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pCMV-BE3-2X

Plasmid

#110835

Purpose

CMV expression vector for BE3-2X construct (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3-2X

Expression

Mammalian

Mutation

2 tandem NLS sequences in the XTEN linker

Promoter

CMV

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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PA-mCherry-miniSOG-Cx26-7

Plasmid

#57786

Purpose

Localization: Gap Junctions, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Cx26

Tags

PA-mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
PACT deltaD1

Plasmid

#251698

Purpose

PRKRA (deletion of 1-124) protein expression construct in E. coli

Depositor

Sun Hur

Article

Ahmad et al Nat Commun. 2025 Apr 8;16(1):3325. doi:

Insert

PRKRA (PRKRA Human)

Expression

Bacterial

Mutation

deletion of 1-124

Available Since

April 28, 2026

Availability

Academic Institutions and Nonprofits only

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pLV_CMV_mEmerald-KDEL

Plasmid

#251341

Purpose

ER lumen targeted mEmerald, Lentiviral backbone, CMV promoter.

Depositor

Edward Avezov

Article

Parutto et al Nat Commun. 2026 Feb 11;17(1):2639. doi:

Insert

mEmerald

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2026

Availability

Academic Institutions and Nonprofits only

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CMV_4xmts_HaloTag

Plasmid

#251345

Purpose

Mitochondria matrix targeted HaloTag with 4 mitochondria targeting sequences. CMV promoter.

Depositor

Edward Avezov

Article

Parutto et al Nat Commun. 2026 Feb 11;17(1):2639. doi:

Insert

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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N-term APEX NF1

Plasmid

#253089

Purpose

APEX-tagged NF1 protein on N-term

Depositor

Harish Vasudevan

Article

Vasudevan et al Proc Natl Acad Sci U S A. 2025 Jul 8;122

Insert

NF1 (NF1 Human)

Tags

APEX

Expression

Mammalian

Available Since

April 21, 2026

Availability

Academic Institutions and Nonprofits only

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CRISPRi sgNF1-1

Plasmid

#253096

Purpose

CRISPRi single-guide against NF1

Depositor

Harish Vasudevan

Article

Vasudevan et al Proc Natl Acad Sci U S A. 2025 Jul 8;122

Insert

NF1 (NF1 Human)

Use

CRISPR

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pYO1704

Plasmid

#252704

Purpose

Protein expression of GST-HsLC3B (1-120) in bacteria

Depositor

Roger Williams

Article

van Vliet et al J Cell Biol. 2026 Mar 2;225(3):e20250708

Insert

GST-HsLC3B (1-120) (MAP1LC3B Human)

Expression

Bacterial

Mutation

1-120

Available Since

March 16, 2026

Availability

Academic Institutions and Nonprofits only

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IR InterIR gRNA #5

Plasmid

#247547

Purpose

negative control for Crispri KD

Depositor

Miguel Ramalho-Santos

Article

Ataei et al Genes Dev. 2025 Feb 3;39(3-4):280-298. d

Insert

negative control sgRNA targeting region between inverted repeats of Distal Junction

Use

CRISPR

Expression

Mammalian

Mutation

none

Available Since

March 16, 2026

Availability

Academic Institutions and Nonprofits only

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InterIR gRNA #2

Plasmid

#247546

Purpose

negative control for Crispri KD

Depositor

Miguel Ramalho-Santos

Article

Ataei et al Genes Dev. 2025 Feb 3;39(3-4):280-298. d

Insert

negative control sgRNA targeting region between inverted repeats of Distal Junction

Use

CRISPR

Expression

Mammalian

Mutation

none

Available Since

March 16, 2026

Availability

Academic Institutions and Nonprofits only

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pLKO.1 hygro shRNA mouse Fis1

Plasmid

#252270

Purpose

Knockdown of mouse Fis1

Depositor

David Chan

Article

Nakajima et al Curr Biol. 2026 Mar 9;36(5):1205-1219.e8

Insert

shRNA mouse Fis1 (Fis1 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Feb. 27, 2026

Availability

Academic Institutions and Nonprofits only

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pLKO.1 hygro shRNA mouse Fascin1

Plasmid

#252271

Purpose

Knockdown of mouse Fascin1

Depositor

David Chan

Article

Nakajima et al Curr Biol. 2026 Mar 9;36(5):1205-1219.e8

Insert

shRNA mouse Fascin1 (Fscn1 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Feb. 27, 2026

Availability

Academic Institutions and Nonprofits only

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pLKO.1 hygro shRNA human Fis1

Plasmid

#252272

Purpose

Knockdown of human Fis1

Depositor

David Chan

Article

Nakajima et al Curr Biol. 2026 Mar 9;36(5):1205-1219.e8

Insert

shRNA human Fis1 (FIS1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Feb. 27, 2026

Availability

Academic Institutions and Nonprofits only

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pLKO.1 hygro shRNA human Fascin1

Plasmid

#252273

Purpose

Knockdown of hunam Fascin1

Depositor

David Chan

Article

Nakajima et al Curr Biol. 2026 Mar 9;36(5):1205-1219.e8

Insert

shRNA human Fascin1 (FSCN1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Feb. 25, 2026

Availability

Academic Institutions and Nonprofits only

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pAT1089-(PB-4_EF1α-Blast_2A_rtTA3 [HA-NLS-dCas9-NLS])

Plasmid

#251108

Purpose

Expression of dCas9 component of Casilio-i system

Depositor

Chia-Lin Wei

Article

Taghbalout et al Cancer Cell. 2025 Dec 8;43(12):2191-2205

Insert

dCas9

Tags

HA

Expression

Mammalian

Mutation

N/A

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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pAH_DEST_nHalo-Sec61β-3XFLAG

Plasmid

#250996

Purpose

Expression of Sec61β with N-terminal Halo and C-terminal 3XFLAG tags

Depositor

Anthony Pedley

Article

Sha et al Proc Natl Acad Sci U S A. 2026 Feb 3;123

Insert

SEC61β (SEC61B Human)

Tags

3XFLAG and Halo Tag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

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pmScarlet-i-Sec61β

Plasmid

#250997

Purpose

Expression of Sec61β with an N-terminal mScarlet tag

Depositor

Anthony Pedley

Article

Sha et al Proc Natl Acad Sci U S A. 2026 Feb 3;123

Insert

SEC61β (SEC61B Human)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

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pQβEP1

Plasmid

#210859

Purpose

Express phage Qβ with SARS-CoV Spike protein epitope 1 displayed on the surface that can bind anti-S antibodies under T7 promotor

Depositor

Alain Bopda Waffo

Article

Sanders et al J Biol Chem. 2023 Dec;299(12):105460. do

Insert

SARS-CoV Spike AA441-460 (S )

Tags

No

Expression

Bacterial

Promoter

T7

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pQβEP3

Plasmid

#210861

Purpose

Express phage Qβ with SARS-CoV Spike protein epitope 3 displayed on the surface that can bind anti-S antibodies under T7 promotor

Depositor

Alain Bopda Waffo

Article

Sanders et al J Biol Chem. 2023 Dec;299(12):105460. do

Insert

SARS-CoV Spike AA781-800 (S )

Tags

No

Expression

Bacterial

Promoter

T7

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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TPC2-G-GECO1.2 (F90L)

Plasmid

#240470

Purpose

Lysosomal channel with a low-affinity GECI fused to the C-terminus

Depositor

Antony Galione

Article

Davis et al Cell Calcium. 2023 Dec;116:102801. doi:

Insert

TPC2N2 (TPCN2 Human)

Tags

G-GECO1.2_F90L

Expression

Mammalian

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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