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Plasmid#86657PurposeLuciferase reporter containing CDK4 promoterDepositorInsertCDK4 promoter (CDK4 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutation867 base pairs including up to coding nucleotide …PromoterCDK5Available sinceJune 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
beta4-nAChR shRNA (Beta-4.3)
Plasmid#86650Purposeexpression of shRNA targeting CHRNB4DepositorInsertCHRNB4 (beta4-nAChR) (CHRNB4 Human)
UseRNAiTagsExpressionMammalianMutationPromoterH1Available sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
CDk4-luciferase fragment A
Plasmid#86658PurposeLuciferase reporter containing CDK4 promoterDepositorInsertCDK4 promoter (CDK4 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationgenerated by cleavage at the NcoI site from 880 (…PromoterCDK6Available sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRDA_052_HygroR
Plasmid#228937PurposePlasmid for cloning of custom gRNA using EnAsCas12aDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
GvpA-CaM
Plasmid#223555PurposeFull length gas vesicle coat protein A derived from H. salinarum (GvpA) fused to calmodulin.DepositorInsertpET300-GvpACaM
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pM-mRuby2
Plasmid#176550PurposeExpression of mRuby2 for auxotrophic selection in the absence of methionineDepositorInsertyomRuby2
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pL-mWasabi
Plasmid#176552PurposeExpression of mWasabi for auxotrophic selection in the absence of leucineDepositorInsertmWasabi
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pM-eCFP
Plasmid#176558PurposeExpression of eCFP for auxotrophic selection in the absence of methionineDepositorInserteCFP
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pH-TagRFP657
Plasmid#176560PurposeExpression of TagRFP657 for auxotrophic selection in the absence of lysineDepositorInsertTagRFP657
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pM-TagRFP657
Plasmid#176563PurposeExpression of TagRFP657 for auxotrophic selection in the absence of methionineDepositorInsertTagRFP657
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Flag-stm2585_L139*
Plasmid#174400PurposeS. Typhimurium gene stm2585 (sarA/steE) truncated after 139th amino acid, which removes the stretch homologous to gp130DepositorInsertsarA
UseTagsEGFP and FlagExpressionMammalianMutationL139* (Truncation after 139th amino acid in sarA)PromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Flag-stm2585_S130A
Plasmid#174399PurposeNative S. Typhimurium gene stm2585 (sarA/steE) with N-terminal GFP and Flag tags and its GSK3-binding SxxxSP motif mutated to SxxxAPDepositorInsertsarA
UseTagsEGFP and FlagExpressionMammalianMutationS130APromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Flag-stm2585_Y167F
Plasmid#174398PurposeNative S. Typhimurium gene stm2585 (sarA/steE) with N-terminal GFP and Flag tags and its STAT3-binding YxxQ motif mutated to FxxQDepositorInsertsarA
UseTagsEGFP and FlagExpressionMammalianMutationY167FPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag-stm2585-gp130
Plasmid#174393PurposeChimera of codon-optimized stm2585 (sarA/steE) and human gp130 sequence.DepositorInsertsarA-IL6ST chimera
UseTagsFlagExpressionMammalianMutation140-177 of sarA replaced with homologous IL6ST se…PromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag-stm2585_Y167F
Plasmid#174392PurposeS. Typhimurium gene stm2585 (sarA/steE) codon-optimized for mammalian expression with STAT3-binding YxxQ motif mutated to FxxQDepositorInsertstm2585
UseTagsFlagExpressionMammalianMutationTyr 167 changed to PhePromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
CDk4-luciferase fragment B
Plasmid#86659PurposeLuciferase reporter containing CDK4 promoterDepositorInsertCDK4 promoter (CDK4 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationgenerated by cleavage at the KpnI site from 880 (…PromoterCDK7Available sinceJune 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXPR_051_dCas9-KRAB-HA
Plasmid#228936PurposeAll-in-one dCas9-KRAB-MeCP2 plasmid for cloning of custom sgRNADepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgRNA-Cre-GpNLuc
Plasmid#208382PurposeDerived from LentiCRISPRV2 by replacing Cas9 with Cre recombinase and puromycin resistance with GpNLuc. Contains a stuffer sequence for cloning of sgRNA of interest, as per LentiCRISPRV2.DepositorTypeEmpty backboneUseLentiviral and LuciferaseTagsExpressionMammalianMutationWTPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRDA355_sgCiPOLR2D
Plasmid#229022PurposeExpression of a CRISPRi doxycycline inducible guide targeting POLR2DDepositorInsertPOLR2D gRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRDA355_sgCiPELO #2
Plasmid#229020PurposeExpression of a CRISPRi doxycycline inducible guide targeting PELODepositorInsertPELO gRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRDA052H_sgCh2
Plasmid#229023PurposeExpression of a EnAsCas12a control guide that cuts an intergenic region on chromosome 2DepositorInsertsgChr2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRDA_174_neoR
Plasmid#228935PurposeLentiviral expression of EnAsCas12aDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRDA052H_sgFOCAD #2
Plasmid#229024PurposeExpression of a EnAsCas12a guide targeting FOCADDepositorInsertFOCAD gRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXPR_051d_sgCiChr2-2
Plasmid#228940PurposeExpression of a CRISPRi control sgRNA that cuts an intergenic region on chromosome 2DepositorInsertsgChr2-2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-mCHERRY
Plasmid#224298PurposeExpresses mCherry under EF1a promoter (CTRL vector)DepositorInsertmCherry
UseRetroviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
MFE23-ZZ
Plasmid#223553PurposeMFE23-ZZ, a bispecific adaptor molecule, was created by fusing a scFv, termed MFE23, recognizing carcinoembryonic antigen to protein ZZ, which binds to the Fc fragment of IgG.DepositorInsertpET302-MFE23-ZZ
UseTagsHA tag and HIS tagExpressionBacterialMutationPromoterT7Available sinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFastBacHTA-NEDD4L_FL
Plasmid#187722PurposeExpress His-tagged NEDD4L FL in insect cellsDepositorInsertNEDD4L FL (NEDD4L Human)
UseTagsHis-TEVExpressionInsectMutationPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
His-Precission-NEDD4L_FL_pCDNA3.1(+)
Plasmid#187730PurposeExpress NEDD4L FL in mammalian cellsDepositorInsertNEDD4L FL (NEDD4L Human)
UseTagsHis-HRV 3C siteExpressionMammalianMutationPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-NEDD4L_FL
Plasmid#187712PurposeExpress GST-tagged NEDD4L FL in bacterial cellsDepositorInsertNEDD4L FL (NEDD4L Human)
UseTagsGST-HRV 3C siteExpressionBacterialMutationPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-NEDD4L_FL
Plasmid#187705PurposeExpress his-tagged NEDD4L FL in bacterial cellsDepositorInsertNEDD4L FL (NEDD4L Human)
UseTagsHis-HRV 3C siteExpressionBacterialMutationPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
ATG9A-mCherry
Plasmid#197393PurposeExpresses ATG9A in mammalian cellsDepositorInsertATG9A (ATG9A Human)
UseTagsmChExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGK-HELLO-T2A-Cre LV
Plasmid#202003PurposeGenerates lentiviral vector for autochthonous KP-HELLO tumor initiation by intratracheal administration to KP miceDepositorInsertHELLO-T2A-Cre
UseLentiviralTagsFLAGExpressionMutationPromoterpGKAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro-Halo-hMRE11(wt)
Plasmid#208393PurposeTo generate stable cell line expressing Halo-human MRE11(WT) by Retroviral infection.DepositorInsertHuman MRE11 (MRE11 Human)
UseLuciferase and RetroviralTagsExpressionMammalianMutationH129NPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro-Halo-hMRE11(H129N)
Plasmid#208394PurposeTo generate stable cell line expressing Halo-human MRE11(H129N) by Retroviral infection.DepositorInsertHuman MRE11 (MRE11 Human)
UseLuciferase and RetroviralTagsExpressionMammalianMutationH129NPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro U6 sgRNA BfuAI stuffer A-tract
Plasmid#138525PurposeExpresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt control A-tract repeat RNA sequence.DepositorInsertControl non-PRC2 binding A-tract repeat RNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro U6 sgRNA BfuAI stuffer G-tract
Plasmid#138524PurposeExpresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt PRC2-binding G-tract repeat RNA sequence.DepositorInsertPRC2-binding G-tract repeat RNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXPR_023d sgCiPELO #1
Plasmid#228932PurposeExpression of dCas9-KRAB and sgRNA targeting PELODepositorInsertPELO gRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRDA355_sgCiCh2-2
Plasmid#229021PurposeExpression of a CRISPRi doxycycline inducible control sgRNA that cuts an intergenic region on chromosome 2DepositorInsertsgChr2-2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXPR_023d sgCiPELO #8
Plasmid#228934PurposeExpression of dCas9-KRAB and sgRNA targeting PELODepositorInsertPELO gRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-NEDD4L_ΔC2 (157)
Plasmid#187720PurposeExpress GST-tagged NEDD4L ΔC2 (157) in insect cellsDepositorInsertNEDD4L residues 157-975 (NEDD4L Human)
UseTagsGST-HRV 3C siteExpressionInsectMutationdeletion of residues 1-156PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-NEDD4L_W2-W3-W4-HECT (349)
Plasmid#187721PurposeExpress GST-tagged NEDD4L W2-W3-W4-HECT (349) in insect cellsDepositorInsertNEDD4L residues 349-975 (NEDD4L Human)
UseTagsGST-HRV 3C siteExpressionInsectMutationdeletion of residues 1-348PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBacHTA-NEDD4L_C2-W1-L-W2-HECT
Plasmid#187723PurposeExpress His-tagged NEDD4L C2-W1-L-W2-HECT chimera in insect cellsDepositorInsertNEDD4L residues 1-418+582-975 (NEDD4L Human)
UseTagsHis-TEVExpressionInsectMutationdeletion of residues 419-581PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
His-Precission-NEDD4L_C942S_pCDNA3.1(+)
Plasmid#187729PurposeExpress the catalytically inactive NEDD4L FL in mammalian cellsDepositorInsertNEDD4L FL with C874 mutated to a serine (NEDD4L Human)
UseTagsHis-HRV 3C siteExpressionMammalianMutationCys874 mutated to a serinePromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-NEDD4L_W3-W4-HECT (492)
Plasmid#187709PurposeExpress his-tagged NEDD4L W3-W4-HECT in bacterial cellsDepositorInsertNEDD4L residues 492-975 (NEDD4L Human)
UseTagsHis-HRV 3C siteExpressionBacterialMutationdeletion of residues 1-491PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-NEDD4L_W4-HECT
Plasmid#187710PurposeExpress his-tagged NEDD4L W4-HECT in bacterial cellsDepositorInsertNEDD4L residues 543-975 (NEDD4L Human)
UseTagsHis-HRV 3C siteExpressionBacterialMutationdeletion of residues 1-542PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-NEDD4L_FL-CS
Plasmid#187713PurposeExpress the catalytically inactive GST-tagged NEDD4L FL in bacterial cellsDepositorInsertNEDD4L FL with C874 mutated to a serine (NEDD4L Human)
UseTagsGST-HRV 3C siteExpressionBacterialMutationCys874 mutated to a serinePromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-NEDD4L_W2-W3-W4-HECT (348)
Plasmid#187716PurposeExpress GST-tagged NEDD4L W2-W3-W4-HECT (348) in bacterial cellsDepositorInsertNEDD4L with residues XX (NEDD4L Human)
UseTagsGST-HRV 3C siteExpressionBacterialMutationdeletion of residues 1-347PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-NEDD4L_WW34
Plasmid#187718PurposeExpress GST-tagged NEDD4L WW34 tandem domains in bacterial cellsDepositorInsertNEDD4L residues 492-581 (NEDD4L Human)
UseTagsGST-HRV 3C siteExpressionBacterialMutationonly residues 492-581PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-NEDD4L_HECT
Plasmid#187719PurposeExpress GST-tagged NEDD4L HECT domain in bacterial cellsDepositorInsertNEDD4L residues 594-975 (NEDD4L Human)
UseTagsGST-HRV 3C siteExpressionBacterialMutationdeletion of residues 1-593PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-NEDD4L_W2-W3-W4-HECT (381)
Plasmid#187708PurposeExpress his-tagged NEDD4L W2-W3-W4-HECT in bacterial cellsDepositorInsertNEDD4L residues 381-975 (NEDD4L Human)
UseTagsHis-HRV 3C siteExpressionBacterialMutationdeletion of residues 1-380PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only