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pTRE-TIGHT-EGFP-donor fw copy

Plasmid

#22074

Depositor

Rudolf Jaenisch

Publication

Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

Insert

TETO-eGFP

Available since

Sept. 28, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCMV5-AML1B

Plasmid

#12426

Purpose

pCMV5-AML1B is the original plasmid used in Meyers et al 1995. However, please note that the insert sequence has now been identified as AML1C.

Depositor

Scott Hiebert

Publication

Meyers et al Mol Cell Biol. 1995 Apr . 15(4):1974-82.

Insert

AML1B (RUNX1 Human)

Expression

Mammalian

Available since

Nov. 17, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pcDNA-SADB19N

Plasmid

#32630

Depositor

Edward Callaway

Publication

Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

Insert

B19N

Expression

Mammalian

Promoter

CMV

Available since

Oct. 26, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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V51 pIRESpuro-GLUE (pGLUE)

Plasmid

#15100

Purpose

tandem-affinity purification plasmid; streptavidin-(streptag) and calmodulin-(CBP) binding affinity tags

Depositor

Randall Moon

Publication

Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

Type

Empty backbone

Tags

CBP tag, HA, and streptag

Expression

Mammalian

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pMK1334

Plasmid

#127965

Purpose

sgRNA expression vector compatible with CROP-Seq and imaging screens

Depositor

Martin Kampmann

Publication

Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

Insert

EF1a-Puro-T2A-2xmycNLS-WPRE-mU6-sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available since

Aug. 24, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pHO4d-Cas9

Plasmid

#67881

Purpose

Cas9nlsHis6 Bacterial Expression construct

Depositor

Michael Nonet

Publication

Fu et al Nucleic Acids Res. 2014 Dec 16;42(22):13

Insert

Cas9

Use

CRISPR

Tags

His6 and T7 epitope

Expression

Bacterial

Promoter

T7

Available since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pYPQ143

Plasmid

#69295

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 3 gRNAs

Depositor

Yiping Qi

Publication

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3 Flag C/EBPa

Plasmid

#66978

Purpose

Expression plasmid for mouse C/EBPa; adds Flag tag to the N-terminus of protein

Depositor

Christopher Vakoc

Publication

Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

Insert

C/EBP alpha (Cebpa Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

July 28, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pFLAG-XBP1u-FLuc

Plasmid

#31239

Depositor

Jimtong Horng

Publication

Jheng et al Cell Microbiol. 2010 Jun . 12(6):796-813

Insert

FLAG-XBP1u-FLuc (XBP1 Human)

Tags

Flag and Fluc

Expression

Bacterial, Insect, and Mamm…

Available since

Aug. 16, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
LeGO-iT2

Plasmid

#27343

Depositor

Boris Fehse

Publication

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

SFFV promoter, IRES-tdTomato

Use

Lentiviral

Expression

Mammalian

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pcDNA3-HA-PHLPP2

Plasmid

#22403

Depositor

Alexandra Newton

Publication

Brognard et al Mol Cell. 2007 Mar 23. 25(6):917-31.

Insert

PHLPP2 (PHLPP2 Human)

Tags

HA

Expression

Mammalian

Available since

Jan. 29, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pPRIME-CMV-GFP-FF3

Plasmid

#11663

Purpose

3rd generation lentiviral transfer vector. pPRIME cloning plasmid with CMV promoter controlling expression of GFP and miR30-based shRNA targeting firefly luciferase

Depositor

Stephen Elledge

Publication

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Insert

FF3

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available since

March 1, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pRK7-FLAG-TSC2

Plasmid

#8996

Depositor

John Blenis

Publication

Tee et al Proc Natl Acad Sci U S A. 2002 Oct 15. 9

Insert

TSC2 (TSC2 Human)

Tags

FLAG

Expression

Mammalian

Available since

Oct. 11, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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3xnls-mScarlet-I

Plasmid

#98816

Purpose

In vivo visualization of the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

3xNLS

Tags

mScarlet-I

Expression

Mammalian

Promoter

CMV

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pmEGFP_a_tubulin_IRES_puro2b

Plasmid

#21042

Depositor

Daniel Gerlich

Publication

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

alpha Tubulin (TUBA1B Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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PB-TO-ASCL1-DLX2

Plasmid

#182307

Purpose

Piggybac Tet-ON plasmid for differentiating hiPSCs into GABAergic neuron via ASCL1 and DLX2 expression

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Publication

van Voorst et al bioRxiv [Preprint]. 2025 Jul 7:2025.06.3

Inserts

Ascl1 (Ascl1 Mouse)
Dlx2 (Dlx2 Mouse)
puroR-T2A-mycNLS-mTagBFP2

Tags

None and T2A-mycNLS-mTagBFP2

Expression

Mammalian

Promoter

EF1a and pTRE3G-bidirectional

Available since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBbA5c-MevT(CO)-T1-MBIS(CO, ispA)

Plasmid

#35152

Depositor

Jay Keasling, Taek Soon Lee

Publication

Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

Insert

AtoB(co)-HMGS(co)-HMGR(co)-pTrc-MK(co)-PMK(co)-PMD-Idi-IspA

Use

Synthetic Biology

Expression

Bacterial

Mutation

R364S in Mevalonate Kinase

Promoter

LacUV5, pTrc

Available since

May 16, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pMSCVpuro-Flag-cMyc T58A

Plasmid

#20076

Depositor

Juan Belmonte

Publication

Aasen et al Nat Biotechnol. 2008 Nov . 26(11):1276-8

Insert

cMyc (MYC Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

T58A

Available since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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ClopHensorN

Plasmid

#50758

Purpose

Mammalian expression vector expressing the ClopHensorN chloride and proton sensor

Depositor

Colin Akerman

Publication

Raimondo et al Front Cell Neurosci. 2013 Nov 13;7:202.

Insert

ClopHensorN

Tags

Strep-II

Expression

Mammalian

Mutation

E2GFP is EGFP-T203Y

Promoter

CAG

Available since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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hrGFP-Keap1

Plasmid

#28025

Depositor

Qing Zhong

Publication

Fan et al Autophagy. 2010 Jul 22. 6(5):.

Insert

Keap1 (KEAP1 Human)

Tags

hrGFP

Expression

Mammalian

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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