Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 16,254 results for: She

Showing: 5681 - 5720 of 16254 results

  1. LLhScrR-pHG165a 3mut

    Plasmid
    #220278
    Purpose
    Constitutive expression of a chimeric LacI:ScrR transcription repressor
    Depositor
    Liskin Swint-Kruse
    Insert
    LLhScrR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    V52I/Q62S/G120S
    Promoter
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    220278-plasmid-map-sequence-id-436472

    Enlarge

  2. pAN68

    Plasmid
    #164525
    Purpose
    Retroviral expression of CENP-R uORF-1
    Depositor
    Iain Cheeseman
    Insert
    CENP-R uORF-1 (ITGB3BP Human)
    Use
    Retroviral
    Tags
    -6xhis-precission-GFP
    Expression
    Mutation
    Promoter
    Available since
    March 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    164525-plasmid-map-sequence-id-328004

    Enlarge

  3. pAG Retro EGFP Neo

    Plasmid
    #234464
    Purpose
    Control retroviral vector expressing Green Fluorescent Protein. Neomycin selection.
    Depositor
    Addgene Research Program, Melina Fan
    Insert
    Green Fluorescent Protein
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 21, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234464-plasmid-map-sequence-id-464634

    Enlarge

  4. pCS2-zH2B-GFP

    Plasmid
    #226561
    Purpose
    Express H2B-GFP in zebrafish.
    Depositor
    Yong Zhang
    Insert
    h2b (h2bc1 Zebrafish)
    Use
    Synthetic Biology; Zebrafish expression
    Tags
    EGFP
    Expression
    Mutation
    Promoter
    Available since
    March 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    226561-plasmid-map-sequence-id-461800

    Enlarge

  5. pAIP1

    Plasmid
    #233685
    Purpose
    Engineered SUMO protease expression plasmid designed for efficient protease-mediated elution.
    Depositor
    Christopher Bahl
    Insert
    Ulp1-like SUMO protease
    Use
    Tags
    CAHS-107838 and HisTag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 4, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    233685-plasmid-map-sequence-id-463803

    Enlarge

  6. pZP05

    Plasmid
    #232319
    Purpose
    pZP05 was constructed by cloning the oriFn-repA fragment from pCWU6 into the smaller suicide plasmid pCM-galK, facilitating replication in Fusobacterium nucleatum
    Depositor
    Chenggang Wu
    Insert
    Ori-repA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232319-plasmid-map-sequence-id-462197

    Enlarge

  7. 3'dmDR-CMV-CasRx-bGHpolyA

    Plasmid
    #229556
    Purpose
    Expresses CasRx (RfxCas13d) with double processed direct repeats (15nt) and a non-target spacer in between at 3' end
    Depositor
    Omar Akbari
    Insert
    CasRx
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    229556-plasmid-map-sequence-id-455012

    Enlarge

  8. 1203U-U6-gRNA-pCMV-wtCas9-NG-P2A-mcherry-3'dmDR

    Plasmid
    #221448
    Purpose
    Expresses wtCas9-NG with double processed direct repeats (15nt) and a non-target spacer in between at 3' end, mCherry tag, and the gRNA targeting HEK293T-site3
    Depositor
    Omar Akbari
    Inserts
    wtCas9-NG
    mCherry
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221448-plasmid-map-sequence-id-437819

    Enlarge

  9. pLaG2-T7RNAPC

    Plasmid
    #216337
    Purpose
    Arabinose-inducible PBad promoter driving expression of the anti-eGFP nanobody LaG2 translationally fused to the C-terminal portion (residues 180-883) of T7RNAP
    Depositor
    Mark Styczynski
    Insert
    LaG2-T7RNAPC fusion
    Use
    Tags
    Expression
    Bacterial
    Mutation
    g2658a, g3915a
    Promoter
    Available since
    Jan. 17, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    216337-plasmid-map-sequence-id-456751

    Enlarge

  10. pBS-ACTB-C200-T7

    Plasmid
    #229850
    Purpose
    Donor vector for the ACTB site containing T7 sequence for detection of HR products.
    Depositor
    Natsuko Chiba
    Insert
    Partial sequence of ACTB gene
    Use
    Hr donor vector for human actb gene.
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    229850-plasmid-map-sequence-id-457516

    Enlarge

  11. pCG-scr

    Plasmid
    #229847
    Purpose
    Expresses wild-type Cas9 and scrambled gRNA.
    Depositor
    Natsuko Chiba
    Insert
    guide RNA with scrambled sequence
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    229847-plasmid-map-sequence-id-457515

    Enlarge

  12. pDual_dsCas9_Venus_hs_NC_chr1

    Plasmid
    #214682
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)
    Depositor
    Kevin Janes
    Insert
    dgRNA_Chr1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214682-plasmid-map-sequence-id-428816

    Enlarge

  13. pDual_dsCas9_Venus_hs_NC_chr2

    Plasmid
    #214683
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human non-coding DNA region of chromosome 2 (negative control)
    Depositor
    Kevin Janes
    Insert
    dgRNA_Chr2
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214683-plasmid-map-sequence-id-428804

    Enlarge

  14. pDual_dsCas9_Venus_hs_TP73

    Plasmid
    #214685
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human TP73
    Depositor
    Kevin Janes
    Insert
    dgRNA_TP73 (TP73 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214685-plasmid-map-sequence-id-428818

    Enlarge

  15. pDual_dsCas9_BFP_hs_NC_chr1

    Plasmid
    #214686
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-BFP with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)
    Depositor
    Kevin Janes
    Insert
    dgRNA_Chr1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214686-plasmid-map-sequence-id-428810

    Enlarge

  16. pDual_dsCas9_BFP_hs_TP73

    Plasmid
    #214687
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-BFP with two sgRNA sequences against human TP73
    Depositor
    Kevin Janes
    Insert
    dgRNA_TP73 (TP73 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214687-plasmid-map-sequence-id-428806

    Enlarge

  17. pOliSpec5'

    Plasmid
    #228551
    Purpose
    Template for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec3' and oligo-spacer amplifications, to clone gRNA plasmid
    Depositor
    Jeferson Gross
    Insert
    Truncated 5' region of aadA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228551-plasmid-map-sequence-id-454860

    Enlarge

  18. pOliSpec3'

    Plasmid
    #228550
    Purpose
    Template for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec5' and oligo-spacer amplifications, to clone gRNA plasmid.
    Depositor
    Jeferson Gross
    Insert
    Truncated 3' region of aadA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228550-plasmid-map-sequence-id-454859

    Enlarge

  19. pHR-TRE-miRFP703_PGK-Puro

    Plasmid
    #216667
    Purpose
    Expresses tTa-inducible miRFP703, with puromycin resistance
    Depositor
    Leonardo Morsut
    Inserts
    miRFP703
    PuroR
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    216667-plasmid-map-sequence-id-432197

    Enlarge

  21. pAAV-phSyn-flex-SypHaloTag

    Plasmid
    #172280
    Purpose
    Cre-dependent HaloTag labeling of neuronal presynaptic terminals from the synapsin promoter.
    Depositor
    Danny Winder
    Insert
    synaptophysin-HaloTag
    Use
    AAV and Cre/Lox
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    rat synapsin
    Available since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    172280-plasmid-map-sequence-id-337805

    Enlarge

  22. MDH2_EC

    Plasmid
    #224146
    Purpose
    IPTG induces expression of C. elegans protein MDH-2 with a N-terminal His tag.
    Depositor
    Katherine Walstrom
    Insert
    MDH-2 (mdh-2 Nematode)
    Use
    Tags
    His tag, Xpress tag, enterokinase cleaveage site
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    224146-plasmid-map-sequence-id-444697

    Enlarge

  23. pIND4-GFP-MmXYRS

    Plasmid
    #222745
    Purpose
    Expresses sfGFP and aaRS-tRNA pair for incorporating haloalkane crosslinker
    Depositor
    Steven Boxer
    Inserts
    sfGFP
    MmXYRS
    Mm Pyl tRNA
    Use
    Tags
    6xHis
    Expression
    Mutation
    Contains the following substitutions: V346A, W348…
    Promoter
    rrnB
    Available since
    Sept. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    222745-plasmid-map-sequence-id-442565

    Enlarge

  24. pGEX-6p1-GST-Ubiquitin

    Plasmid
    #223236
    Purpose
    Bacterial expression construct for GST-Tagged Ubiquitin production and purification
    Depositor
    Weixing Zhao
    Insert
    Ubiquitin
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    tac
    Available since
    Aug. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    223236-plasmid-map-sequence-id-443802

    Enlarge

  25. H2B-Electra2

    Plasmid
    #218929
    Purpose
    Electra2 fused to human H2B protein for nuclear localization
    Depositor
    Amro Hamdoun
    Insert
    H2B-Electra2 (H2B Synthetic, Human, Entacmaea quadricolor)
    Use
    Synthetic Biology
    Tags
    Electra2 and H2B
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    218929-plasmid-map-sequence-id-433882

    Enlarge

  26. LvPolyUb

    Plasmid
    #218982
    Purpose
    L.variegatus polyubiquitin-C promoter
    Depositor
    Amro Hamdoun
    Insert
    L.variegatus polyubiquitinC promoter
    Use
    Sea urchin
    Tags
    Expression
    Mutation
    Promoter
    L.variegatus polyubiquitinC promoter
    Available since
    Aug. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    218982-plasmid-map-sequence-id-433881

    Enlarge

  27. pet28a+_Yjjg

    Plasmid
    #220228
    Purpose
    Bacterial expression of Yjjgin E. coli BL21_DE3 (pLys) or similar
    Depositor
    Bernd Nidetzky
    Insert
    Pyrimidine 5'-nucleotidase YjjG (yjjG E.coli)
    Use
    Tags
    Histag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    220228-plasmid-map-sequence-id-439539

    Enlarge

  28. MUT_S1-spacer-H1

    Plasmid
    #218399
    Purpose
    3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron following a neutral spacer, and AG>GG mutation in final 3' splice site
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetracycline-responsive promoter
    Available since
    June 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    218399-plasmid-map-sequence-id-436404

    Enlarge

  29. MUT_S1-H1,NR

    Plasmid
    #218395
    Purpose
    3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, both randomly mutated to avoid sequence repeats, and AG>GG mutation in final 3' splice site
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetracycline-responsive promoter
    Available since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    218395-plasmid-map-sequence-id-433911

    Enlarge

  30. 1167A

    Plasmid
    #218233
    Purpose
    HDR plasmid for inserting Ceratitis capitata transformer female intron into the white pupae gene
    Depositor
    Omar Akbari
    Insert
    putative metabolite transport protein
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    May 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    218233-plasmid-map-sequence-id-433264

    Enlarge

  31. pET28-Flag-RBM24

    Plasmid
    #208621
    Purpose
    for E. coli expression of Flag tagged human RBM24 (ENST00000379052)
    Depositor
    Quanfu Ma
    Insert
    human RBM24 (ENST00000379052) coding region (RBM24 Human)
    Use
    Tags
    6xHis and Flag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    208621-plasmid-map-sequence-id-433637

    Enlarge

  32. pET-29b(+)_notufA-EmEndoF2-CBD-His

    Plasmid
    #218197
    Purpose
    Bacterial expression of a His-tagged Chitin Binding fusion of Elizabethkingia miricola EndoF2
    Depositor
    Florence Pojer
    Insert
    EndoF2
    Use
    Tags
    CBD-His
    Expression
    Bacterial
    Mutation
    codon optimized Ecoli, residues 45-335
    Promoter
    T7
    Available since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    218197-plasmid-map-sequence-id-431793

    Enlarge

  33. pAG426-GAL-Prepro-3x-Beta-Amyloid

    Plasmid
    #181730
    Purpose
    Galactose inducible expression of Prepro-3x-Beta-Amyloid
    Depositor
    Alejandro Chavez
    Insert
    Prepro-3x-Beta-Amyloid
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    181730-plasmid-map-sequence-id-431127

    Enlarge

  34. pAG426-GAL-Kar2-Beta-Amyloid

    Plasmid
    #181707
    Purpose
    Galactose inducible expression of Kar2-Beta-Amyloid
    Depositor
    Alejandro Chavez
    Insert
    Kar2-Beta-Amyloid
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    181707-plasmid-map-sequence-id-430950

    Enlarge

  35. E3_pENTR_L3-L2_NI_G1333-DddA11-N

    Plasmid
    #191609
    Purpose
    ptpTALECD_v2mod vector assembly, Entry clone 1 with attL2 and L3
    Depositor
    Shin-ichi Arimura
    Insert
    C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    191609-plasmid-map-sequence-id-379112

    Enlarge

  36. E3_pENTR_L3-L2_NN_G1333-DddA11-N

    Plasmid
    #191610
    Purpose
    ptpTALECD_v2mod vector assembly, Entry clone 1 with attL2 and L3
    Depositor
    Shin-ichi Arimura
    Insert
    C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    191610-plasmid-map-sequence-id-379111

    Enlarge

  37. E1_pENTR_L1-L4_HD_G1333-DddA11-C

    Plasmid
    #191599
    Purpose
    ptpTALECD_v2mod vector assembly, Entry clone 1 with attL1 and L4
    Depositor
    Shin-ichi Arimura
    Insert
    C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    191599-plasmid-map-sequence-id-379206

    Enlarge

  38. E1_pENTR_L1-L4_NG_G1333-DddA11-C

    Plasmid
    #191600
    Purpose
    ptpTALECD_v2mod vector assembly, Entry clone 1 with attL1 and L4
    Depositor
    Shin-ichi Arimura
    Insert
    C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    191600-plasmid-map-sequence-id-379205

    Enlarge

  39. E1_pENTR_L1-L4_NI_G1333-DddA11-C

    Plasmid
    #191601
    Purpose
    ptpTALECD_v2mod vector assembly, Entry clone 1 with attL1 and L4
    Depositor
    Shin-ichi Arimura
    Insert
    C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    191601-plasmid-map-sequence-id-379204

    Enlarge

  40. E1_pENTR_L1-L4_NN_G1333-DddA11-C

    Plasmid
    #191602
    Purpose
    ptpTALECD_v2mod vector assembly, Entry clone 1 with attL1 and L4
    Depositor
    Shin-ichi Arimura
    Insert
    C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    191602-plasmid-map-sequence-id-379199

    Enlarge

Showing: 5681 - 5720 of 16254 results