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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 21,779 results for: his

Showing: 261 - 280 of 21779 results

  2. pGS140_6xHis-EGFP-I3-01

    Plasmid
    #211889
    Purpose
    Bacterial expression of I3-01
    Depositor
    Iain Cheeseman
    Insert
    I3-01
    Use
    Tags
    Expression
    Bacterial
    Mutation
    N/A
    Promoter
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    211889-plasmid-map-sequence-id-426370

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  5. pFastBac1-His10FLAG

    Plasmid
    #157736
    Purpose
    empty pFastBac1 vector (polyhedrin promoter) adds enterokinase-removable N-terminal His10-tag + 3xFLAG tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites
    Depositor
    Debra Hansen
    Article
    Olson et al Protein Expr Purif. 2021 May 7:105890. d
    Type
    Empty backbone
    Use
    Tags
    His10 + 3xFLAG, enterokinase-cleavable
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    157736-plasmid-map-sequence-id-333926

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  6. pGEX6P1-N-His

    Plasmid
    #119755
    Purpose
    Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal 10x His tag.
    Depositor
    Martin Reijns, Andrew Jackson
    Type
    Empty backbone
    Use
    Tags
    10xHis and GST
    Expression
    Bacterial
    Mutation
    Promoter
    tac promoter
    Available since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    119755-plasmid-map-sequence-id-233662

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  8. pMC059-HisMS2_PLP_NucPhos_pac

    Plasmid
    #155039
    Purpose
    Generation of MS2 Virus Like Particles packaged with the sequence for the SARS-CoV-2 Nucleocapsid Phosphoprotein gene
    Depositor
    Paul Freemont
    Article
    Crone et al Nat Commun. 2020 Sep 8;11(1):4464. doi:
    Inserts
    Maturation Protein
    Coat Protein Dimer
    Nucleocapsid Phosphoprotein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Remove TypeIIs Restriction Sites
    Promoter
    T7
    Available since
    Sept. 9, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    155039-plasmid-map-sequence-id-308137

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  10. 6HisVDAC1/pCDNA3

    Plasmid
    #197320
    Purpose
    Plasmid expressing an antigen targeting the VDAC1 transporter
    Depositor
    Dan Minor
    Insert
    VDAC1 (VDAC1 Human)
    Use
    Tags
    His
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    197320-plasmid-map-sequence-id-388161

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  13. pTD-NStrepHis

    Plasmid
    #45936
    Purpose
    use to create N-terminal Strep-tagII fusion proteins or dual tagged fusion with N-terminal Strep-tagII and C-terminal 6x His-tag
    Depositor
    Thorben Dammeyer
    Article
    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.
    Type
    Empty backbone
    Use
    Tags
    6xHis and Strep II
    Expression
    Bacterial
    Mutation
    Promoter
    Trc (lactose/IPTG inducible)
    Available since
    Sept. 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    45936-plasmid-map-sequence-id-64475

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  14. pET_RTX-6xHis

    Plasmid
    #145029
    Purpose
    pET vector for IPTG-inducible, T7-based expression of RTX bearing a C-terminal 6xHis tag
    Depositor
    Andrew Ellington
    Article
    Bhadra et al BioProtocol 2021
    Insert
    RTX
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    145029-plasmid-map-sequence-id-292639

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  18. pFastBac1-His10TEV

    Plasmid
    #159427
    Purpose
    empty pFastBac1 vector (polyhedrin promoter) adds TEV-protease-removable N-terminal His10-tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites
    Depositor
    Debra Hansen
    Article
    Olson et al Protein Expr Purif. 2021 May 7:105890. d
    Type
    Empty backbone
    Use
    Tags
    TEV-protease-cleavable His10-tag
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    159427-plasmid-map-sequence-id-333995

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Showing: 261 - 280 of 21779 results