Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 7,855 results for: 11

Showing: 7001 - 7050 of 7855 results

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pAc5.1B-lambdaN-HA-GST-DmNanos_116-163-V5His6_AC

Plasmid

#148469

Purpose

Insect Expression of DmNanos_116-163

Depositor

Elisa Izaurralde

Article

Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

Insert

DmNanos_116-163 (nos Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-GST-DmNanos_164-236_AC

Plasmid

#148470

Purpose

Insect Expression of DmNanos_164-236

Depositor

Elisa Izaurralde

Article

Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

Insert

DmNanos_164-236 (nos Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-GST-DmNanos_297-381_AC

Plasmid

#148471

Purpose

Insect Expression of DmNanos_297-381

Depositor

Elisa Izaurralde

Article

Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

Insert

DmNanos_297-381 (nos Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpG-DmNanos_116-163-E151AF152AN155A_AB

Plasmid

#148328

Purpose

Bacterial Expression of DmNanos_116-163-E151AF152AN155A

Depositor

Elisa Izaurralde

Article

Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

Insert

DmNanos_116-163-E151AF152AN155A (nos Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpG-DmNanos_116-163-I123M_AB

Plasmid

#148329

Purpose

Bacterial Expression of DmNanos_116-163-I123M

Depositor

Elisa Izaurralde

Article

Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

Insert

DmNanos_116-163-I123M (nos Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpG-DmNanos-opt_50-236-GB1opt_AB

Plasmid

#148331

Purpose

Bacterial Expression of DmNanos-opt_50-236

Depositor

Elisa Izaurralde

Article

Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

Insert

DmNanos-opt_50-236 (nos Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpG-DmNanos-opt_116-163-L127DF130D_AB

Plasmid

#148333

Purpose

Bacterial Expression of DmNanos-opt_116-163-L127DF130D

Depositor

Elisa Izaurralde

Article

Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

Insert

DmNanos-opt_116-163-L127DF130D (nos Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-HsNot1iso1_1833-2361-H1949D_AB

Plasmid

#148335

Purpose

Bacterial Expression of HsNot1iso1_1833-2361-H1949D

Depositor

Elisa Izaurralde

Article

Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

Insert

HsNot1iso1_1833-2361-H1949D (CNOT1 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-HsNot1iso1_1833-2361-V1880E_AB

Plasmid

#148336

Purpose

Bacterial Expression of HsNot1iso1_1833-2361-V1880E

Depositor

Elisa Izaurralde

Article

Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

Insert

HsNot1iso1_1833-2361-V1880E (CNOT1 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-CBS (H65R)

Plasmid

#182924

Purpose

Mammalian expression plasmid for human CBS mutant H65R

Depositor

Philip Eaton

Article

Switzer et al Proc Natl Acad Sci U S A. 2023 Jan 17;12

Insert

Cystathionine Beta-Synthase (CBS Human)

Tags

eGFP

Expression

Mammalian

Mutation

changed histidine 65 to arginine

Promoter

CMV

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pFastBac-M13-6His-KIF1C_R169W-GFP

Plasmid

#185979

Purpose

Baculovirus expression of human KIF1C with R169W mutation and 6xHis and GFP tags.

Depositor

Anne Straube

Article

Siddiqui et al Curr Biol. 2022 Sep 12;32(17):3862-3870.

Insert

KIF1C (KIF1C Human)

Tags

6xHis and eGFP

Expression

Insect

Mutation

R169W - mutation causing HSP (SPG58)

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pnEA-NvG-DmDCP1_1-127_N

Plasmid

#147050

Purpose

Bacterial Expression of DmDCP1_1-127

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmDCP1_1-127 (Dcp1 Fly)

Expression

Bacterial

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmDCP1-Y34A_P

Plasmid

#147214

Purpose

Insect Expression of DmDCP1-Y34A

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmDCP1-Y34A (Dcp1 Fly)

Expression

Insect

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-HA-DmDCP1-N41AS100A-dsRNAres_P

Plasmid

#147215

Purpose

Insect Expression of DmDCP1-N41AS100A-dsRNAres

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmDCP1-N41AS100A-dsRNAres (Dcp1 Fly)

Expression

Insect

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-HA-DmDCP1-Y34F-dsRNAres_P

Plasmid

#147217

Purpose

Insect Expression of DmDCP1-Y34F-dsRNAres

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmDCP1-Y34F-dsRNAres (Dcp1 Fly)

Expression

Insect

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-Mouse-caspase-1/11a

Plasmid

#183391

Purpose

Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

CARD domain of mouse caspase-1 fused to catalytic domain of mouse caspase-11 (Casp1 Synthetic, Mouse)

Use

Retroviral

Tags

Myc

Promoter

MESV

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pKIF1C_R169W-mCherry-FRB

Plasmid

#185984

Purpose

Mammalian expression of human KIF1C with R169W mutation and mCherry and FRB tags.

Depositor

Anne Straube

Article

Siddiqui et al Curr Biol. 2022 Sep 12;32(17):3862-3870.

Insert

KIF1C (KIF1C Human)

Tags

mCherry and FRB

Expression

Mammalian

Mutation

R169W - mutation causing HSP (SPG58) and silent m…

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only
pFastBac-M13-6His-KIF1C_P176L-GFP

Plasmid

#185978

Purpose

Baculovirus expression of human KIF1C with P176L mutation and 6xHis and GFP tags.

Depositor

Anne Straube

Article

Siddiqui et al Curr Biol. 2022 Sep 12;32(17):3862-3870.

Insert

KIF1C (KIF1C Human)

Tags

6xHis and eGFP

Expression

Insect

Mutation

P176L - mutation causing HSP (SPG58)

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-UTX(420-633)-H6

Plasmid

#183773

Purpose

Express pET-UTX(420-633)-H6 in E.coli. Purified pET-UTX(420-633)-H6 was used for generating antibodies in rabbits.

Depositor

Peter Harte

Article

Tie et al Mol Cell Biol. 2012 Jun;32(12):2323-34.

Insert

UTX

Tags

6 His tag at the C-terminus

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pnEA-NpG-DmNanos-opt_116-163-F152E_AB

Plasmid

#148332

Purpose

Bacterial Expression of DmNanos-opt_116-163-F152E

Depositor

Elisa Izaurralde

Article

Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

Insert

DmNanos-opt_116-163-F152E (nos Fly)

Expression

Bacterial

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-HA-DmDCP1-Y34A-dsRNAres_P

Plasmid

#147216

Purpose

Insect Expression of DmDCP1-Y34A-dsRNAres

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmDCP1-Y34A-dsRNAres (Dcp1 Fly)

Expression

Insect

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmDCP1_1-127_A

Plasmid

#145901

Purpose

Insect Expression of DmDCP1_1-127

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmDCP1_1-127 (Dcp1 Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmDCP1_128-372_A

Plasmid

#145902

Purpose

Insect Expression of DmDCP1_128-372

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmDCP1_128-372 (Dcp1 Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmDCP1_1-127_A

Plasmid

#145903

Purpose

Insect Expression of DmDCP1_1-127

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmDCP1_1-127 (Dcp1 Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmDCP1_128-372_A

Plasmid

#145904

Purpose

Insect Expression of DmDCP1_128-372

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmDCP1_128-372 (Dcp1 Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pnYC-NvHN-DmXRN1_1323-1355_N

Plasmid

#147052

Purpose

Bacterial Expression of DmXRN1_1323-1355

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmXRN1_1323-1355 (pcm Fly)

Expression

Bacterial

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pYZ_NAND4_L17_S24_S11_S19_S13

Plasmid

#132732

Purpose

T7 RNAP-driven expression of a 4-input NAND gate RNA with a GFP-ASV reporter that binds to 3WJ repressor trigger indices 24, 11, 19, and 13

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

3WJ_NAND4_L17_S24_S11_S19_S13

Expression

Bacterial

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pYZ_NAND3_L11_S11_S13_S19

Plasmid

#132731

Purpose

T7 RNAP-driven expression of a 3-input NAND gate RNA with a GFP-ASV reporter that binds to 3WJ repressor trigger indices 11, 13, and 19

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

3WJ_NAND3_L11_S11_S13_S19

Expression

Bacterial

Available Since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac-LacI-LAP-HEC1

Plasmid

#114033

Purpose

Production of baculovirus with LacI-LAP-HEC1

Depositor

Geert Kops

Article

Hiruma et al Science. 2015 Jun 12;348(6240):1264-7. d

Insert

LacI-LAP-HEC1 (NDC80 Human)

Expression

Insect

Mutation

none

Promoter

CMV

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET22b-ecDHFR-dCys-T46C

Plasmid

#110543

Purpose

Bacterial expression of E.coli DHFR C85A/C152S/T46C mutant

Depositor

Stephen Benkovic

Article

Liu et al J Am Chem Soc. 2014 Jul 23;136(29):10349

Insert

ecDHFR

Expression

Bacterial

Mutation

T46 mutated to C46, C85 mutated to A85, C152 muta…

Promoter

T7

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET22b-ecDHFR-dCys-L54C

Plasmid

#110542

Purpose

Bacterial expression of E.coli DHFR C85A/C152S/L54C mutant

Depositor

Stephen Benkovic

Article

Liu et al J Am Chem Soc. 2014 Jul 23;136(29):10349

Insert

ecDHFR

Expression

Bacterial

Mutation

L54 mutated to C54, C85 mutated to A85, C152 muta…

Promoter

T7

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSV HA eIF2beta (Pro110-113)

Plasmid

#45899

Depositor

Louise Larose

Article

Kebache et al Proc Natl Acad Sci U S A. 2002 Apr 16;99

Insert

eIF2beta (Eif2s2 Mouse)

Tags

HA

Expression

Mammalian

Mutation

P110A, P113A

Promoter

SV40

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
ZNF668-Flag-D11

Plasmid

#45040

Depositor

Shiaw-Yih Lin

Article

Hu et al Cancer Res. 2011 Oct 15;71(20):6524-34.

Insert

ZNF668 Deletion11 (ZNF668 Human)

Tags

FLAG

Expression

Mammalian

Mutation

contains aa 1-427

Available Since

June 13, 2013

Availability

Academic Institutions and Nonprofits only
ZNF668-Flag-D9

Plasmid

#45038

Depositor

Shiaw-Yih Lin

Article

Hu et al Cancer Res. 2011 Oct 15;71(20):6524-34.

Insert

ZNF668 Deletion9 (ZNF668 Human)

Tags

FLAG

Expression

Mammalian

Mutation

contains aa 1-267

Available Since

June 6, 2013

Availability

Academic Institutions and Nonprofits only
ZNF668-Flag-D8

Plasmid

#45037

Depositor

Shiaw-Yih Lin

Article

Hu et al Cancer Res. 2011 Oct 15;71(20):6524-34.

Insert

ZNF668 Deletion8 (ZNF668 Human)

Tags

FLAG

Expression

Mammalian

Mutation

contains aa 1-190

Available Since

June 6, 2013

Availability

Academic Institutions and Nonprofits only
ZNF668-Flag-D7

Plasmid

#45036

Depositor

Shiaw-Yih Lin

Article

Hu et al Cancer Res. 2011 Oct 15;71(20):6524-34.

Insert

ZNF668 Deletion7 (ZNF668 Human)

Tags

FLAG

Expression

Mammalian

Mutation

contains aa 1-83

Available Since

June 6, 2013

Availability

Academic Institutions and Nonprofits only
ZNF668-Flag-D5

Plasmid

#45034

Depositor

Shiaw-Yih Lin

Article

Hu et al Cancer Res. 2011 Oct 15;71(20):6524-34.

Insert

ZNF668 Deletion5 (ZNF668 Human)

Tags

FLAG

Expression

Mammalian

Mutation

contains aa 428-619

Available Since

June 6, 2013

Availability

Academic Institutions and Nonprofits only
ZNF668-Flag-D4

Plasmid

#45033

Depositor

Shiaw-Yih Lin

Article

Hu et al Cancer Res. 2011 Oct 15;71(20):6524-34.

Insert

ZNF668 Deletion4 (ZNF668 Human)

Tags

FLAG

Expression

Mammalian

Mutation

contains aa 368-619

Available Since

June 6, 2013

Availability

Academic Institutions and Nonprofits only
ZNF668-Flag-D2

Plasmid

#45031

Depositor

Shiaw-Yih Lin

Article

Hu et al Cancer Res. 2011 Oct 15;71(20):6524-34.

Insert

ZNF668 Deletion2 (ZNF668 Human)

Tags

FLAG

Expression

Mammalian

Mutation

contains aa 191-619

Available Since

June 6, 2013

Availability

Academic Institutions and Nonprofits only
ZNF668-Flag-D1

Plasmid

#45030

Depositor

Shiaw-Yih Lin

Article

Hu et al Cancer Res. 2011 Oct 15;71(20):6524-34.

Insert

ZNF668 Deletion1 (ZNF668 Human)

Tags

FLAG

Expression

Mammalian

Mutation

contains aa 84-619

Available Since

June 6, 2013

Availability

Academic Institutions and Nonprofits only
pCDNA3 T7 BimL(S58D,DE)

Plasmid

#25057

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (S58D,DE) (BCL2L11 Human)

Tags

T7

Expression

Mammalian

Mutation

Changed serine 58 to aspartic acid changed leuci…

Available Since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 T7 BimL(S44A,DE)

Plasmid

#25054

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (S44A,DE) (BCL2L11 Human)

Tags

T7

Expression

Mammalian

Mutation

Changed serine 44 to alanine changed leucine 92 …

Available Since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 T7 BimL(T56A,DE)

Plasmid

#25055

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (T56A,DE) (BCL2L11 Human)

Tags

T7

Expression

Mammalian

Mutation

Changed threonine 56 to alanine changed leucine …

Available Since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 T7 BimL(S58A,DE)

Plasmid

#25056

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (S58A,DE) (BCL2L11 Human)

Tags

T7

Expression

Mammalian

Mutation

Changed serine 58 to alanine changed leucine 92 …

Available Since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag BimL(S44D,DE)

Plasmid

#25058

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (S44D,DE) (BCL2L11 Human)

Tags

Flag

Expression

Mammalian

Mutation

Changed serine 44 to aspartic acid changed leuci…

Available Since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag BimL(S58D,DE)

Plasmid

#25061

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (S58D,DE) (BCL2L11 Human)

Tags

Flag

Expression

Mammalian

Mutation

Changed serine 58 to aspartic acid changed leuci…

Available Since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
HM3C NaVSp1 pET24b

Plasmid

#128088

Purpose

Bacterial expression vector for NaVSp1 bacterial sodium channel. It expresses for HIStag-MBP-3C-channel protein

Depositor

Dan Minor

Article

Shaya et al Proc Natl Acad Sci U S A. 2011 Jul 26;10

Insert

NaVSp1p

Tags

3C Cleavage, His Tag, and MBP

Expression

Bacterial

Availability

Academic Institutions and Nonprofits only

Enlarge
pIRES:hTRAAK(W262S)

Plasmid

#133078

Purpose

Mammalian expression vector. It will generate the human TRAAK channel full lenght W262S mutant

Depositor

Dan Minor

Article

Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

Insert

Potassium channel subfamily K member 4 (KCNK4, TRAAK) W262S (KCNK4 Human)

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

Enlarge
pIRES:hTRAAK(G124I)

Plasmid

#133079

Purpose

Mammalian expression vector. It will generate the human TRAAK channel full lenght G124I mutant

Depositor

Dan Minor

Article

Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

Insert

Potassium channel subfamily K member 4 (KCNK4, TRAAK) G124I (KCNK4 Human)

Expression

Mammalian

Mutation

G124I

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV r3.45

Plasmid

#233684

Purpose

An evolved Adeno-associated viral variant, enhances gene delivery and gene targeting in neural stem cells

Depositor

David Schaffer

Article

Jang et al Mol Ther. 2011 Apr;19(4):667-75. doi: 10

Insert

Modified AAV2 Rep, Cap

Use

AAV

Mutation

insertion of LATQVGQKTA (where LA and A are linke…

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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