RLuc8C-FHA1-NES
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    138210-plasmid-map-sequence-id-307412

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  11. pCMV-dCas13-NES-eNAT10

    Plasmid
    #238299
    Purpose
    Expresses cytoplasmic version of the programmable RNA acetylation system.
    Depositor
    Won Do Heo
    Article
    Yu et al Nat Chem Biol. 2025 Jun 2. doi: 10.1038/
    Insert
    dPspCas13b fused to eNAT10 (NAT10 Prevotella sp. P5-125, Human)
    Use
    CRISPR
    Tags
    HIV NES (C terminal of dPspCas13b)
    Expression
    Mammalian
    Mutation
    H133A for catalytically inactive mutant, deletion…
    Promoter
    Available since
    June 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    238299-plasmid-map-sequence-id-472414

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  13. pAAV_hSyn_NES-Kinprola_on-mEGFP_WRPE-SV40

    Plasmid
    #233368
    Purpose
    hSyn1 driven expression of the consitutively active kinase activity recorder positive control Kinprola_on fused to mEGFP for neuronal expression through AAV transduction
    Depositor
    Kai Johnsson
    Article
    Sun et al bioRxiv 2024.09.11.611894
    Insert
    NES-Kinprola_on-mEGFP
    Use
    AAV
    Tags
    NES and mEGFP
    Expression
    Mutation
    Promoter
    hSyn1
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    233368-plasmid-map-sequence-id-466650

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  14. pAAV_hSyn_NES-Kinprola_PKA_T/A-mEGFP_WRPE-SV40

    Plasmid
    #233367
    Purpose
    hSyn1 driven expression of the inactive PKA activity recorder negative control with T/A mutation Kinprola_PKA fused to mEGFP for neuronal expression through AAV transduction
    Depositor
    Kai Johnsson
    Article
    Sun et al bioRxiv 2024.09.11.611894
    Insert
    NES-Kinprola_PKA_T/A-mEGFP
    Use
    AAV
    Tags
    NES and mEGFP
    Expression
    Mutation
    Promoter
    hSyn1
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    233367-plasmid-map-sequence-id-466644

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  21. pCDNA5/FRT_CMV_NES-Kinprola_on-mEGFP

    Plasmid
    #233355
    Purpose
    CMV driven expression of the consitutively active kinase activity recorder positive control Kinprola_on in mammalian cell lines fused to mEGFP
    Depositor
    Kai Johnsson
    Article
    Sun et al bioRxiv 2024.09.11.611894
    Insert
    NES-Kinprola_on-mEGFP
    Use
    Tags
    NES and mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    233355-plasmid-map-sequence-id-466631

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  26. pLKO.1-puro_CMV_NES-Kinprola_on-mEGFP

    Plasmid
    #233370
    Purpose
    CMV driven expression of the consitutively active kinase activity recorder positive control Kinprola_on fused to mEGFP for neuronal expression through lentivirus transduction
    Depositor
    Kai Johnsson
    Article
    Sun et al bioRxiv 2024.09.11.611894
    Insert
    NES-Kinprola_on-mEGFP
    Use
    Lentiviral
    Tags
    NES and mEGFP
    Expression
    Mutation
    Promoter
    CMV
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    233370-plasmid-map-sequence-id-466616

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Showing: 391 - 420 of 24010 results