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We narrowed to 26,573 results for: Cat

Showing: 2801 - 2820 of 26573 results

  1. pLCHKO_TK1_HPRT1_Lb_1

    Plasmid
    #155059
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA targeting TK1 using SpCas9 and HPRT1 using LbCas12a (CHyMErA system)
    Depositor
    Jason Moffat, Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA targeting TK1 using SpCas9 and HPRT1 using LbCas12a
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155059-plasmid-map-sequence-id-308442

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  2. pLCHKO_MDM4_exon_deletion_3

    Plasmid
    #155075
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Jason Moffat, Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    155075-plasmid-map-sequence-id-308578

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  3. pMP108 Nanog-Venus-2a-mCherry

    Plasmid
    #159743
    Purpose
    Nanog targeting vector to insert fluorescent reporter of protein and gene expression levels from endogenous locus in mouse embryonic stem cells. Please see depositor comments for more detail.
    Depositor
    Sergi Regot
    Article
    Pokrass et al Dev Cell. 2020 Nov 9;55(3):328-340.e5. d
    Insert
    Nanog homeobox (Nanog Mouse)
    Use
    Mouse Targeting
    Tags
    Venus-2a-mCherry
    Expression
    Mutation
    Promoter
    Available since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    159743-plasmid-map-sequence-id-330621

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  4. Epac-S-H96

    Plasmid
    #170342
    Purpose
    CFP(UST)Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    van der Krogt et al PLoS ONE. 2008 . 3(4):e1916.
    Insert
    CFP(UST)Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    170342-plasmid-map-sequence-id-344641

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  8. delNC C-His Ana GvpC in pET28a

    Plasmid
    #85736
    Purpose
    Expresses a truncated His-tagged GvpC from Anabaena flos-aquae without the N-and C-terminal regions in E.coli
    Depositor
    Mikhail Shapiro
    Article
    Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi
    Insert
    Gas vesicle protein C (truncated and his-tagged)
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Deletion of N and C terminal regions, addition of…
    Promoter
    T7
    Available since
    March 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    85736-plasmid-map-sequence-id-174840

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  9. pIRES:mTREK-1(K271Q)

    Plasmid
    #133273
    Purpose
    Mammalian expression vector. It will generate the mouse TREK-1 channel full lenght K271Q mutant fused to a N-terminal HA tag
    Depositor
    Dan Minor
    Article
    Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d
    Insert
    Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)
    Use
    Tags
    HA
    Expression
    Mammalian and Yeast
    Mutation
    K271Q
    Promoter
    Available since
    April 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    133273-plasmid-map-sequence-id-292434

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  11. pD10AH840AhCas9 (GB1041)

    Plasmid
    #68207
    Purpose
    Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part
    Depositor
    Diego Orzaez
    Article
    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.
    Insert
    Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed; human codon optimis…
    Promoter
    Available since
    March 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    68207-plasmid-map-sequence-id-135575

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  19. pKT-CNP-inact

    Plasmid
    #211804
    Purpose
    Expresses inactive 2',3'-cyclic nucleotide phosphodiesterase catalytic domain (catalytic residues mutated)
    Depositor
    Emily Weinert
    Article
    Fontaine et al Biochem J. 2018 Apr 30;475(8):1491-1506.
    Insert
    2',3'-cyclic nucleotide phosphodiesterase catalytic domain (Cnp Rat)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    H73L H153L
    Promoter
    tet
    Available since
    Dec. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    211804-plasmid-map-sequence-id-417773

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  20. pET15b MOSPD2 RD/LD [282-490]

    Plasmid
    #186470
    Purpose
    Expression of human MOSPD2 (MSP domain from residue 282 to 490; mutant R404D/L406D, unable to bind FFAT motifs), fused to a 6HIS tag, in bacteria
    Depositor
    Catherine Tomasetto
    Article
    Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017
    Insert
    MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
    Use
    Tags
    6HIS
    Expression
    Bacterial
    Mutation
    MSP domain [282-490]
    Promoter
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    186470-plasmid-map-sequence-id-367335

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Showing: 2801 - 2820 of 26573 results