We narrowed to 23,509 results for: Sele

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SOX17-3'gRNA

Plasmid

#210465

Purpose

gRNA targeting SOX17 3' terminal for CRISPR-Cas9-mediated knock-in

Depositor

Kai Wang

Article

Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

Insert

SOX17 (SOX17 Human)

Use

CRISPR

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pQTEV-HSPB8

Plasmid

#34710

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

heat shock 22 kDa protein 8 (HSPB8 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pDS170-enNTS1

Plasmid

#199152

Purpose

JZ73, bacterial expression construct expressing thermostabilised rat neurotensin receptor 1 (enNTS1)

Depositor

Daniel Scott

Article

Bumbak et al Cell Rep. 2023 Jan 31;42(1):112015. doi:

Insert

enNTS1 (Ntsr1 Rat)

Tags

10xHis tag, Avi tag, MBP, and muGFP

Expression

Bacterial

Mutation

V208M, C273S, C391S and C393S (over NTS1-B5)

Promoter

Lac/T5

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pBeta-lacZ-attBx2

Plasmid

#124445

Purpose

Plasmid for in vivo enhancer reporter assay. Plasmid contains a β-globin minimal promoter followed by a lacZ reporter gene derived from pRS16, with the entire reporter cassette flanked by attB sites.

Depositor

Yingguang Frank Chan

Article

Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

Type

Empty backbone

Use

Enhancer reporter assay construct

Tags

LacZ

Expression

Mammalian

Promoter

beta globin minimal promoter

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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BB3cK_pGAP_23*_pLAT1_Cas9

Plasmid

#104908

Purpose

hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418

Depositor

Brigitte Gasser

Article

Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

Inserts

HH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)

Use

CRISPR

Expression

Yeast

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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PET-B-Sniper SpCas9-NLS-6xHis

Plasmid

#207377

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-Sniper SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

F539S, M763I, K890N, amino acids 1005-1013 replac…

Promoter

T7

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-Cep72

Plasmid

#136819

Purpose

Mammalian expression of the centrosomal protein Cep72 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Cep72 (CEP72 Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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aMHC-puro rex-neo

Plasmid

#21230

Depositor

Mark Mercola

Article

Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

Insert

alpha myosin heavy chain promoter

Use

Lentiviral

Available Since

Sept. 30, 2009

Availability

Academic Institutions and Nonprofits only

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pHAGE-FLAG-APEX2-MAP1LC3BK51A

Plasmid

#175754

Purpose

Lentiviral vector expressing N-terminally tagged APEX2-FLAG-MAP1LC3B K51A

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

MAP1LC3B (MAP1LC3B Human)

Use

Lentiviral

Mutation

K51A

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-PHP.B3

Plasmid

#103004

Purpose

non-standard AAV2 rep-AAV-PHP.B3 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Article

Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

Insert

Synthetic construct isolate AAV-PHP.B3 VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available Since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCDH-CMV-RPS15A-3xHA plasmid

Plasmid

#192245

Purpose

overexpression vector of RPS15A-3XHA

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

RPS15A CDS without stop coden (RPS15A Human)

Use

Lentiviral

Tags

3xHA

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-PHP.B2

Plasmid

#103003

Purpose

non-standard AAV2 rep-AAV-PHP.B2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Article

Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

Insert

Synthetic construct isolate AAV-PHP.B2 VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available Since

Dec. 15, 2017

Availability

Academic Institutions and Nonprofits only

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PET-Sniper SpCas9-NLS-6xHis

Plasmid

#207374

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

Sniper SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

F539S, M763I, K890N

Promoter

T7

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pQTEV-MAT2B

Plasmid

#34795

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

methionine adenosyltransferase II, beta (MAT2B Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pQTEV-LOC57019

Plasmid

#34811

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

cytokine induced apoptosis inhibitor 1 (CIAPIN1 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pCDNA_B2AR-s-pep

Plasmid

#47438

Purpose

FRET sensor for beta2-adrenergic receptor

Depositor

Sivaraj Sivaramakrishnan

Article

Malik et al J Biol Chem. 2013 Jun 14;288(24):17167-7

Insert

beta2-adrenergic receptor (ADRB2 Human)

Tags

10 nm ER/K helix, HA, mCerulean, mCitrine, and s-…

Expression

Mammalian

Promoter

pCMV

Available Since

Sept. 11, 2013

Availability

Academic Institutions and Nonprofits only
pSIN-Ldha

Plasmid

#175939

Purpose

knockout mouse Ldha

Depositor

Dan Littman

Article

Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

Insert

Ldha gRNA (Ldha Mouse)

Use

Retroviral

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pET21a Wb-iPGM

Plasmid

#180246

Purpose

Bacterial expression plasmid for production of recombinant Wuchereria bancrofti iPGM His10

Depositor

James Inglese

Article

van Neer et al ACS Chem Biol. 2022 Aug 19;17(8):2284-22

Insert

Wuchereria bancrofti iPGM-10His

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCW57.1-Neurod1-3xT7

Plasmid

#200890

Purpose

Inducible lentiviral expression of Neurod1

Depositor

Kai Ge

Article

Xie et al Nat Genet. 2023 Apr 3. doi: 10.1038/s415

Insert

Neurod1 (Neurod1 Mouse)

Use

Lentiviral; Doxycycline inducible

Tags

3xT7

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag p38 beta2 (agf)

Plasmid

#20356

Depositor

Roger Davis

Article

Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

Insert

pDCNA3 Flag p38 beta(agf) (MAPK11 Human)

Tags

Flag

Expression

Mammalian

Mutation

Replace Thr 182 and Tyr 184 with Ala and Phe resp…

Available Since

May 11, 2009

Availability

Academic Institutions and Nonprofits only