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We narrowed to 693 results for: emd

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Showing: 521 - 560 of 693 results

  2. pCJ217

    Plasmid
    #162685
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating two point mutations, G489C and S530C, to introduce a 6th disulfide bond (from PETase).
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), with mutations to introduce a 6th disulfide bond from PETase
    Tags
    His
    Expression
    Bacterial
    Mutation
    G489C, S530C; codon optimized for E. coli K12
    Promoter
    T7/lac
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    162685-plasmid-map-sequence-id-320886

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  3. pCJ208

    Plasmid
    #162681
    Purpose
    Lidded variant of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) removing a seven-residue loop of PETase (Trp185:Phe191, PETase numbering) and replacing it with Gly251:Thr472 from MHETase; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) incorporating the MHETase lid
    Tags
    His
    Expression
    Bacterial
    Mutation
    Replacement of W185-F191 with G251-T472 from MHET…
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162681-plasmid-map-sequence-id-321444

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  4. pCJ209

    Plasmid
    #162682
    Purpose
    Lidless variant of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) replacing Gly251:Thr472 of MHETase with the 7-residue loop (Trp185:Phe191, PETase numbering) of PETase , codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) with the lid removed
    Tags
    His
    Expression
    Bacterial
    Mutation
    Replacement of G251-T472 with W185-F191 from PETa…
    Promoter
    T7/lac
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    162682-plasmid-map-sequence-id-320836

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  5. pCJ200

    Plasmid
    #162673
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S136C and a sequence Tyr75:Phe104 to introduce a 6th disulfide bond as in AoFaeB-2.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    S136C and a sequence Y75-F104 to introduce a 6th…
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162673-plasmid-map-sequence-id-321521

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  6. pCJ210

    Plasmid
    #162683
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S136C, G489C, S530C, and a sequence Tyr75:Phe104 to introduce a 6th and 7th disulfide bond as in AoFaeB-2.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    S136C, G489C, S530C, and a sequence Y75-F104 to i…
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162683-plasmid-map-sequence-id-321486

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  7. pCJ220

    Plasmid
    #162686
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating lid deletion (Gly251:Thr472) and C224W and C529S mutations from PETase active site.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) with lid deletion and C224W and C529S mutations
    Tags
    His
    Expression
    Bacterial
    Mutation
    deltaG251-T472, C224W, C529S; codon optimized for…
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162686-plasmid-map-sequence-id-321468

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  8. pCJ221

    Plasmid
    #162687
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating lid deletion (Gly251:Thr472) and C224H and C529F mutations from PETase active site.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), with lid deletion and C224H and C529F mutations
    Tags
    His
    Expression
    Bacterial
    Mutation
    deltaG251-T472, C224H, C529F; codon optimized for…
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162687-plasmid-map-sequence-id-321457

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  9. pOPINH

    Plasmid
    #53537
    Purpose
    pTriex-based vector with signal sequence and N-his tag for protein expression
    Depositor
    Ray Owens
    Type
    Empty backbone
    Expression
    Bacterial, Insect, and Mamm…
    Available Since
    June 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    53537-plasmid-map-sequence-id-83418

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  10. pOPINEneo

    Plasmid
    #53536
    Purpose
    pTriex-based expression vector which adds a C-his8 tag to the inserted gene
    Depositor
    Ray Owens
    Type
    Empty backbone
    Expression
    Bacterial, Insect, and Mamm…
    Available Since
    June 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    53536-plasmid-map-sequence-id-83415

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  11. pOPINN-GFP

    Plasmid
    #53541
    Purpose
    pTirex based GFP fusion vector for expression in mammalian, E. coli and insect cells using the baculovirus system
    Depositor
    Ray Owens
    Insert
    GFP
    Tags
    N-His-GFP
    Expression
    Bacterial, Insect, and Mamm…
    Available Since
    June 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    53541-plasmid-map-sequence-id-83419

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  13. pOPINEneo-3C-GFP

    Plasmid
    #53534
    Purpose
    pTirex based GFP fusion vector for expression in mammalian, E. coli and insect cells using the baculovirus system
    Depositor
    Ray Owens
    Insert
    GFP
    Tags
    GFP-His8
    Expression
    Bacterial, Insect, and Mamm…
    Available Since
    June 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    53534-plasmid-map-sequence-id-83411

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  15. LaminA-C-18

    Plasmid
    #58193
    Purpose
    Localization: Nuclear Envelope, Excitation: N/A, Emission: N/A
    Depositor
    Michael Davidson
    Insert
    LaminA (LMNA Human)
    Tags
    SNAP tag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  16. Lamin A-mRFP

    Plasmid
    #124268
    Purpose
    Wild-type Lamin A, C-terminally tagged with mRFP
    Depositor
    Eric Schirmer
    Insert
    LMNA (LMNA Human)
    Tags
    mRFP
    Expression
    Mammalian
    Available Since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124268-plasmid-map-sequence-id-241940

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  17. mEmerald-LaminA-C-18

    Plasmid
    #54138
    Purpose
    Localization: Nuclear Envelope, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    LaminA (LMNA Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 10, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54138-plasmid-map-sequence-id-271362

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  18. mEmerald-LaminA-N-18

    Plasmid
    #54139
    Purpose
    Localization: Nuclear Envelope, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    LaminA (LMNA Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 10, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. 3BFN

    Plasmid
    #60111
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    KIF22:KIF22_dml-X01:C200692 (KIF22 Human)
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available Since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  20. mCherry-LaminA-C-18

    Plasmid
    #55068
    Purpose
    Localization: Nuclear Envelope, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Lamin A (LMNA Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55068-plasmid-map-sequence-id-200503

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  21. Lamin A (R377H)-mRFP

    Plasmid
    #124270
    Purpose
    Lamin A mutant (R377H), C-terminally tagged with mRFP. Mutation associated with EDMD myopathy.
    Depositor
    Eric Schirmer
    Insert
    LMNA (R377H) (LMNA Human)
    Tags
    mRFP
    Expression
    Mammalian
    Mutation
    LMNA (R377H)
    Available Since
    July 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    124270-plasmid-map-sequence-id-251113

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  22. Lamin A (R435C)-mRFP

    Plasmid
    #124274
    Purpose
    Lamin A mutant (R435C), C-terminally tagged with mRFP. Mutation associated with Progeria.
    Depositor
    Eric Schirmer
    Insert
    LMNA (R435C) (LMNA Human)
    Tags
    mRFP
    Expression
    Mammalian
    Mutation
    LMNA (R435C)
    Available Since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124274-plasmid-map-sequence-id-241955

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  23. Lamin A (G465D)-mRFP

    Plasmid
    #124271
    Purpose
    Lamin A mutant (G465D), C-terminally tagged with mRFP. Mutation associated with FPLD lipodystrophy.
    Depositor
    Eric Schirmer
    Insert
    LMNA (G465D) (LMNA Human)
    Tags
    mRFP
    Expression
    Mammalian
    Mutation
    LMNA (G465D)
    Available Since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124271-plasmid-map-sequence-id-241962

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  24. PA-mCherry1-LaminA-C-18

    Plasmid
    #57185
    Purpose
    Localization: Nuclear Envelope, Excitation: 400 / 504, Emission: 515 / 517
    Depositor
    Michael Davidson
    Insert
    LaminA (LMNA Human)
    Tags
    PA-mCherry1
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 13, 2015
    Availability
    Academic Institutions and Nonprofits only

  25. Lamin A (R527P)-mRFP

    Plasmid
    #124272
    Purpose
    Lamin A mutant (R527P), C-terminally tagged with mRFP. Mutation associated with EDMD myopathy.
    Depositor
    Eric Schirmer
    Insert
    LMNA (R527P) (LMNA Human)
    Tags
    mRFP
    Expression
    Mammalian
    Mutation
    LMNA (R527P)
    Available Since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124272-plasmid-map-sequence-id-241977

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  26. mEos3.2-LaminA-C-18

    Plasmid
    #57463
    Purpose
    Localization: Nuclear Envelope, Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    LaminA (LMNA Human)
    Tags
    mEos3.2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 5, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  27. Lamin A (R482Q)-mRFP

    Plasmid
    #124273
    Purpose
    Lamin A mutant (R482Q), C-terminally tagged with mRFP. Mutation associated with FPLD lipodystrophy.
    Depositor
    Eric Schirmer
    Insert
    LMNA (R482Q) (LMNA Human)
    Tags
    mRFP
    Expression
    Mammalian
    Mutation
    LMNA (R482Q)
    Available Since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124273-plasmid-map-sequence-id-241964

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  28. Lamin A (R298C)-mRFP

    Plasmid
    #124269
    Purpose
    Lamin A mutant (R298C), C-terminally tagged with Mrfp. Mutation associated with CMT neuropathy.
    Depositor
    Eric Schirmer
    Insert
    LMNA (R298C) (LMNA Human)
    Tags
    mRFP
    Expression
    Mammalian
    Mutation
    LMNA (R298C)
    Available Since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124269-plasmid-map-sequence-id-241969

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  29. mEos4b-LaminA-C-18

    Plasmid
    #57511
    Purpose
    Localization: Nuclear Envelope, Excitation: 505 / 569, Emission: 516 / 581
    Depositor
    Michael Davidson, Loren Looger
    Insert
    LaminA (LMNA Human)
    Tags
    mEos4b
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 4, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  30. mEos4a-LaminA-C-18

    Plasmid
    #57497
    Purpose
    Localization: Nuclear Envelope, Excitation: 505 / 569, Emission: 516 / 581
    Depositor
    Michael Davidson, Loren Looger
    Insert
    LaminA (LMNA Human)
    Tags
    mEos4a
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 4, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  31. mEos2-LaminA-C-18

    Plasmid
    #57394
    Purpose
    Localization: Nuclear Envelope, Excitation: 505 / 569, Emission: 516 / 581
    Depositor
    Michael Davidson
    Insert
    LaminA (LMNA Human)
    Tags
    mEos2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 5, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  34. pAAV-AcGFP-progerin

    Plasmid
    #86947
    Purpose
    Construct used to synthesize progerin mRNA. Please note the plasmid contains LMNA gene with nucleotide C1824T mutation.
    Depositor
    Alex Hewitt
    Insert
    AcGFP-progerin (LMNA Human)
    Use
    AAV
    Tags
    AcGFP
    Expression
    Mammalian
    Mutation
    progerin sequence is LMNA gene with nucleotide C1…
    Promoter
    CMV and T7
    Available Since
    June 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    86947-plasmid-map-sequence-id-180625

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Showing: 521 - 560 of 693 results