We narrowed to 6,763 results for: poly
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Plasmid#159496PurposeTests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with an immediately upstream hairpin have on human polymerase III transcription elongation from a U6 promoter.DepositorInsertTermination Module:Linear-1_PolyU(4) _HairpinDistance(0)
ExpressionMammalianPromoterU6-27Available SinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Linear-1_PolyU(1)_HairpinDistance(0)nts_Tornado-Corn
Plasmid#159495PurposeTests for the impacts 1 uracil in a poly-uracil tract in conjunctiuon with an immediately upstream hairpin have on human polymerase III transcription elongation from a U6 promoter.DepositorInsertTermination Module:Linear-1_PolyU(1)_HairpinDistance(0)nts
ExpressionMammalianPromoterU6-27Available SinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
IX702: pMVP (L3-L2) OLLAS-IRES-eGFP + polyA
Plasmid#121748PurposepMVP L3-L2 entry plasmid, contains OLLAS epitope tag + IRES2-eGFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion and IRES expression of eGFP reporterDepositorInsertOLLAS epitope tag-IRES2-eGFP + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IX503: pMVP (L3-L2) OLLAS epitope tag + polyA
Plasmid#121752PurposepMVP L3-L2 entry plasmid, contains OLLAS epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.DepositorInsertOLLAS epitope tag + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IX401: pMVP (L3-L2) 6x His epitope tag + polyA
Plasmid#121754PurposepMVP L3-L2 entry plasmid, contains 6x His epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.DepositorInsert6x His epitope tag + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-5'Luciferase(split CAGGT)_BDlacZ-SV40polyA
Plasmid#216320PurposeSplit luciferase assay to test reconstitution via mRNA trans-splicing (REVeRT system).DepositorInsertSplit Luciferase + splice donor site
UseAAVPromoterCMVAvailable SinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-TRE-Cas9- puro-polyA-CAG-rtTA
Plasmid#107270PurposeExpresses Cas9 upon induction with doxicycline.DepositorInsertshSpCas9
rtTA
bGH polyA-SV40polyA
Tags3xFlag, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianPromoterCAG and TREAvailable SinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGP-CAG-iGABASnFR2(no bind)-WPRE-bGH-polyA
Plasmid#218870PurposeMammalian expression of improved GABA sensor control (no change in fluorescence)DepositorInsertiGABASnFR2(no bind)
ExpressionMammalianMutationS99A F102G R168PPromoterCAGAvailable SinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
L4802 VEGFR2 NTEVp chain (WT NTEVp) in PolyTX-mNeonGreen
Plasmid#244173PurposeConstitutive expression of a MESA NTEVp chain encoding (N to C): VEGFR2SS-3xFLAG-VEGFR2ECD-VEGFR2TMD-NTEVp(WT)DepositorInsertMESA NTEVp chain with human VEGFR2 SS, ECD and TMD, and NTEVp (WT) (KDR Human, Synthetic)
UseSynthetic BiologyTagsVEGFR2 signal peptide - 3xFLAGExpressionMammalianPromoterhEF1aAvailable SinceOct. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-hCHD7-Kozak ATG 3' 3x Flag-bGH polyA
Plasmid#89459Purposeconstitutive expression of human CHD7 in mammalian cellsDepositorAvailable SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC19-TgKI-MCS-p2A-mScarlet-MCS-polyA
Plasmid#174029PurposeThis plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.DepositorInsertp2A-mScarlet
UseZebrafish knock-in taggingAvailable SinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSF4 CMV intron renilla TRICK CTE polyA
Plasmid#84443PurposeTRICK reporter mRNADepositorInsertRenill-TRICK
UseLuciferase; FrtExpressionMammalianPromoterTet-CMVAvailable SinceNov. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
8xGliBS-IVS2-mCherry-NLS-Odc1-polyA-Tol2
Plasmid#84603PurposeTol2-based hedgehog signal reporter plasmid for zebrafish transgenesisDepositorInsertmCherry
UseZebrafish transgenesisTagsODC1 destabilizing elementPromoter8xGliBS-delta-crystallin minimal promoterAvailable SinceOct. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC19-TgKI-MCS-p2A-eGFP-MCS-polyA
Plasmid#174028PurposeThis plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.DepositorInsertp2A-eGFP
UseZebrafish knock-in taggingAvailable SinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-CAG-VARNAM A122D WPRE-bGH-polyA
Plasmid#180486PurposeMammalian expression of voltage sensorDepositorInsertVARNAM A122D
ExpressionMammalianMutationA122DPromoterCAGAvailable SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRII-TOPO CMV-cGFP-bGH Poly(A) Sense
Plasmid#46835PurposeExpress cGFP transcript ending in a poly(A) tail [using the bGH poly(A) signal]DepositorInsertCoral Green Fluorescent Protein (cGFP)
ExpressionMammalianPromoterCMVAvailable SinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCRII-TOPO CMV-cGFP-SV40 Poly(A) Sense
Plasmid#46836PurposeExpress cGFP transcript ending in a poly(A) tail [using the SV40 poly(A) signal]DepositorInsertCoral Green Fluorescent Protein (cGFP)
ExpressionMammalianPromoterCMVAvailable SinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
Recruited polymerase expression - pCMV-T7-ePhi29(+exo)-CC(N5) (LM2982)
Plasmid#208972PurposeRecruited ePhi29(+exo) DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.DepositorInsertePhi29(+exo)-BPNLS-CC(N5)
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationePhi29(+exo)(M8R/V51A/M97T/G197D/E221K/Q497P/K512…PromoterCMV and T7Available SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-EGFPnls barcode-206-SV40 polyA
Plasmid#190875PurposeFor barcoded retrograde labelingDepositorInsertEGFPnls-barcode206
UseAAVMutationWTAvailable SinceAug. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-G1B3-Mito-Timer-WPRE-miR124T-SYNpolyA
Plasmid#245076PurposeMito-Timer fluorescent reporter, with miRNA-124 target sequence to repress transgene expression in neuronsDepositorInsertmito-Timer
UseAAVExpressionMammalianPromoterHuman G1B3 promoterAvailable SinceOct. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynI-mCherry-WPRE-hGH poly(A) (Alex_01)
Plasmid#71650PurposeAAV expression of mCherry in human and mouse neuronsDepositorInsertmCherry
UseAAVExpressionMammalianPromoterhSynIAvailable SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynI-EGFP-WPRE-hGH poly(A) (Alex_02)
Plasmid#71651PurposeAAV expression of EGFP in human and mouse neuronsDepositorInsertEGFP
UseAAVExpressionMammalianAvailable SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBluescript II Ks(+)-ZsGreen1-SV40 PolyA-Stop cKit HDR
Plasmid#207135PurposeDNA sequence source for amplifying an HDR template for mouse cKit knockout and reporter knock-inDepositorInsert5'HDR cKit- ZsGreen- sv40(polyA) - 3'HDR cKit (Kit Mouse)
ExpressionMammalianAvailable SinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBluescript II Ks(+)-ZsGreen1-SV40 PolyA-Stop Mettl3 HDR
Plasmid#207136PurposeDNA sequence source for amplifying an HDR template for mouse Mettl3 knockout and reporter knock-inDepositorInsert5'HDR Mettl3 - ZsGreen- sv40(polyA) - 3'HDR Mettl3 (Mettl3 Mouse)
ExpressionMammalianAvailable SinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Recruited polymerase expression - pCMV-T7-Phi29(+exo)-CC(N5) (LM2761)
Plasmid#208970PurposeRecruited Phi29(+exo) DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.DepositorInsertPhi29(+exo)-BPNLS-CC(N5)
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianPromoterCMV and T7Available SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-EGFPnls barcode-210-SV40 polyA
Plasmid#190876PurposeFor barcoded retrograde labelingDepositorInsertEGFPnls-barcode210
UseAAVMutationWTAvailable SinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1C-no5UTR-Fluc-MtoL-V5-5BoxB-polyA-HhR_AC
Plasmid#148409PurposeInsect Expression of 5BoxB-polyA-HhRDepositorInsert5BoxB-polyA-HhR
ExpressionInsectAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-CrPV-Rluc-6xbsMS2-PolyA-Malat_AC
Plasmid#148446PurposeMammalian Expression of 6xbsMS2-PolyA-MalatDepositorInsert6xbsMS2-PolyA-Malat
ExpressionMammalianAvailable SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HCV-RLuc-6xbsMS2-polyA-Malat_AC
Plasmid#148448PurposeMammalian Expression of 6xbsMS2-polyA-MalatDepositorInsert6xbsMS2-polyA-Malat
ExpressionMammalianAvailable SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19-TgKI-MCS-p2A-QF2-MCS-polyA
Plasmid#174026PurposeThis plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.DepositorInsertp2A-QF2
UseZebrafish knock-in taggingAvailable SinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only