We narrowed to 15,623 results for: grna

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1781_pAAV-U6-Ai9-Sa-gRNA1-CB-SACas9-HA-OLLAS-spA_C

Plasmid

#135979

Purpose

gRNA against the left loxP site of the Ai9 allele

Depositor

William Lagor

Insert

Ai9 gRNA1

Use

AAV, CRISPR, and Mouse Targeting

Tags

HA

Promoter

U6

Available Since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pSpCas9nucl-T2A-mCherry - BAKgRNA1- BAKgRNA2

Plasmid

#167295

Purpose

Plasmid encoding for 2 gRNAs targeting the human BAK gene and a CMV driven nuclease Cas9 followed by self-cleaving mCherry

Depositor

Agnel Sfeir

Article

Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

Insert

BAK (BAK1 Human)

Expression

Mammalian

Available Since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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pmCherry-U6-empty

Plasmid

#140580

Purpose

Expresses a gRNA and mCherry in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Type

Empty backbone

Use

CRISPR

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ131C

Plasmid

#69284

Purpose

Golden Gate entry vector; 1st gRNA under OsU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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EZH2 E9.3 gRNA

Plasmid

#90684

Purpose

3rd generation lentiviral gRNA plasmid targeting human EZH2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

EZH2 (Guide Designation E9.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pCRISPRi_Mxi1_yl_NHEJ

Plasmid

#91249

Purpose

CRISPRi vector for Yarrowia lipolytica, repressing KU70 and KU80 for enhanced HR

Depositor

Ian Wheeldon

Article

Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.

Inserts

Codon optimized dCas9-Mxi1
KU70 sgRNA expression cassette
KU80a sgRNA expression cassette
KU80b sgRNA expression cassette

Use

CRISPR and Synthetic Biology

Expression

Yeast

Promoter

SCR1'-tRNA and UAS1B8-TEF(136)

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pYPQ132B

Plasmid

#69282

Purpose

Golden Gate entry vector; 2nd gRNA under AtU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pYPQ131B

Plasmid

#69281

Purpose

Golden Gate entry vector; 1st gRNA under AtU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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AAVi U6_gRNA CMV_SadCas9-KRAB

Plasmid

#214609

Purpose

All-in-one AAVi plasmid expressing S. aureus dCas9-KRAB with sgRNA cassette

Depositor

Ralf Gilsbach

Article

Laurette et al Circ Res. 2023 Dec 22. doi: 10.1161/CIRC

Inserts

gRNA
SadCas9

Use

AAV

Tags

NP NLS and SV40 NLS

Expression

Mammalian

Mutation

D10A, N580A

Promoter

CMV and U6

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRDA_078

Plasmid

#158582

Purpose

Lentiviral expression of BE4max and customizable gRNA

Depositor

John Doench, David Root

Article

Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Mutation

Cas9 D10A

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ132C

Plasmid

#69285

Purpose

Golden Gate entry vector; 1st gRNA under OsU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pMM131

Plasmid

#127214

Purpose

BeYDV replicon with constitutive Luc expression, AtSTM, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc, AtSTM, sgRNA

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ133B

Plasmid

#69283

Purpose

Golden Gate entry vector; 3rd gRNA under AtU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-T2A-Puro_h53BP1_gRNA_D

Plasmid

#110302

Purpose

Expresssion of Cas9-T2A-puromycin resistant gene and a gRNA targeting exon 10 of human 53BP1

Depositor

Chris Kok-Lung Chan

Article

Tiwari et al Nat Commun. 2018 Feb 14;9(1):677. doi: 1

Insert

p53-binding protein 1 (TP53BP1 Human)

Expression

Mammalian

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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PRKDC gRNA (BRDN0001146589)

Plasmid

#77861

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKDC

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKDC (PRKDC Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pC0051-W85X REPAIR targeting guide cloned into pC0043

Plasmid

#103867

Purpose

Guide RNA expression plasmid for dPspCas13b that can be used to direct ADAR deaminase domain activity to the W85X mutation in the luciferase reporter plasmid pC0038

Depositor

Feng Zhang

Article

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Insert

W85X targeting guide RNA

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 28, 2017

Availability

Academic Institutions and Nonprofits only

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gRNA-CXCR4(90)

Plasmid

#98962

Purpose

Mammalian expression plasmid for CRISPR gRNA targeting human CXCR4 exon 2

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CXCR4 (CXCR4 Human)

Use

CRISPR

Expression

Mammalian

Mutation

Encodes a gRNA for CRISPR-mediated targeting of C…

Promoter

U6

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pMM135

Plasmid

#127216

Purpose

BeYDV replicon with constitutive Luc expression, WUS2, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc, WUS2, sgRNA

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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LentiGuide-TagBFP-2A-BSD

Plasmid

#167929

Purpose

Lentiviral gRNA expression with TagBFP fluorescence and blasticidin selection marker.

Depositor

Ronald Germain

Article

Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6, EF1a

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pX330A-1x5

Plasmid

#58769

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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