We narrowed to 475 results for: piggybac vectors

1. YX022 (PB)EF1a-LIDARsensor (hygR)

   Plasmid

   #233431

   Purpose

   EF1a-driven LIDAR reporter RNA

   Depositor

   Xiaojing Gao

   Article

   Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

   Insert

   mCherry-SGc(stem-1(3xMS2))cSG-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   EF1a

   Available since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. BII-C3H

   Plasmid

   #133386

   Purpose

   Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

   Depositor

   Blair Madison

   Article

   Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. BII-Sh-PnGW-CMV

   Plasmid

   #133366

   Purpose

   Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

   Depositor

   Blair Madison

   Article

   Fischer et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   GFP-NLS
   

   Use

   Tags

   HA-tagged GFP

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CMV

   Available since

   Jan. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. BII-B3HTK

   Plasmid

   #133389

   Purpose

   Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

   Depositor

   Blair Madison

   Article

   Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. BII-ChPtW-eSpCas9

   Plasmid

   #133381

   Purpose

   Piggybac vector for constitutive expression

   Depositor

   Blair Madison

   Article

   Fischer et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   eSpCas9
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   eSpCas9 mutations

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. BII-Sh-PnGW-CMV-Luc

   Plasmid

   #133367

   Purpose

   Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

   Depositor

   Blair Madison

   Article

   Fischer et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   GFP-NLS
   

   Use

   Tags

   HA-tagged GFP

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CMV

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. BII-Sh-PIR702W-Luc

   Plasmid

   #133362

   Purpose

   Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

   Depositor

   Blair Madison

   Article

   Fischer et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   IRFP-702
   

   Use

   Tags

   HA-tagged IRFP

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available since

   Jan. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. BII-Sh-PIR702W

   Plasmid

   #133361

   Purpose

   Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

   Depositor

   Blair Madison

   Article

   Fischer et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   IRFP-702
   

   Use

   Tags

   HA-tagged IRFP

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available since

   Jan. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. BII-Sh-PnGW-Luc

   Plasmid

   #133360

   Purpose

   Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

   Depositor

   Blair Madison

   Article

   Fischer et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   GFP-NLS
   

   Use

   Tags

   HA-tagged GFP

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available since

   Jan. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pB-MultiplexedReporter-MOR170-1

    Plasmid

    #129461

    Purpose

    piggyBac transposon vector encoding the receptor MOR170-1 and the barcoded CRE reporter gene

    Depositor

    Sriram Kosuri

    Article

    Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

    Insert

    MOR170-1 (Olfr895 Mouse)
    

    Use

    Synthetic Biology

    Tags

    LUCY tag, Rho tag

    Expression

    Mammalian

    Mutation

    Promoter

    TRE (Tet-On)

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pB-RTP1S-Integration

    Plasmid

    #129458

    Purpose

    piggyBac transposon vector that Inducibly (Tet-On) expresses RTP1S

    Depositor

    Sriram Kosuri

    Article

    Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

    Insert

    RTP1 (Rtp1 Mouse)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Removal of the first 36 amino acids of the protein

    Promoter

    TRE (Tet-On)

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. ePB-Bsd-CAG-LoxP-dsRed_PA-LoxP-SV40-NLS-NG-T2A-MCS:

    Plasmid

    #179585

    Purpose

    ePiggyBac vector that has a blasticidin selectable cassette and a LoxP exchangeable dual colors cassette (Red module-dsRed/Green module-NeonGreen)

    Depositor

    Ali Hemmati Brivanlou

    Article

    De Santis et al Nat Commun. 2021 Nov 19;12(1):6768. doi:

    Insert

    NLS-NG-T2A-hSHH
    

    Use

    Piggybac

    Tags

    NLS-NG-T2A

    Expression

    Mutation

    Promoter

    CAGp

    Available since

    Feb. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. BII-Sh-FUCCI

    Plasmid

    #133363

    Purpose

    Piggybac vector for shRNA-mediated knockdown, co-expressed with FUCCI cell cycle reporter

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    Clover-Geminin-IRES-mKO2-Cdt1
    

    Use

    Tags

    HA-tagged Clover-Geminin

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPB-PNGT3-d2EGFP_CMV-mCherry

    Plasmid

    #213765

    Purpose

    Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.

    Depositor

    Gaetano Gargiulo

    Article

    Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

    Insert

    PNGT#3-d2EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PB-CT3G-CERP2-MG-IRES2-mNeonGreen

    Plasmid

    #175508

    Purpose

    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (CMVe-hEF1a-driven rtTA and puromycin resistance)

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G-mCMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAC150-RFP-EGFP-LGALS3

    Plasmid

    #175778

    Purpose

    PiggyBac Vector expressing GFP-RFP-LGALS3

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    LGALS3 (LGALS3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PB-CT3G-CERP2-MG-IRES2-mTagBFP2

    Plasmid

    #175509

    Purpose

    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mTagBFP2 (CMVe-hEF1a-driven rtTA and puromycin resistance)

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G-mCMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AgDD

    Plasmid

    #78289

    Purpose

    create protein aggregates in living cells

    Depositor

    Thomas Wandless

    Article

    Miyazaki et al Nat Commun. 2016 May 27;7:11689. doi: 10

    Insert

    NES-L106P-sfGFP (FKBP1A Human)
    

    Use

    Tags

    MLALKLAGLDI

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. PB-hEF1A-MG-U6-sgRNA

    Plasmid

    #175505

    Purpose

    All-in-one piggyBac transposon destination vector for hEF1a expression of Mega Gate cloned elements and hU6 expression of Golden Gate cloned guide RNAs

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hEF1a, U6

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. piggyFlex

    Plasmid

    #218234

    Purpose

    A piggyBac transposon-based gRNA expression vector, to allow for genomic integration and stable expression of gRNAs. Contains both antibiotic (puromycin) and fluorophore (GFP) markers.

    Depositor

    Jay Shendure

    Article

    Chardon et al bioRxiv [Preprint]. 2023 Mar 28:2023.03.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge