We narrowed to 6,450 results for: siae
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Plasmid#202485PurposeExpresses sulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) constitutivelyDepositorInsertsulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) (ERV1 Budding Yeast, Escherichia coli)
ExpressionBacterialPromoterEM7Available SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMVtight-UPRT-T2A-RFP-IRES-CD
Plasmid#126677PurposeDoxycycline inducible expression of UPRT and CD along with reporter RFPDepositorInsertUPRT-T2A-RFP-IRES-CD (FCY1 Budding Yeast, Toxoplasma gondii)
Promotertight TREAvailable SinceJune 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
HDAC7 Flag
Plasmid#13824PurposeMammalian expression of human histone deacetylase 7 with flag tagDepositorAvailable SinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pHDR-eGFP (pXK-1647)
Plasmid#208824PurposeThe HDR-based surrogate reporter for validating HDR-editing activity of different gene editing tools as well as for helping to screen HDR-editing positive cells. Key Construct: CAG-PuroR-CMV-eGFP*(HDRDepositorTypeEmpty backboneExpressionMammalianPromoterCMVAvailable SinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2
Plasmid#74009PurposeTissue-specific knock-out in zebrafish, Cas9 and GFP driven by a UAS promoterDepositorInserts5x UAS
Cas9
T2A
GFP
U6 promoter
UseCRISPRAvailable SinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
SNAP-OMP25
Plasmid#141149PurposeFluorescent labeling of the outer mitochondrial membrane (OMM) in mammalian cellsDepositorAvailable SinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAEL010097-Cas9
Plasmid#100707PurposeThe promoter of AAEL010097 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegyptiDepositorInsertsAAEL010097-hspcas9-GFP
Opie2-dsRed
UseCRISPRTagsGFP and dsREDExpressionInsectPromoterAAEL010097 and Opie2Available SinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGA-blue
Plasmid#196336PurposeEasy-MISE toolkit Level 0 destination plasmid for parts cloning with Esp3I-based Golden Gate Assembly; allowing fast postitive clones test with AmilCP chromoprotein-based blue/white screeningDepositorTypeEmpty backboneUseSynthetic Biology; Golden gate assembly backboneAvailable SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAct-FRT-stop-FRT3-FRT-FRT3-Gal4 attB
Plasmid#52889Purpose"CoinFLP-Gal4" - Expresses Gal4 from actin promoter downsteam of transcriptional stop. Recombination by FLP excises FRT-FRT pair to express Gal4, or FRT3-FRT3 pair to prevent Gal4 expressionDepositorInsertGal4 (GAL4 Budding Yeast)
ExpressionInsectAvailable SinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFlp-Gm
Plasmid#222335PurposeExpresses Flp and gentamicin to enable excision of antibiotic resistance cassettes; flp-cl-sacB-pL1V-Lv1-gent-pBBR1-ELT3DepositorAvailable SinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFlp-Km
Plasmid#222334PurposeExpresses Flp and kanamycin to enable excision of antibiotic resistance cassettes; flp-cl-sacB-pL1V-Lv1-neo-pBBR1-ELT3DepositorAvailable SinceAug. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AKAR4-NES
Plasmid#64727PurposeCytosol-targeted, FRET-based PKA activity reporter.DepositorInsertAKAR4-NES
TagsCerulean, NES, and cpVenus[E172]ExpressionMammalianPromoterCMVAvailable SinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCfB2904(XI-3 MarkerFree)
Plasmid#73276PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-3 (Chr XI: 93378..94567)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPBbsr2-4031NES
Plasmid#105241PurposeA Förster resonance energy transfer (FRET) biosensor for AMPK activity with cyan and yellow fluorescent protein.DepositorInsertAMPK-EV
ExpressionMammalianPromoterCAGGSAvailable SinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGA-red-maxi
Plasmid#196337PurposeEasy-MISE toolkit Level 1 destination plasmid for cassette cloning with BsaI-based Golden Gate Assembly; allowing fast postitive clones test with mRFP1 fluorescent protein-based red/white screeningDepositorTypeEmpty backboneUseSynthetic Biology; Golden gate assembly backboneAvailable SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSNR52-sgTEF1
Plasmid#46922PurposeYeast CEN/ARS vector (Ura3) that contains sgRNA controlled by SNR 52 promoter, targeting endogenous TEF1 promoterDepositorAvailable SinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGal4AD-CIB1
Plasmid#28245PurposeExpresses Gal4 Activation Domain fused to CIB1 for blue-light-induced transcription activationDepositorAvailable SinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pScRad52-SpCas9 (pXK-1646)
Plasmid#208823PurposeCRISPR/Rad52-Cas9 expression vector for Animal gene editing, harboring the ScRad52-SpCas9 and a VEGFA.gRNA expression cassette. Key Construct: hU6-VEGFa.gRNA-CMV-ScRad52-SpCas9.DepositorTypeEmpty backboneUseCRISPR; Cas9TagsRad52ExpressionMammalianPromoterCMVAvailable SinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
yHS1-M7A
Plasmid#159151PurposeCytosolic labile heme reporterDepositorInsertyHS1-M7A
ExpressionYeastMutationMutated Met 7 of the cyt b562 module of yHS1 to A…PromoterGPDAvailable SinceSept. 21, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTW040
Plasmid#64769PurposeFor integration of Cre recombinase at the yeast HIS3 locus orthe CYC1 terminatorDepositorInsertCre-EBD78
Tagsfused to the human Estrogen Binding Domain (EBD),…ExpressionYeastPromoterTDH3Available SinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRcCMV myc-Plk1 wt (Nigg RG6)
Plasmid#41160DepositorAvailable SinceMarch 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNES-EGFP-C1-flex-PABD-Spo20
Plasmid#220081PurposeSpo20-PABD PA biosensor with a flexible linker to aid in nuclear exportDepositorInsertSpo20 (SPO20 Budding Yeast)
TagsX. leavis map2k1.L(32-44)-EGFP-SGGGSGGSExpressionMammalianMutationamino acids 51-91PromoterCMVAvailable SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CKAR2
Plasmid#123348PurposeImproved FRET-based biosensor for monitoring Protein Kinase C activity.DepositorInsertCKAR2
Tags6xHIS, Cerulean3, T7 tag (gene 10 leader), Xpress…ExpressionMammalianPromoterCMVAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS415-TEF1pr-MFA-NFAPoptim
Plasmid#221090PurposeFAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) + MFA1 signal sequence to help target constructs to the ERDepositorTypeEmpty backboneExpressionYeastAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSS146
Plasmid#64770PurposeFor integration of Cre recombinase at the yeast URA3 locus orthe CYC1 terminatorDepositorInsertCre-EBD78
Tagsfused to the human Estrogen Binding Domain (EBD),…ExpressionYeastPromoterTDH4Available SinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCPP3373
Plasmid#128718PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopQ1-1
ExpressionBacterialMutationSynonymous point mutation in the wobble position…Available SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYES260-sc.eEF2 K509A
Plasmid#85120Purposeyeast expression of mutant EEF2DepositorTags6xHisExpressionYeastMutationK509APromoterGAL1Available SinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS413-TEF1pr-MFA-NFAPoptim
Plasmid#221095PurposeFAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) + MFA1 signal sequence to help target constructs to the ERDepositorTypeEmpty backboneExpressionYeastAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSJ1726 (pFA6-NATMX-CDC42pr-KAR2SS-GFP1-10)
Plasmid#86421PurposeN-terminal PCR tagging cassette for yeast codon optimized split GFP1-10 with CDC42 promoter and KAR2 signal sequenceDepositorInsertCDC42pr-KAR2SS-GFP1-10
TagsGFP1-10ExpressionYeastAvailable SinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMC234r-ccdB/TPK2
Plasmid#176179Purposeyeast MoClo level-0 part plasmid type 234r containing toxic gene dropout cassette (ccdB and TPK2)DepositorInsertdropout cassette containing toxic genes ccdB and TPK2
UseSynthetic BiologyAvailable SinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAEL005635-Cas9
Plasmid#100608PurposeThe promoter of AAEL005635 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegyptiDepositorInsertsAAEL005635-hspcas9-GFP
Opie2-dsRed
UseCRISPRTagsGFP and dsREDExpressionInsectPromoterAAEL005635 and Opie2Available SinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCas9-EcRT-GAL-Hyg
Plasmid#232091PurposeYeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.DepositorInsertSpCas9 and Ec86 reverse transcriptase
UseCRISPRExpressionYeastMutationEc86 reverse transcriptase is codon optimized for…PromoterGAL1-GAL10Available SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTP-PCR C3
Plasmid#189620PurposeCloned Reduced Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteriaDepositorInsertReduced Mitochondrial Genome of Thalassiosira pseudonana
UseSynthetic Biology; Diatom expressionExpressionBacterial and YeastMutationRemoved repeat region, 10-kbp deletion, and minor…Available SinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAV10.F3
Plasmid#63199PurposeBioBrick pSB1C3 backbone (Cm), HIS3, RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. Integration into chrVI: 97873-98803 (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACDura3
Plasmid#138711PurposeExpresses group II intron Ll.LtrB and IEP (LtrA) from Lactoccus lactis under control of T7 promoter. Ll.LtrB is retargeted to insert into 635s locus of LacZ gene and ura3 gene is cloned inside.DepositorInsertura3 gene (URA3 Budding Yeast)
ExpressionBacterialMutationoptimized ura3 gene for expression in Escherichia…PromoterpEM7 promoterAvailable SinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pST1374-GCN4-D10A
Plasmid#113022PurposeExpresses GCN4-D10A in mammalian cellsDepositorAvailable SinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pProEx Ypt1(S17C) CL
Plasmid#224265PurposeProtein purification of Ypt1 (S17C)DepositorAvailable SinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB2909(XII-5 MarkerFree)
Plasmid#73281PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-5 (Chr XII: 839226..840357)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV10.KH
Plasmid#63208PurposepCACS backbone (Amp), Hyg, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCas9-EcRT-GAL-HIS3
Plasmid#232093PurposeYeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.DepositorInsertSpCas9 and Ec86 reverse transcriptase
UseCRISPRExpressionYeastMutationEc86 reverse transcriptase is codon optimized for…PromoterGAL1-GAL10Available SinceFeb. 25, 2025AvailabilityAcademic Institutions and Nonprofits only