We narrowed to 64,855 results for: cel

1. AICSDP-64: FUS-mEGFP

   Plasmid

   #114410

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human FUS

   Depositor

   Allen Institute for Cell Science

   Insert

   FUS Homology Arms with linker-mEGFP (FUS Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mEGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   Sept. 5, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pCDNA3_V5-APEX2-HMOX1

   Plasmid

   #85824

   Purpose

   Expressed N-term tagged APEX2 on HMOX1 in mammalian cell

   Depositor

   Hyun-Woo Rhee

   Article

   Lee et al Cell Rep. 2016 May 11. pii: S2211-1247(1

   Insert

   V5-APEX2-HMOX1 (HMOX1 Human, Synthetic)
   

   Use

   Tags

   APEX2 and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-F

   Available since

   March 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. CRISPseq-BFP-backbone

   Plasmid

   #85707

   Purpose

   Backbone for CRISPR/Cas9 screening with single cell RNA-seq. Lentiviral plasmid for cloning of gRNAs and Unique Guide Index (UGI), with a BFP fluorescent marker. Does NOT include Cas9.

   Depositor

   Ido Amit

   Article

   Jaitin et al Cell. 2016 Dec 15;167(7):1883-1896.e15.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE-IRES-puro-NLS-dLbaCas13a-EGFP-NLS-3xFlag

   Plasmid

   #132408

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

   Insert

   dLbaCas13b
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   R600A; H605A; R1243A; H1248A

   Promoter

   Available since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1(+)_Pmel-1_TCRB

   Plasmid

   #175288

   Purpose

   Expresses Pmel-1 TCR beta chain

   Depositor

   Jeff Hammerbacher

   Article

   Gottschalk et al bioRxiv 2021.11.30.470597

   Insert

   T cell receptor beta chain (Tcrb Mouse)
   

   Use

   Suitable for ivt

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(+)_Pmel-1_TCRA

   Plasmid

   #175287

   Purpose

   Expresses Pmel-1 TCRA alpha chain

   Depositor

   Jeff Hammerbacher

   Article

   Gottschalk et al bioRxiv 2021.11.30.470597

   Insert

   T cell receptor alpha chain (Tcra Mouse)
   

   Use

   Suitable for ivt

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [C12orf35]

   Plasmid

   #227245

   Purpose

   All-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in CHO cell

   Depositor

   Jae Seong Lee

   Article

   Shin et al Metab Eng. 2024 Sep 5;86:12-28. doi: 10.

   Inserts

   Tet-On 3G transactivator protein
   Enhanced green fluorescent protein
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α and pTRE3G

   Available since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti CMV TRE3G BCL2L11 Neo

   Plasmid

   #140747

   Purpose

   Inducibly expresses BIM in mammalian cells

   Depositor

   Scott Dixon

   Article

   Inde et al Cell Rep. 2020 Jul 7;32(1):107845. doi:

   Insert

   BCL2L11 (BCL2L11 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV TRE3G

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pINDUCER21-HOXA9

   Plasmid

   #51302

   Purpose

   Expression of HOXA9 after doxycycline administration

   Depositor

   George Daley

   Article

   Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

   Insert

   homeobox A9 (HOXA9 Human)
   

   Use

   Lentiviral

   Tags

   n/a

   Expression

   Mutation

   n/a

   Promoter

   Available since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. FRAP

    Plasmid

    #1827

    Depositor

    Philip Leder

    Article

    Ornitz et al Mol Cell Biol 1992 Jan;12(1):240-7.

    Insert

    FR1 AP (Fgfr1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 4, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJP-CoV2leader-nLuc-TSS

    Plasmid

    #175430

    Purpose

    Expresses nLuc with 5' leader of CoV2 in mammalian cells.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    nLUC plus 5' CoV2 leader
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. CAGGS-AsCpf1-2A-GFP-U6-INS-sgRNA

    Plasmid

    #159283

    Purpose

    A multicistronic vector with CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA) targeting the human INS locus (sgINS).

    Depositor

    Rudolf Jaenisch

    Article

    Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

    Inserts

    Human INS sgRNA
    Human INS guide RNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA3_HMOX1-V5-APEX2

    Plasmid

    #85823

    Purpose

    Expressed C-term tagged APEX2 on HMOX1 in mammalian cell

    Depositor

    Hyun-Woo Rhee

    Article

    Lee et al Cell Rep. 2016 May 11. pii: S2211-1247(1

    Insert

    HMOX1-V5-APEX2 (HMOX1 Human, Synthetic)
    

    Use

    Tags

    APEX2 and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-F

    Available since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJP-HBB-nLuc

    Plasmid

    #175431

    Purpose

    Expresses nLuc with 5' leader of human beta globin (HBB) in mammalian cells.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    nLUC plus 5' HBB leader
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3 merlin 1-595

    Plasmid

    #11623

    Depositor

    Vijaya Ramesh

    Article

    Xu et al Exp Cell Res. 1998 Jan 10. 238(1):231-40

    Insert

    merlin (NF2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 3, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pCDNA3_V5-APEX2-Sec61B

    Plasmid

    #83412

    Purpose

    Expressed N-term tagged APEX2 on Sec61B in mammalian cell

    Depositor

    Hyun-Woo Rhee

    Article

    Lee et al Cell Rep. 2016 May 11. pii: S2211-1247(1

    Insert

    V5-APEX2-Sec61B (SEC61B Human, Synthetic)
    

    Use

    Tags

    APEX2 and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-F

    Available since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. EID1-pGex-2TK

    Plasmid

    #22689

    Depositor

    William Kaelin

    Article

    Miyake et al Mol Cell Biol. 2000 Dec . 20(23):8889-90

    Insert

    E1A-like inhibitor of differentiation 1 (EID1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    wt cDNA

    Promoter

    Available since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. CRISPseq-mCherry-backbone

    Plasmid

    #85708

    Purpose

    Backbone for CRISPR/Cas9 screening with single cell RNA-seq. Lentiviral plasmid for cloning of gRNAs and Unique Guide Index (UGI), with an mCherry fluorescent marker. Does NOT include Cas9.

    Depositor

    Ido Amit

    Article

    Jaitin et al Cell. 2016 Dec 15;167(7):1883-1896.e15.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG-Bmpr2ΔKinase

    Plasmid

    #163404

    Purpose

    Expresses BMPR-2 lacking the kinase domain in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmpr2 (Bmpr2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    The kinase domain (202-500 aa) is deleted

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. 1119 pBabe puroL HA FKHR AAA

    Plasmid

    #9025

    Depositor

    William Sellers

    Article

    Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

    Insert

    FKHR AAA (FOXO1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T24A S256A S319A

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only