We narrowed to 350 results for: dr-gfp
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Plasmid#172850PurposeDRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B9 (Zhong et al, eLife 2021), mEGFP translational phase (2-2), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).DepositorInsertDRS-2 sgRNA and CRISPIE designer exon (phase 2-2) encoding mEGFP
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330 _CRISPIE donor B10
Plasmid#172851PurposeDRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B10 (Zhong et al, eLife 2021), mEGFP translational phase (2-stop), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).DepositorInsertDRS-2 sgRNA and CRISPIE designer exon (phase 2-stop) encoding mEGFP
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMK191
Plasmid#226658PurposeBroad host range WVO1 vector that replicates in Synechococcus sp. PCC 7002, GentR, contains HIP1 siteDepositorTypeEmpty backboneUseUnspecifiedAvailable SinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMK74
Plasmid#226657PurposeBroad host range WVO1 vector that replicates in Synechococcus sp. PCC 7002, KanR, contains HIP1 siteDepositorTypeEmpty backboneUseUnspecifiedAvailable SinceJan. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV_ACTB CRISPIE
Plasmid#172853PurposeActb sgRNA & DRS-2 sgRNA expression under U6. CRISPIE donor mEGFP phase (0-0), excised by DRS-1 or DRS-2 (with SpCas9). Also expresses mRuby3 under the CBA promotor. See Zhong et al, eLife 2021.DepositorInsertsActb intron 1 sgRNA
DRS-2 sgRNA
CRISPIE designer exon (phase 0-0) encoding mEGFP
mRuby3
UseAAV and CRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDA313
Plasmid#195214PurposeExpression of superfolder GFP (sfGFP) by a hybrid T7 promoter with tetO.DepositorInsertsfGFP with tetO and a hairpin
ExpressionBacterialPromoterT7Available SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRi
Plasmid#199808Purposevector for constitutive CRISPRi-mediated gene knockdown in S. aureusDepositorInsertsdCas9
sgRNA with GFP cassette
UseCRISPRPromoterP23 and PRAB17 (sgRNA) and SarAP1 (GFP)Available SinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCLucf
Plasmid#37328DepositorAvailable SinceJuly 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pX330 _HITI donor B6
Plasmid#172847PurposeDRS-2 sgRNA expression under a U6 promotor and HITI donor B6 (Zhong et al, eLife 2021), mEGFP translational phase (1-1), excised by DRS-2 sgRNA (with SpCas9).DepositorInsertDRS-2 sgRNA and HITI donor (phase 1) encoding mEGFP
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJMK004
Plasmid#34616PurposeViral expression of Arch (D95N mutant with greater sensitivity but slower response) voltage indicatorDepositorInsertArch D95N
UseLentiviralTagsGFPExpressionMammalianMutationChanged Aspartic Acid 95 to AsparaginePromoterCaMKIIAvailable SinceJan. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pWT004a
Plasmid#107883PurposeR1 plasmid. Contains PBAD-EGFP and is part of the CAMERA system for rewritable multi-event analog recording in bacteriaDepositorInsertPBAD-EGFP
ExpressionBacterialAvailable SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWT018a
Plasmid#107885PurposeR3 plasmid. Contains PBAD-EGFP and is part of the CAMERA system for rewritable multi-event analog recording in bacteriaDepositorInsertPBAD-EGFP
ExpressionBacterialAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWT009d
Plasmid#107888PurposeR6 plasmid. Contains PBAD-EGFP and is part of part of the CAMERA system for rewritable multi-event analog recording in bacteriaDepositorInsertPBAD-EGFP
ExpressionBacterialAvailable SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWT018e
Plasmid#107886PurposeR4 plasmid. Contains PBAD-EGFP-151TGA and is part of the CAMERA system for rewritable multi-event analog recording in bacteriaDepositorInsertPBAD-EGFP-151TGA
ExpressionBacterialAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWT004b
Plasmid#107884PurposeR2 plasmid. Contains PBAD-EGFP-151TGA and is part of the CAMERA system for rewritable multi-event analog recording in bacteriaDepositorInsertPBAD-EGFP-151TGA
ExpressionBacterialAvailable SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lenti-PEAR-mCherry
Plasmid#228892PurposeAll-in-one lentiviral version of the PEAR reporter. Constitutive RFP expression with GFP turned on in event of prime editing.DepositorInsertEGFP-IRES-mScarlet
UseCRISPR and LentiviralExpressionMammalianAvailable SinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
SHC003BSD
Plasmid#133300PurposeThe puromycin selection marker was replaced by blasticidin in SHC003. This construct could be used together with other plasmid contains puromycin selection marker.DepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable SinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWT004j
Plasmid#107887PurposeR5 plasmid. Contains PBAD-EGFP-115-135insertion, T206G and is part of the CAMERA system for rewritable multi-event analog recording in bacteriaDepositorInsertPBAD-EGFP-115-135insertion, T206G
ExpressionBacterialMutationinsertion of nt 115-135, T206G nt changeAvailable SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDT305
Plasmid#128801PurposeHuman codon optimized dCas9-Dnmt3a-Dnmt3l-T2A-eGFP + gRNA expression cassette + LV backboneDepositorInsertdCas9-Dnmt3a-Dnmt3l-T2A-eGFP + gRNA expression cassette + LV backbone
UseCRISPR and LentiviralPromoterhUBC promoterAvailable SinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLAREG
Plasmid#73044PurposeInducible expression of EGFP in yeast. Has dual-mode promoter with activation and repression capability for tuning gene expression in yeast.DepositorInsertsEGFP
LacI
ExpressionYeastPromoterGAL1 promoter and hybrid dual-mode promoter-see c…Available SinceFeb. 4, 2016AvailabilityAcademic Institutions and Nonprofits only