We narrowed to 6,558 results for: siae
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Plasmid#121520PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
ExpressionYeastMutationdeleted amino acids 266-280Available SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAD-Atg17(∆15-106)
Plasmid#121515PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
ExpressionYeastMutationdeleted amino acids 15-106Available SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAD-Atg17(∆186-236)
Plasmid#121519PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
ExpressionYeastMutationdeleted amino acids 186-236Available SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAD-Atg17(∆266-312)
Plasmid#121521PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
ExpressionYeastMutationdeleted amino acids 266-312Available SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRPL28 SpHIS5 (pNTI663)
Plasmid#115433PurposeExpresses wild-type RPL28 from HIS3 yeast plasmidDepositorInsertsExpressionYeastMutationMfeI site in intronPromoterAshbya gospii TEF1 and endogenousAvailable SinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRPL28(Q38P) SpHIS5 (pNTI664)
Plasmid#115434PurposeExpresses Q38P RPL28 from HIS3 yeast plasmidDepositorInsertsExpressionYeastMutationQ38P; MfeI site in intronPromoterAshbya gospii TEF1 and endogenousAvailable SinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRPL28(H39L) SpHIS5 (pNTI665)
Plasmid#115435PurposeExpresses H39L RPL28 from HIS3 yeast plasmidDepositorInsertsExpressionYeastMutationH39L; MfeI site in intronPromoterashbya gospii TEF1 and endogenousAvailable SinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHES 946
Plasmid#112644Purposeblue light inducible cryptochrome based optogenetic expression systemDepositorInsertRTG3AD-GSx7-CIB1-SV40NLS (CIB1 Synthetic, Mustard Weed, Budding Yeast)
ExpressionYeastPromoterADH1Available SinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRPB1–4stop
Plasmid#112824PurposepSMF2 with repeats 8–10 interrupted with 4 repeats containing stop codonsDepositorInsertRPB1 (RPO21 Budding Yeast)
ExpressionYeastMutationpSMF2 with repeats 8–10 interrupted with 4 repeat…PromoterNative promoterAvailable SinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 T178A R181A R182A R186A (N4A)
Plasmid#111940PurposeThis is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the T178A R181A R182A R186A mutationDepositorInsertHsh155 T178A R181A R182A R186A (N4A)
ExpressionBacterial and YeastMutationT178A R181A R182A R186AAvailable SinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 Q773A R775A R776A Q778A (C4A)
Plasmid#111946PurposeThis is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the Q773A R775A R776A Q778A mutationDepositorInsertHsh155 Q773A R775A R776A Q778A (C4A)
ExpressionBacterial and YeastMutationQ773A R775A R776A Q778AAvailable SinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS315Nab3D191stop
Plasmid#110537PurposeExpresses yeast NAB3 using the endogenous promoterDepositorAvailable SinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
p415TEF-NYFP
Plasmid#99555PurposeExpresses Swi1 NQ-YFP to monitor Swi1 state or induce [SWI+].DepositorAvailable SinceAug. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMDC84-ScSEIPIN-Ndelta
Plasmid#96985PurposeExpress yeast SEIPIN gene in plants.DepositorInsertScSEIPIN (SEI1 Budding Yeast)
Tags6xHis and GFPExpressionPlantMutationN-terminal 39 nucleotides were deleted from yeast…Promoter35SAvailable SinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTW094
Plasmid#64757PurposeRecombination-Induced Tag Exchange (RITE) Cassette, T7 to an HA-6xHIS epitope tag, Hygro selectionDepositorInsertRITE cassette
UseRite cassettePromoternoneAvailable SinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMT005
Plasmid#64760PurposeRecombination-Induced Tag Exchange (RITE) Cassette, 2xT7 to an HA-6xHIS epitope tag, Hygro selectionDepositorInsertRITE cassette
UseRite cassettePromoternoneAvailable SinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB812
Plasmid#60160PurposeYeast codon-optimized yellow fluorescent protein (yEVenus) with PEST degron driven by RNR1 promoter.DepositorInsertyEVenus-PEST
TagsPEST degronExpressionYeastPromoterRNR1prAvailable SinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
p413GAL1-Ypd*1-L-IFP-F[2] (Yeast)
Plasmid#52898PurposeYpdI* is fused to the IFP-F[2] by (GGGGS) X 2. Ypd1*/Skn7t: no signal, Ypd1*/Ssk1t: weak signal. Internal ClaI site in Ypd*I. Plasmid confers ampicillin resistance to DH5α.DepositorInsertYpd*1-L-IFP-F[2]
TagsL-IFP-F[2]ExpressionYeastMutationChanged Tryptophane 80 to AlaninePromoterGAL1Available SinceJune 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLG-Nde-Acc
Plasmid#33150DepositorInsertRP51A-synthetic minimal intron (RPS17A Budding Yeast)
TagsLacZExpressionYeastMutationAccI site in intron was cut/filled/ligated to cha…Available SinceDec. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRS416 Gal-RNQ1 L94A-YFP
Plasmid#18692DepositorInsertRnq1 L94A (RNQ1 Budding Yeast)
TagsYFPExpressionYeastMutationLeucine 94 mutated to Alanine (L94A)Available SinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pJC25R1+4stop
Plasmid#17723DepositorInsertSup35R1+4 (SUP35 Budding Yeast)
ExpressionBacterialMutationE.coli expression plasmid for NM-region of Sup35 …Available SinceApril 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAV10
Plasmid#63213PurposepUC19 backbone (Amp), contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. NotI or FseI sites for TU digest.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-LifeAct-FRB-DMVF-181-N
Plasmid#87610PurposeFRB-tagged N-terminal Dronpa fragment for BiFC; localizes to actin cytoskeleton.DepositorInsertLifeAct-FRB-DMVF181-N
Tags17-aa sequence from Abp140 (aka "Lifeact&quo…ExpressionMammalianPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pRS313 - prp8 V1860N
Plasmid#90966Purposeyeast vectorDepositorAvailabilityAcademic Institutions and Nonprofits only -
pRS313 - prp8 V1862D
Plasmid#90967Purposeyeast vectorDepositorAvailabilityAcademic Institutions and Nonprofits only -
pRS313 - prp8 T1865K
Plasmid#90968Purposeyeast vectorDepositorAvailabilityAcademic Institutions and Nonprofits only -
pRS313 - prp8 A1871E
Plasmid#90969Purposeyeast vectorDepositorAvailabilityAcademic Institutions and Nonprofits only -
pRS313 - prp8 T1872E
Plasmid#90970Purposeyeast vectorDepositorAvailabilityAcademic Institutions and Nonprofits only -
pRS313 - prp8 T1861P
Plasmid#90971Purposeyeast vectorDepositorAvailabilityAcademic Institutions and Nonprofits only -
pRS313 - prp8 V1862Y
Plasmid#90972Purposeyeast vectorDepositorAvailabilityAcademic Institutions and Nonprofits only -
pRS313 - prp8 H1863E
Plasmid#90973Purposeyeast vectorDepositorAvailabilityAcademic Institutions and Nonprofits only -
pRS313 - prp8 K1864E
Plasmid#90974Purposeyeast vectorDepositorAvailabilityAcademic Institutions and Nonprofits only -
pRS313 - prp8 N1869D
Plasmid#90975Purposeyeast vectorDepositorAvailabilityAcademic Institutions and Nonprofits only -
pRS313 - prp8 V1870N
Plasmid#90976Purposeyeast vectorDepositorAvailabilityAcademic Institutions and Nonprofits only -
pRS313 - prp8 I1875T
Plasmid#90977Purposeyeast vectorDepositorAvailabilityAcademic Institutions and Nonprofits only -
pDSM-cd-Pfba1p-ACO-Tgpm1
Plasmid#127731PurposeYeast pathway position 4. ACO transcription unit with the FBA1 promoter and GPM1 terminator.DepositorInsertaconitase
UseSynthetic BiologyExpressionYeastPromoterPfba1AvailabilityAcademic Institutions and Nonprofits only -
pDSM-cd-Pykt6-ACO-Tprm9
Plasmid#127732PurposeYeast pathway position 4. ACO transcription unit with the YKT6 promoter and PRM9 terminator.DepositorInsertaconitase
UseSynthetic BiologyExpressionYeastPromoterPykt6AvailabilityAcademic Institutions and Nonprofits only -
pDSM-cd-Ptef1-ACO-Tyol036w
Plasmid#127733PurposeYeast pathway position 4. ACO transcription unit with the TEF1 promoter and YOL036w terminator.DepositorInsertaconitase
UseSynthetic BiologyExpressionYeastPromoterPtef1AvailabilityAcademic Institutions and Nonprofits only -
pDSM-cd-Psptdh3-ACO-Tagtef1
Plasmid#127734PurposeYeast pathway position 4. ACO transcription unit with the S. paradoxus TDH3 promoter and A. gossypii TEF1 terminator.DepositorInsertaconitase
UseSynthetic BiologyExpressionYeastPromoterPsptdh3AvailabilityAcademic Institutions and Nonprofits only -
pHCMM1
Plasmid#167452PurposeExpresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the TEF1 promoterDepositorInsertPTEF1_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1
ExpressionYeastPromoterTEF1AvailabilityAcademic Institutions and Nonprofits only