We narrowed to 5,492 results for: 129

1. 3xnls-cpmTq2-Calcium-lifetime-sensor

   Plasmid

   #129626

   Purpose

   Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes

   Depositor

   Dorus Gadella

   Article

   van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

   Insert

   3xnls-cpmTq2-Calcium-lifetime-sensor
   

   Use

   Tags

   3xNLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAH-CTX1-rhadCas9-native

   Plasmid

   #129392

   Purpose

   Derived from pAH-CTX1-rha. The native Streptococcus pyogenes dCas9 gene cloned downstream of PrhaBAD. Non-codon-optimized dCas9 version of pAH-CTX1-rhadCas9.

   Depositor

   Silvia Cardona

   Article

   Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

   Insert

   S. pyogenes dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Aspartate 10 to Alanine, Histidine 840 to Alanine

   Promoter

   PrhaBAD

   Available since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. mCherry-PLEKHG4

   Plasmid

   #129618

   Purpose

   RhoGEF

   Depositor

   Dorus Gadella

   Article

   Reinhard et al Small GTPases. 2019 Aug 30:1-15. doi: 10

   Insert

   PLEKHG4
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV 3xFLAG-ATG3 C264A

   Plasmid

   #129297

   Purpose

   Expresses 3xFLAG-ATG3 C264A in mammalian cells

   Depositor

   Robin Ketteler

   Article

   Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

   Insert

   Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   changed Cysteine 264 to Alanine

   Promoter

   CMV

   Available since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFLIPglu-2uDelta13

   Plasmid

   #12990

   Depositor

   Wolf Frommer

   Article

   Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

   Insert

   FLIPglu F16F D152A Delta13
   

   Use

   Plant nanosensor

   Tags

   ECFP and EYFP

   Expression

   Plant

   Mutation

   D152A mutation in binding protein

   Promoter

   Available since

   Jan. 5, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pYPQ292

   Plasmid

   #129672

   Purpose

   Gateway entry clone for CRISPR-Cas12b systems

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   AaCas12b
   

   Use

   CRISPR; Gateway compatible aacas12b entry clone

   Tags

   Expression

   Plant

   Mutation

   AaCas12b is rice codon optimized

   Promoter

   Available since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUC57-PB-PGK-tetR-KRAB-2A-Puro

   Plasmid

   #129536

   Purpose

   PiggyBac transposon carrying tetR-KRAB-2A-Puro under the PGK promoter.

   Depositor

   Hideyuki Okano

   Article

   Tsujimura et al Elife. 2020 May 5;9. pii: 47980. doi: 10

   Insert

   PB-PGK-tetR-KRAB-2A-Puro
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   hPGK promoter

   Available since

   Dec. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCAG-Ace2_D92N_E199V-mRuby3-ST

   Plasmid

   #129262

   Purpose

   Express soma-localized Ace2_D92N_E199V-mRuby3 in mammalian cells

   Depositor

   Eric Schreiter

   Article

   Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

   Insert

   Ace2_D92N_E199V-mRuby3-ST
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D92N, E199V

   Promoter

   CAG

   Available since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pB-RTP2-Integration

   Plasmid

   #129459

   Purpose

   piggyBac transposon vector that Inducibly (Tet-On) expresses RTP2

   Depositor

   Sriram Kosuri

   Article

   Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

   Insert

   RTP2 (RTP2 Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE (Tet-On)

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-CAG-DIO-(STChRger2-TS-EYFP)

    Plasmid

    #129394

    Purpose

    Soma Targeted, High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Uses the CAG promoter and double floxed.

    Depositor

    Viviana Gradinaru

    Article

    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

    Insert

    soma targeted ChRger2
    

    Use

    AAV and Cre/Lox

    Tags

    Kv2.1-TS-EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pALS297 (ttTi5605-MCS loxP::Prps-27::NeoR::unc-54-3ʹutr::loxP)

    Plasmid

    #129538

    Purpose

    modified pCFJ151 for C. elegans mosSCI at ttTi5605 or oxTi354 in otherwise wild-type background; floxed NeoR selection

    Depositor

    Arneet Saltzman

    Article

    Aram et al MicroPubl Biol. 2019 Aug 27;2019. doi: 1

    Type

    Empty backbone

    Use

    Cre/Lox; Mos1-mediated single copy insertion (mos…

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLBS.CAG/NLS-mScarlet

    Plasmid

    #129336

    Purpose

    nucleus marker, lentivirus

    Depositor

    Yasushi Okada

    Insert

    mScarlet (Addgene 85042)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

13. pGEX-4T1 NLS-GFP

    Plasmid

    #129441

    Purpose

    bacterial expression of GFP

    Depositor

    Oskar Laur

    Insert

    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pgRNA-non-target

    Plasmid

    #129465

    Purpose

    Derived from pSCB2-sgRNA. 20 nucleotide sequence added as sgRNA binding region by inverse PCR. Used as a negative control.

    Depositor

    Silvia Cardona

    Article

    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

    Insert

    Non-target/random gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119 (SpeI)

    Available since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFLIPglu-600uDelta13

    Plasmid

    #12991

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

    Insert

    FLIPglu F16A Delta13
    

    Use

    Plant nanosensor

    Tags

    ECFP and EYFP

    Expression

    Plant

    Mutation

    F16A in binding protein

    Promoter

    Available since

    Jan. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pGEX-4T-1-3xMyc

    Plasmid

    #129569

    Purpose

    Empty backbone for Myc Tag

    Depositor

    Kevin Janes

    Article

    Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pN1-OPA1(123Nt)-mNG

    Plasmid

    #129344

    Purpose

    Mitochondrial intermembrane space localization

    Depositor

    Yasushi Okada

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pN1-OPA1(123Nt)-Halo

    Plasmid

    #129345

    Purpose

    Halo Tag, Mitochondrial intermembrane space localization

    Depositor

    Yasushi Okada

    Insert

    Halo (Promega)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMM0641

    Plasmid

    #129006

    Purpose

    Integration vector for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mCherry-PLEKHG2

    Plasmid

    #129617

    Purpose

    RhoGEF

    Depositor

    Dorus Gadella

    Article

    Reinhard et al Small GTPases. 2019 Aug 30:1-15. doi: 10

    Insert

    PLEKHG2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge