We narrowed to 5,304 results for: pcDNA3.1(+)

1. pcDNA3.1.H1-NP

   Plasmid

   #134367

   Purpose

   Nanoluc complementation assay. Expression of histamine receptor H1 fused at C termimus with Natural peptide (NP) of NanoLuc. Addition of the Flag epitope at N terminus of H1 receptor.

   Depositor

   Julien Hanson

   Article

   Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

   Insert

   H1-NP (HRH1 Human)
   

   Use

   Tags

   Flag and natural peptide of nanoluciferase

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   T7

   Available Since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1_27F3_hFc

   Plasmid

   #199619

   Purpose

   Encodes DARPin-hFc 27F3 for mammalian expression and column purification

   Depositor

   Shiva Tyagarajan

   Article

   Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

   Insert

   DARPin-hFc 27F3
   

   Use

   Tags

   hFc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1_hMed25

   Plasmid

   #206958

   Purpose

   The ORF of human Med25 cDNA amplified from the RT human liver RNA was cloned into pGEMTE vector and then subclone at XhoI/XbaI sites of the pcDNA3.2(+), the mammalian expression vector.

   Depositor

   Reza Zolfaghari, Catharine Ross

   Insert

   Homo sapiens mediator complex subunit 25 (MED25), transcript variant 1, mRNA (MED25 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1_hE2F7mg

   Plasmid

   #73073

   Purpose

   human E2F7 minigene (exons 11,12,13/introns cassette)

   Depositor

   Stefan Stamm

   Article

   Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

   Insert

   E2F7 (E2F7 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   partial gene, exons 11,12,13 spaced by introns

   Promoter

   CMV

   Available Since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1dsRluc

   Plasmid

   #68053

   Purpose

   expresses destabilized Renilla Luciferase

   Depositor

   Christopher Sullivan

   Article

   Seo et al J Virol. 2008 Oct;82(20):9823-8. doi: 10

   Insert

   Renilla luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 11, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

6. HfACR1_EYFP_pcDNA3.1

   Plasmid

   #161026

   Purpose

   Expresses strongly red-shifted HfACR1 in mammalian cells

   Depositor

   John Spudich

   Article

   Govorunova et al Proc Natl Acad Sci U S A. 2020 Sep 15;11

   Insert

   HfACR1
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3.1_CoumRS

   Plasmid

   #64882

   Purpose

   Expresses Coumarinyl- tRNA synthetase

   Depositor

   Sebastián Brauchi

   Article

   Steinberg et al bioRxiv, 10.1101/065227

   Insert

   Coum_RS
   

   Use

   Tags

   Flag

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. A1ACR1_EYFP_pcDNA3.1

   Plasmid

   #161025

   Purpose

   Expresses strongly red-shifted A1ACR1 in mammalian cells

   Depositor

   John Spudich

   Article

   Govorunova et al Proc Natl Acad Sci U S A. 2020 Sep 15;11

   Insert

   A1ACR1
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. CRAF_S259A_pcDNA3.1

   Plasmid

   #86505

   Purpose

   Expresses CRAF_S259A in mammalian cells

   Depositor

   Dustin Maly

   Article

   Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

   Insert

   CRAF (RAF1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S259A

   Promoter

   CMV

   Available Since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1hLCAD_FLAG

    Plasmid

    #24491

    Depositor

    Eric Verdin

    Article

    Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

    Insert

    Long chain acyl-CoA dehydrogenase (ACADL Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 23, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3.1_hSMAD4

    Plasmid

    #212710

    Purpose

    The ORF of human SMAD4, variant 1 cDNA amplified from the RT human liver RNA was cloned at the NheI/HindIII sites of the pcDNA3.1(+), the mammalian expression vector.

    Depositor

    Reza Zolfaghari, Catharine Ross

    Insert

    Homo sapiens SMAD4 (SMAD4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1mLCAD_FLAG

    Plasmid

    #24484

    Depositor

    Eric Verdin

    Article

    Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

    Insert

    Long chain acyl-CoA dehydrogenase (Acadl Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 23, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3.1_27B3_hFc

    Plasmid

    #199618

    Purpose

    Encodes DARPin-hFc 27B3 for mammalian expression and column purification

    Depositor

    Shiva Tyagarajan

    Article

    Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

    Insert

    DARPin-hFc 27B3
    

    Use

    Tags

    hFc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1_SNORD27h

    Plasmid

    #73066

    Purpose

    expression clone for human SNORD27 (C/D box snoRNA U27)

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

    Insert

    SNORD27 (SNORD27 Human)
    

    Use

    Tags

    no tag

    Expression

    Mammalian

    Mutation

    expression cassette for SNORD27

    Promoter

    CMV

    Available Since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1dsLuc2CP

    Plasmid

    #68054

    Purpose

    expresses destabilized Firefly Luciferase

    Depositor

    Christopher Sullivan

    Article

    Seo et al J Virol. 2008 Oct;82(20):9823-8. doi: 10

    Insert

    Firefly luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

16. AlACR1_EYFP_pcDNA3.1

    Plasmid

    #161022

    Purpose

    Expresses strongly red-shifted AlACR1 in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al Proc Natl Acad Sci U S A. 2020 Sep 15;11

    Insert

    AlACR1
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. T1ACR1_EYFP_pcDNA3.1

    Plasmid

    #161027

    Purpose

    Expresses strongly red-shifted T1ACR1 in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al Proc Natl Acad Sci U S A. 2020 Sep 15;11

    Insert

    T1ACR1
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1_SNORD27h_2ntmut

    Plasmid

    #73067

    Purpose

    expression clone for human mutated SNORD27 (C/D box snoRNA U27)

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

    Insert

    SNORD27 (E2F7 Human)
    

    Use

    Tags

    no tag

    Expression

    Mammalian

    Mutation

    2 nt mutation in AS box that binds E2F7 pre-mRNA

    Promoter

    Available Since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3.1 PA-NL

    Plasmid

    #113445

    Purpose

    ProteinA-NanoLuc hybrid fusion control expression vector (for BRET and LuC normalization)

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Insert

    ProteinA-NanoLuc
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. CRAF_S29A_pcDNA3.1

    Plasmid

    #86506

    Purpose

    Expresses CRAF_S29A in mammalian cells [Edit]

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

    Insert

    CRAF (RAF1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S29A

    Promoter

    CMV

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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