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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 76,970 results for: rest

Showing: 2801 - 2820 of 76970 results

  1. pTP-PCR C3

    Plasmid
    #189620
    Purpose
    Cloned Reduced Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteria
    Depositor
    Bogumil Karas
    Article
    Cochrane et al Biology (Basel). 2020 Oct 26;9(11). pii:
    Insert
    Reduced Mitochondrial Genome of Thalassiosira pseudonana
    Use
    Synthetic Biology; Diatom expression
    Expression
    Bacterial and Yeast
    Mutation
    Removed repeat region, 10-kbp deletion, and minor…
    Available Since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    189620-plasmid-map-sequence-id-379033

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  2. Tol2-CAG::Palmbow

    Plasmid
    #158993
    Purpose
    Multicolor membrane-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing palmitoylated mCherry, mEYFP or mCerulean upon Cre recombination)
    Depositor
    Jean Livet
    Article
    Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10
    Insert
    Palmbow
    Tags
    The default expressed EBFP2 is fused to H2B; oth…
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    158993-plasmid-map-sequence-id-311799

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  5. pSW003-Pc-mNeonGreen

    Plasmid
    #205018
    Purpose
    Broad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with mNeonGreen (codon optimized for P. fluorescens)
    Depositor
    Rosemarie Wilton
    Article
    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:
    Insert
    mNeonGreen
    Expression
    Bacterial
    Mutation
    mNeonGreen is codon optimized for expression in P…
    Promoter
    Pc
    Available Since
    Sept. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    205018-plasmid-map-sequence-id-407997

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  7. C1-mCitrine-FKBP-EZRabd(t567D)

    Plasmid
    #155227
    Purpose
    Acute increase in MPA when used with FRB-FKBP dimerizaton system upon addition with Rapamycin
    Depositor
    Tobias Meyer
    Article
    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210
    Insert
    mCitrine fused to FKBP and the actin BD of Ezrin (T567D), aa552-586 for use with FRB and rapamycin induced dimerization system
    Tags
    mCitrine
    Expression
    Mammalian
    Mutation
    Changed Thr 567 to Asp in original Ezrin
    Available Since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    155227-plasmid-map-sequence-id-310009

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  12. 3xFLAG-dCas9/pMXs-puro

    Plasmid
    #51240
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS (nuclear localization signal)
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available Since
    Aug. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    51240-plasmid-map-sequence-id-410075

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  13. pIND(V5)EcoDam

    Plasmid
    #59201
    Purpose
    Mammalian DamID vector used to fuse gene of interest to V5-tagged E.coli Dam. Can also express Dam alone as negative control.
    Depositor
    Bas van Steensel
    Article
    Vogel et al Genome Res. 2006 Dec;16(12):1493-504. Ep
    Type
    Empty backbone
    Use
    Damid
    Tags
    Dam (DNA adenine methyltransferase) and V5
    Expression
    Mammalian
    Promoter
    Heat Shock Minimal Promoter
    Available Since
    June 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    59201-plasmid-map-sequence-id-247615

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  18. pTet-On-TAP

    Plasmid
    #59017
    Purpose
    Construct allows tetracycline inducible expression of a protein of interest fused to a tandem affinity purification tag (TAP) in toxoplasma gondii.
    Depositor
    David Sibley
    Article
    Etheridge et al Cell Host Microbe. 2014 May 14;15(5):537
    Type
    Empty backbone
    Use
    Toxoplasma expression
    Tags
    Calmodulin Binding Peptide fused with a TEV Cleav…
    Promoter
    RPS13 with Tet Operators Inserted
    Available Since
    Sept. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    59017-plasmid-map-sequence-id-423220

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  20. pRVL-6

    Plasmid
    #104584
    Purpose
    Contains human IgG1 CH1/mutated CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Abolished Fc-mediated immune effector functions
    Depositor
    Daniel Christ
    Article
    Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi
    Insert
    Human IgG1 CH1/CH2/CH3 (mutated, no effector functions) (IGHG1 Human)
    Expression
    Mammalian
    Mutation
    L234A/L235E/G237A and K322A/P331S
    Promoter
    CMV
    Available Since
    Jan. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    104584-plasmid-map-sequence-id-198608

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Showing: 2801 - 2820 of 76970 results