We narrowed to 9,586 results for: Coli
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Plasmid#197975PurposeE. coli expression of HtrA2(delta133)-6HisDepositorAvailable SinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only
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pnEA-NpM-HsNot4_400-424_AG
Plasmid#148790PurposeE. coli of HsNot4_400-424DepositorInsertHsNot4_400-424 (CNOT4 Human)
ExpressionBacterialAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
P2061
Plasmid#186299PurposeExpresses LexA-HBD-B112 under the control of pAct1 and Cas9 under the control of LexA-HBD-B112+Beta-estradiol inducible promoterDepositorInsertsLexA-HBD-B112
Cas9
UseCRISPR and Synthetic BiologyExpressionBacterial and YeastPromoter2xLexop-minimalCyc1 and PACT1Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD70Tet(DadRmt5)-N-3xFLAG-DadR(ΔDBD)
Plasmid#191641PurposeE. coli - Eggerthella lenta shuttle plasmid (TetR), N-3xFLAG-dadR(ΔDBD) complementationDepositorInsertDadR
Tags3xFLAGExpressionBacterialMutationΔDBDAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD70Tet(DadRmt6)-N-3xFLAG-DadR(ΔTM)
Plasmid#191642PurposeE. coli - Eggerthella lenta shuttle plasmid (TetR), N-3xFLAG-dadR(ΔTM) complementationDepositorInsertDadR
Tags3xFLAGExpressionBacterialMutationΔTMAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD70Tet(DadRmt7)-N-3xFLAG-DadR(linker)
Plasmid#191643PurposeE. coli - Eggerthella lenta shuttle plasmid (TetR), N-3xFLAG-dadR(linker region shuffled) complementationDepositorInsertDadR
Tags3xFLAGExpressionBacterialMutationLinker region sequence shuffledAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD70Tet(DadH)-Pdadh-DadhABC(A2)
Plasmid#191644PurposeE. coli - Eggerthella lenta shuttle plasmid (TetR), native promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strainDepositorInsertDopamine dehydroxylase
ExpressionBacterialAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET29a(+)::9xhis-Tse5
Plasmid#192962PurposeHeterologous expression of Tse5 in E. coli Lemo21 cells for purification of Tse5-CTDepositorInsertTse5
Tags9xhis + TEV tagExpressionBacterialAvailable SinceDec. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP7_rpoA-U
Plasmid#190974PurposeTest the U-to-C editing activity of KP7 on AtrpoA editing site, in E. coliDepositorInsertPLS-KP7
TagsHisExpressionBacterialPromoterT7Available SinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP2-E67A_rpoA-U
Plasmid#190959PurposeTest the U-to-C editing activity of KP2:E67A on AtrpoA editing site, in E. coliDepositorInsertPLS-KP2
TagsHisExpressionBacterialMutationDYW-KP:E67APromoterT7Available SinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP6-A66S_rpoA-C
Plasmid#190968PurposeTest the C-to-U editing activity of KP6:A66S on AtrpoA editing site, in E. coliDepositorInsertPLS-KP6
TagsHisExpressionBacterialMutationDYW-KP:A66SPromoterT7Available SinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP1_rpoA-U
Plasmid#190955PurposeTest the U-to-C editing activity of KP1 on AtrpoA editing site, in E. coliDepositorInsertPLS-KP1
TagsHisExpressionBacterialPromoterT7Available SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP2-A66S_rpoA-U
Plasmid#190957PurposeTest the U-to-C editing activity of KP2:A66S on AtrpoA editing site, in E. coliDepositorInsertPLS-KP2
TagsHisExpressionBacterialMutationDYW-KP:A66SPromoterT7Available SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP3-A66S_rpoA-U
Plasmid#190961PurposeTest the U-to-C editing activity of KP3:A66S on AtrpoA editing site, in E. coliDepositorInsertPLS-KP3
TagsHisExpressionBacterialMutationDYW-KP:A66SPromoterT7Available SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP3-E67A_rpoA-U
Plasmid#190963PurposeTest the U-to-C editing activity of KP3:E67A on AtrpoA editing site, in E. coliDepositorInsertPLS-KP3
TagsHisExpressionBacterialMutationDYW-KP:E67APromoterT7Available SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP4_rpoA-U
Plasmid#190964PurposeTest the U-to-C editing activity of KP4 on AtrpoA editing site, in E. coliDepositorInsertPLS-KP4
TagsHisExpressionBacterialPromoterT7Available SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP6-E67A_rpoA-C
Plasmid#190972PurposeTest the C-to-U editing activity of KP6:E67A on AtrpoA editing site, in E. coliDepositorInsertPLS-KP6
TagsHisExpressionBacterialMutationDYW-KP:E67APromoterT7Available SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP5_rpoA-U
Plasmid#190965PurposeTest the U-to-C editing activity of KP5 on AtrpoA editing site, in E. coliDepositorInsertPLS-KP5
TagsHisExpressionBacterialPromoterT7Available SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEUK035
Plasmid#180827PurposeT7-inducible expression construct to produce SwGdmB (HY78_09235) in E. coliDepositorArticleInsertSwGdmB
Tags6X HisExpressionBacterialPromoterT7Available SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEUK036
Plasmid#180828PurposeT7-inducible expression construct to produce CnGdmA (CNE_RS35785) in E. coliDepositorArticleInsertCnGdmA
Tags6X HisExpressionBacterialPromoterT7Available SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEUK066
Plasmid#180830PurposeT7-inducible expression construct to produce NaGdmA (SARO_RS07455) in E. coliDepositorArticleInsertNaGdmA
Tags6X HisExpressionBacterialPromoterT7Available SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSET-TorA-6xHis-M13-pHCountdown-Calmodulin
Plasmid#182305PurposeE. coli plasmid encoding a fusion between pH-Countdown and the calcium-binding domains of GECO 1.2 with a hexahistidine tag, targeted to the periplasm via the TorA presequence.DepositorInsertpH-Countdown insertion into GECO 1.2 calcium sensing domains
TagsA modified TorA periplasmic targeting sequence pl…ExpressionBacterialMutationSpontaneous mutation of calmodulin's penulti…Promotert7Available SinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
MBP_Lims (MBP_LIM1234)
Plasmid#182042PurposeFor expression of human paxillin LIM domains 1-4 with N-terminal GST fusion protein in E. coli.DepositorAvailable SinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
GST_LIM1224
Plasmid#182041PurposeFor expression of human paxillin LIM domains 1, 2, 2 and 4 with N-terminal GST fusion protein in E. coli.DepositorAvailable SinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
GST_LIM1334
Plasmid#182040PurposeFor expression of human paxillin LIM domains 1, 3, 3 and 4 with N-terminal GST fusion protein in E. coli.DepositorAvailable SinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
GST_LIM234
Plasmid#182039PurposeFor expression of human paxillin LIM domains 2-4 with N-terminal GST fusion protein in E. coli.DepositorAvailable SinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pThuAsol
Plasmid#171675PurposepThuAsol expression in E. coliDepositorInsertSUMO-TEV-ThuAsol
TagsHis-tag and SUMO-tagExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-4
Plasmid#163130PurposepLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PhoA signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterT7Available SinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-3
Plasmid#163129PurposepLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding OmpA signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterT7Available SinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-2
Plasmid#163128PurposepLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding MalE signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterT7Available SinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-5
Plasmid#163131PurposepLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding DsBA signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterT7Available SinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSF-ChPylTMSK
Plasmid#163915PurposetRNA synthetase/tRNA pair for the in vivo incorporation of N6-(((Trimethylsilyl)methoxy)carbonyl)-L-lysine (TMSK), into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
ExpressionBacterialPromoterT7Available SinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-optimizedNedd4.2F823D
Plasmid#165105Purposebacterial expression of a GST fusion of Human Nedd4.2d F823DDepositorInsertNedd4.2d F823D (NEDD4L Human)
TagsGST from plasmid.ExpressionBacterialMutationcodon optimized for E. coli expression; F823D; …Available SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbr-XMod-Doc (WT, S199AzF)-HIS
Plasmid#153443PurposeE. coli expression (amber suppression) of Rc. XDocB with serine at position 199 replaced with amber codon.DepositorInsertRc.XDocB
Tags6xHis and ybbr tagExpressionBacterialMutationSerine 199 was mutated to amber codon (TAG)PromoterT7 promoterAvailable SinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3_mTq-Tat (c-plasmid)
Plasmid#127635PurposeEncodes a fluorecent protein with an RNA binding peptide: mTurquoise2-tat.Expression with constitutive E. coli RNAP promoter (J23106), ribozyme PlmJ and RBS BBa_B0034.DepositorInsertmTurquoise2
UseSynthetic BiologyTagsRNA binding peptide: PCP and RNA binding peptide:…ExpressionBacterialAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCool-human TSC1 (939-992)
Plasmid#132618Purposeexpresses human TSC1 (939-992) in E. coliDepositorInsertTSC1 (939-992)
ExpressionBacterialAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBEAST_J23101-bphD
Plasmid#128138PurposeThe cell-free adapted backbone, pBEAST, expressing bphD (the enzyme converting 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate to benzoate) under control of the constitutive promoter J23101, and RBS B0032DepositorInsertbphD
UseSynthetic BiologyExpressionBacterialPromoterJ23101Available SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0.066
Plasmid#119666PurposeLevel 0 part. Terminator from pheA gene from E. coli.DepositorInsertTpheA-1
UseSynthetic BiologyAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0.074
Plasmid#119663PurposeLevel 0 part. Terminator from Cysteine synthase A gene (cysK) from E. coli.DepositorInsertECK120048902
UseSynthetic BiologyAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0.071
Plasmid#119662PurposeLevel 0 part. Terminator from tatABCD operon from E. coli.DepositorInsertECK120030798
UseSynthetic BiologyAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0.069
Plasmid#119661PurposeLevel 0 part. Terminator from arcA gene from E. coli.DepositorInsertECK120026481
UseSynthetic BiologyAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0.073
Plasmid#119660PurposeLevel 0 part. Terminator from rplJL-rpoBC,rplKAJL-rpoBC,rpoBC gene cluster from E. coli.DepositorInsertECK120010869
UseSynthetic BiologyAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0.065
Plasmid#119659PurposeLevel 0 part. Terminator araBAD operon from E. coli.DepositorInsertECK120010860
UseSynthetic BiologyAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0.068
Plasmid#119658PurposeLevel 0 part. Terminator for clpPX gene from E. coli.DepositorInsertECK120010850
UseSynthetic BiologyAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0.072
Plasmid#119657PurposeLevel 0 part. Terminator from hyaABCDEF operon from E. coli.DepositorInsertECK120010842
UseSynthetic BiologyAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0.077
Plasmid#119656PurposeLevel 0 part. Terminator from rpoH gene from E. coli.DepositorInsertECK120010841-R
UseSynthetic BiologyAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0.064
Plasmid#119655PurposeLevel 0 part. Terminator from hupB gene from E. coli.DepositorInsertECK120010820
UseSynthetic BiologyAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0.070
Plasmid#119652PurposeLevel 0 part. Terminator, Bi-directional terminator for yciA/tonA genes from E. coli.DepositorInsertBBa_B0061
UseSynthetic BiologyAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_CtCHAD-H253A-R256A-R260A
Plasmid#127788PurposeExpresses a CtCHAD mutant protein in E. coli cellsDepositorInsertCtCHAD (CT0884 Chlorobium tepidum)
Tags6xHis tag + TEV cleavage siteExpressionBacterialMutationResidues 208-522 (CHAD domain), mutations in His2…Available SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_CtCHAD-H253A-R256A-R260A-R296A
Plasmid#127789PurposeExpresses a CtCHAD mutant protein in E. coli cellsDepositorInsertCtCHAD (CT0884 Chlorobium tepidum)
Tags6xHis tag + TEV cleavage siteExpressionBacterialMutationResidues 208-522 (CHAD domain), mutations in His2…Available SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only