We narrowed to 142,857 results for: ins

1. AcGFP-MLH1

   Plasmid

   #215120

   Purpose

   Expresses AcGFP-MLH1 in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   MLH1 (MLH1 Human)
   

   Use

   Retroviral

   Tags

   AcGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTWIN1-His6-Ssp-Httex1-7Q

   Plasmid

   #84347

   Purpose

   Expression of the human Huntingtin Exon1 protein containing 7Q in E.coli

   Depositor

   Hilal Lashuel

   Article

   Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

   Insert

   Huntingtin Exon 1 (HTT Human)
   

   Use

   Tags

   N-terminal His6-tagged intein Ssp

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV 3xFLAG-ATG3 C264A

   Plasmid

   #129297

   Purpose

   Expresses 3xFLAG-ATG3 C264A in mammalian cells

   Depositor

   Robin Ketteler

   Article

   Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

   Insert

   Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   changed Cysteine 264 to Alanine

   Promoter

   CMV

   Available since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMSCV-MAVS(IRSp53 pLxIS)

   Plasmid

   #221315

   Purpose

   Expression of MAVS chimera with IRSp53 pLxIS domain in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

   Insert

   MAVS chimera with IRSp53 pLxIS domain (Mavs Mouse)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMSCV-FKBP-STING(pLxIS)

   Plasmid

   #221240

   Purpose

   Expression of STING CTT in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

   Insert

   STING CTT (Sting1 Mouse)
   

   Use

   Retroviral

   Tags

   3xFLAG-4xFKBP

   Expression

   Mutation

   Promoter

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR-HLA-B0702-His

   Plasmid

   #104849

   Purpose

   HLA-B0702 his tagged

   Depositor

   Sébastien Walchli

   Article

   Lampen et al Immunohorizons. 2018 Jul 2;2(6):172-184.

   Insert

   major histocompatibility complex, class I, B (HLA-B Human)
   

   Use

   Entry vector for gateway system

   Tags

   his

   Expression

   Mutation

   Promoter

   none

   Available since

   Dec. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJFA274

   Plasmid

   #49239

   Purpose

   TET1-catalytic domain (CD) expression vector for TALE-TET1 fusion protein to induce targeted demethylation in mammalian cells

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

   Insert

   TET1 tet methylcytosine dioxygenase 1 (TET1 Human, Synthetic)
   

   Use

   TALEN; Tale-tet1 expression vector

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCherry-PNKP

   Plasmid

   #215137

   Purpose

   Expresses mCherry-PNKP in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   PNKP (PNKP Human)
   

   Use

   Retroviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC19-U6-FnCas12a crRNA-BsmBI cassette (BPK4446)

   Plasmid

   #114087

   Purpose

   U6 promoter crRNA entry vector used for all FnCas12a crRNAs (clone spacer oligos into BsmBI cassette)

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   entry vector for FnCas12a crRNAs
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. YA1627: pAAV_hSyn-Dio-paQuasAr3-s-P2A-CheRiff-s

    Plasmid

    #107704

    Purpose

    in vivo voltage imaging

    Depositor

    Adam Cohen

    Article

    Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

    Insert

    paQuasAr3-P2A-CheRiff
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. FokI(inactive)-mCherry-LacI-NLS

    Plasmid

    #215100

    Purpose

    Expresses FokI(inactive)-mCherry-LacI-NLS in mammalian cells.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    FokI(inactive)-mCherry-LacI-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    FokI(inactive) mutant D69A.

    Promoter

    CMV

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-hFnCas12a-NLS(nucleoplasmin)-3xHA (AAS1472)

    Plasmid

    #114089

    Purpose

    CAG promoter expression plasmid for human codon optimized FnCas12a nuclease with C-terminal NLS and HA tag

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized FnCas12a
    

    Use

    Tags

    NLS(nucleoplasmin) and 3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL

    Plasmid

    #119952

    Purpose

    Expresses GPPS and LimS, for the production of GPP and (S)-limonene, in Escherichia coli.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102

    Inserts

    gpps
    limS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N-terminal truncation of 56 amino acid residues (…

    Promoter

    Available since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFastBac SpyTag-β2AR-SpyTag

    Plasmid

    #135620

    Purpose

    Expression of SpyTag-B2AR in insect cell

    Depositor

    Hee-Jung Choi

    Article

    Choi et al Science. 2019 Nov 29;366(6469):1150-1156

    Insert

    Beta 2 adrenergic receptor (ADRB2 Human)
    

    Use

    Tags

    HA-FLAG tag, Spytag, and eGFP-10xHis-R1D4 tag

    Expression

    Insect

    Mutation

    Deleted 1-24 aa, 366-413 aa

    Promoter

    Available since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAVA-LGF2(fs)-Gal11p(fs)

    Plasmid

    #127483

    Purpose

    Negative control for the AVA-seq system. LGF2 (with one nucleotide insertion) (lambda CI associated) and Gal11p (with one nucleotide insertion) (RNAp associated) interaction

    Depositor

    Joel Malek

    Article

    Andrews et al J Biol Chem. 2019 Jul 26;294(30):11549-1

    Insert

    LGF2 (frame shift)-Gal11p (frame shift) (GAL11 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    One nucleotide inserted between lambda CI and LGF…

    Promoter

    Available since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKHH030_sgRB1#2

    Plasmid

    #174145

    Purpose

    Lentiviral vector expressing a sgRNA against the human RB1 gene

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    RB1 sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. MLM3739

    Plasmid

    #49959

    Purpose

    Expresses a mutant TALE-TET1CD (inactive catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene for use as a control

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    3x Flag Tet1CDmut HB-4 (HBB Human)
    

    Use

    TALEN

    Tags

    3x FLAG

    Expression

    Mutation

    catalytic domain of TET1 inactive (H1671Y and D16…

    Promoter

    EF1a

    Available since

    Dec. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

18. MLM3743

    Plasmid

    #49962

    Purpose

    Expresses a mutant TALE-TET1CD (inactive catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene for use as a control

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    3x Flag Tet1CDmut HB-5 (HBB Human)
    

    Use

    TALEN

    Tags

    3x FLAG

    Expression

    Mutation

    catalytic domain of TET1 inactive (H1671Y and D16…

    Promoter

    EF1a

    Available since

    Dec. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

19. MLM3747

    Plasmid

    #49965

    Purpose

    Expresses a mutant TALE-TET1CD (inactive catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene for use as a control

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    3x Flag Tet1CDmut HB-6 (HBB Human)
    

    Use

    TALEN

    Tags

    3x FLAG

    Expression

    Mutation

    catalytic domain of TET1 inactive (H1671Y and D16…

    Promoter

    EF1a

    Available since

    Dec. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pJFA345D4

    Plasmid

    #49238

    Purpose

    TET1-catalytic domain (CD) expression vector for TALE-TET1 fusion protein to induce targeted demethylation in mammalian cells

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    TET1 tet methylcytosine dioxygenase 1 (TET1 Human, Synthetic)
    

    Use

    TALEN; Tale-tet1 expression vector

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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