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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 23,988 results for: nes

Showing: 2801 - 2820 of 23988 results

  1. LP102: pMAGIC (R4-R3) NLS-Sp Cas9-NLS

    Plasmid
    #132927
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2xNLS SpCas9 (nuclease active) for 3 or 4-component MultiSite Gateway Pro assembly.
    Depositor
    Christopher Newgard
    Insert
    humanized SpCas9
    Use
    CRISPR and Synthetic Biology; Pmagic gateway entr…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    132927-plasmid-map-sequence-id-314451

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  8. pMin1

    Plasmid
    #102959
    Purpose
    Mini-transposon system suitable for sequencing, shuttle mutagenesis and gene fusions
    Depositor
    Rainer Haas
    Article
    Kahrs et al Gene. 1995 Dec 29;167(1-2):53-7.
    Type
    Empty backbone
    Use
    Mini-transposon system suitable for sequencing, s…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    102959-plasmid-map-sequence-id-199158

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  14. pETMCSI-T4P_Y320A

    Plasmid
    #122522
    Purpose
    Expression in BL21(DE3) PlysS of mutant T4 DNA polymerase Y320A for easy cloning and mutagenesis
    Depositor
    Gottfried Otting
    Article
    Qi et al PLoS One. 2019 Jan 23;14(1):e0211065. do
    Insert
    gp43 - DNA polymerase (43 Escherichia virus T4)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    tyrosine 320 changed to alanine
    Promoter
    T7 promoter
    Available since
    March 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    122522-plasmid-map-sequence-id-238200

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  17. pBAD-luxOFP

    Plasmid
    #229945
    Purpose
    Bacterial expression of a de novo designed, FRET-based neoLux1.2-CyOFP1 fusion bioluminescent probe (max. Em=588)
    Depositor
    Andy Yeh
    Article
    Chen et al Chem. 2024 Nov 12. doi: 10.1016/j.chempr
    Insert
    luxOFP
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    araBAD
    Available since
    Jan. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    229945-plasmid-map-sequence-id-457163

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  19. pBAD-luxKate

    Plasmid
    #229946
    Purpose
    Bacterial expression of a de novo designed, FRET-based neoLux1.2-mKate2 fusion bioluminescent probe (max. Em=638)
    Depositor
    Andy Yeh
    Article
    Chen et al Chem. 2024 Nov 12. doi: 10.1016/j.chempr
    Insert
    luxKate
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    araBAD
    Available since
    Jan. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    229946-plasmid-map-sequence-id-457155

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  20. pBAD-luxKOk

    Plasmid
    #229944
    Purpose
    Bacterial expression of a de novo designed, FRET-based neoLux1.2-mKOk fusion bioluminescent probe (max. Em=562nm)
    Depositor
    Andy Yeh
    Article
    Chen et al Chem. 2024 Nov 12. doi: 10.1016/j.chempr
    Insert
    luxKOk
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    araBAD
    Available since
    Jan. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    229944-plasmid-map-sequence-id-457161

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Showing: 2801 - 2820 of 23988 results